URM1 (NM_030914) Human Untagged Clone

SKU SC122994

URM1 (untagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: URM1
• Synonyms: C9orf74
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_030914 edited; GCGAGCTCGGCTTCCTCAACATGGCTGCGCCCTTGTCAGTGGAGGTGGAGTTCGGAGGTG; GTGCGGAGCTCCTGTTTGACGGTATTAAGAAACATCGAGTCACTTTGCCTGGACAGGAGG; AACCCTGGGACATCCGGAACCTGCTCATCTGGATCAAGAAGAATTTGCTAAAAGAGCGGC; CAGAGTTGTTCATCCAGGGAGACAGCGTGCGGCCAGGAATTCTGGTGCTGATTAACGATG; CCGACTGGGAGCTACTGGGTGAGCTGGACTACCAGCTTCAGGACCAGGACAGCGTCCTCT; TCATCTCCACTCTGCACGGCGGCTGAGGGCCCTTCTCTGGGCCTGGGCACCCTTAGAGGG; GAGAACGAAGCAATCAGACATCCCCTTGGGCCCTGCTTCCAGGTCTCCCTGTCCCCCTTG; CCTGCCTTCTTCCCTGCTCTGTCCCCTAAGCTCCCTCCAGGCAGGGAAAAGAGGCCAGGT; GCTAAAAATGAGCCTTTCTCAAGCACGTGAGCAGCGGAAGGCAGACAGGCGCCAGAGCCC; AGCACTCCCTTTTCCAGCAGCTGTGGTGGGGGAGGGTTCCCCTCCAGTTTGTCAAGAGTT; GAAGGAGGCTCTGTGGCCAGGTGACCTGGCTGCCTTCCACCCCTTGTACCTCAGTCTAAA; CATGGAGTGGCCGCTGACAAGGCGCTCCAGCCCCAGAGCCAGCGTCTTCATGGGGAAGAT; GAATGGACCTGAGTAGCTGAAGGAAGGCCCCTCCCTACCCAAAGACTGGAGGCTTCTCAG; CCTCAATTTCCCTGTCTGTACAGCTGAGGGCTCTGCCTGTCCCCCACTGCTATCAGTATG; GAACCCCAGCTGGGGTCCCCTATTGAGTGCCGACTCCCCCCACCGCCAGCAGCTGCTCCT; CCAGCCACACCCCTCCTGCTCCCCCCACCCCTAGCCCTTGACCCTGGCTGGCCTGCCCCG; CTCCACAGGCCACCAGATGGGCTCCTGAGACCCTCCCCAGGCTGCTTACAGCTCATTCTG; CTGGGGGTAGAGATGAGGGGAGGGAGTAAGTTAAACCTTGGACTAGCAAGTAGAAGCCTG; GGGGGATGCGTGTGCCTCAGTTTCCTCCTCCACAACTGAATATTTATAGTGGCTGAAAAC; TGGGGAGATACTTGATGGCGCGAATGTCCGTTTTCTCTCCCTTCCCACCTCCTGCAGGAA; GCAGGACGGGGCAGGCAGCACCTGGTAGGCACAGTGCTTTGCCCCTCCTCCCCTTCCCTT; CTGGAAGTCTTGGGGCCTCAGTGCTTGCAACAGCCGGCCTTGGGCAAATAAAAGACTAGG; TTGTTTACTAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030914 unedited; NNAAGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCGAG; CTCGGCTTCCTCAACATGGCTGCGCCCTTGTCAGTGGAGGTGGAGTTCGGAGGTGGTGCG; GAGCTCCTGTTTGACGGTATTAAGAAACATCGAGTCACTTTGCCTGGACAGGAGGAACCC; TGGGACATCCGGAACCTGCTCATCTGGATCAAGAAGAATTTGCTAAAAGAGCGGCCAGAG; TTGTTCATCCAGGGAGACAGCGTGCGGCCAGGAATTCTGGTGCTGATTAACGATGCCGAC; TGGGAGCTACTGGGTGAGCTGGACTACCAGCTTCAGGACCAGGACAGCGTCCTCTTCATC; TCCACTCTGCACGGCGGCTGAGGGCCCTTCTCTGGGCCTGGGCACCCTTAGAGGGGAGAA; CGAAGCAATCAGACATCCCCTTGGGCCCTGCTTCCAGGTCTCCCTGTCCCCCTTGCCTGC; CTTCTTCCCTGCTCTGTCCCCTAAGCTCCCTCCAGGCAGGGAAAAGAGGCCAGGTGCTAA; AAATGAGCCTTTCTCAAGCACGTGAGCAGCGGAAGGCAGACAGGCGCCAGAGCCCAGCAC; TCCCTTTTCCAGCAGCTGGGTGGGGGGANGGGGTTCCNCTCCAGTTTGTCAAGAGTTGAA; GGAGGCTCTGTGGCCAGGTGACCTGGCTGCCTTCCACCCCTTGTACCTCAGTCTAAACAT; GGAGTGGCCGCTGACAAGGCGCTCCAGCCCCAGAGCCAGCGTCTTCATGGGGAAGATGAA; TGGACCTGAGTAGCTGAAGGAAGGCCCCTCCCTACCCAAGACTGGAGGCTTCTCAGCCTC; AATTTCCCTGTCTGTACAGCTNGAGGCTCTGCCTGTCCCCCACTGCTATCAGTATGGA;
• Restriction Sites: Please inquire
• ACCN: NM_030914
• Insert Size: 1348 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030914.1, NP_112176.1
• RefSeq Size: 1348 bp
• RefSeq ORF: 306 bp
• Locus ID: 81605
• UniProt ID: Q9BTM9
• Cytogenetics: 9q34.11
• Summary: Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) lacks a segment in the 3' coding region, compared to variant 2, which results in a protein (isoform 1) with a distinct C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.