TUG (ASPSCR1) (NM_024083) Human Untagged Clone

SKU SC122950

ASPSCR1 (untagged)-Human alveolar soft part sarcoma chromosome region, candidate 1 (ASPSCR1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TUG
• Synonyms: ASPCR1; ASPL; ASPS; RCC17; TUG; UBXD9; UBXN9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024083 edited; AAATGGCGGCCCCGGCAGGCGGCGGAGGCTCCGCGGTGTCGGTGCTGGCCCCGAACGGCC; GGCGCCACACGGTGAAGGTGACGCCGAGCACCGTGCTGCTTCAGGTTCTGGAGGACACGT; GCCGGCGGCAGGACTTCAACCCCTGTGAATATGATCTGAAGTTTCAGAGGAGCGTGCTCG; ACCTTTCTCTCCAGTGGAGATTTGCCAACCTGCCCAACAATGCCAAGCTGGAGATGGTGC; CCGCTTCCCGGAGCCGTGAGGGGCCTGAGAACATGGTTCGCATCGCTTTGCAGCTGGACG; ATGGCTCGAGGTTGCAGGACTCTTTCTGTTCAGGCCAGACCCTCTGGGAGCTTCTCAGCC; ATTTTCCACAGATCAGGGAGTGCCTGCAGCACCCCGGCGGGGCCACCCCAGTCTGCGTGT; ACACGAGGGATGAGGTGACGGGTGAAGCTGCCCTGCGGGGCACGACGCTGCAGTCGCTGG; GCCTGACCGGGGGCAGCGCCACCATCAGGTTTGTCATGAAGTGCTACGACCCCGTGGGCA; AGACCCCAGGAAGCCTGGGCTCGTCAGCGTCGGCTGGCCAGGCAGCCGCCAGCGCTCCAC; TTCCCTTGGAATCTGGGGAGCTCAGCCGCGGCGACTTGAGCCGTCCGGAGGACGCGGACA; CCTCAGGGCCCTGCTGCGAGCACACTCAGGAGAAGCAGAGCACAAGGGCACCCGCAGCTG; CCCCCTTTGTTCCTTTCTCGGGTGGGGGACAGAGACAGGGGGGCCCTCCTGGGCCCACGA; GGCCTCTGACATCATCTTCAGCTAAGTTGCCGAAGTCCCTCTCCAGCCCTGGAGGCCCCT; CCAAGCCAAAGAAGTCCAAGTCGGGCCAGGATCCCCAGCAGGAGCAGGAGCAGGAGCGGG; AGCGGGATCCCCAGCAGGAGCAGGAGCGGGAGCGGCCCGTGGACCGGGAGCCCGTGGACC; GGGAGCCGGTGGTGTGCCACCCCGACCTGGAGGAGCGGCTGCAGGCCTGGCCAGCGGAGC; TGCCTGATGAGTTCTTTGAGCTGACGGTGGACGACGTGAGAAGACGCTTGGCCCAGCTCA; AGAGTGAGCGGAAGCGCCTGGAAGAAGCCCCCTTGGTGACCAAGGCCTTCAGGGAGGCGC; AGATAAAGGAGAAGCTGGAGCGCTACCCAAAGGTGGCTCTGAGGGTCCTGTTCCCCGACC; GCTACGTCCTACAGGGCTTCTTCCGCCCCAGCGAGACAGTGGGGGACTTGCGAGACTTCG; TGAGGAGCCACCTGGGGAACCCCGAGCTGTCATTTTACCTGTTCATCACCCCTCCAAAAA; CAGTCCTGGACGACCACACGCAGACCCTCTTTCAGGCGAACCTCTTCCCGGCCGCTCTGG; TGCACTTGGGAGCCGAGGAGCCGGCAGGTGTCTACCTGGAGCCTGGCCTGCTGGAGCATG; CCATCTCCCCATCTGCGGCCGACGTGCTGGTGGCCAGGTACATGTCCAGGGCCGCCGGGT; CCCCTTCCCCATTGCCAGCCCCTGACCCTGCACCTAAGTCTGAGCCAGCTGCTGAGGAGG; GGGCGCTGGTCCCCCCTGAGCCCATCCCAGGGACGGCCCAGCCCGTGAAGAGGAGCCTGG; GCAAGGTGCCCAAGTGGCTGAAGCTGCCGGCCAGCAAGAGGTGAGAGCTGCCAGCCTGAG; GTGCCCACTCCGCCAGCCACAGGACCACCTCCTCTGCCAGCAGGAATAAAGACTTGTGCA; TCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024083 unedited; GGGTTCACATTTTGTGATACGACTCACTATAGGGCGGGGCATAAATTCCTAACGAGGAAA; TGGCGGCCCCGGCAGGCGGCGGAGGCTCCGCGGTGTCGGTGCTGGCCCCGAACGGCCGGC; GCCACACGGTGAAGGTGACGCCGAGCACCGTGCTGCTTCAGGTTCTGGAGGACACGTGCC; GGCGGCAGGACTTCAACCCCTGTGAATATGATCTGAAGTTTCAGAGGAGCGTGCTCGACC; TTTCTCTCCAGTGGAGATTTGCCAACCTGCCCAACAATGCCAAGCTGGAGATGGTGCCCG; CTTCCCGGAGCCGTGAGGGGCCTGAGAACATGGTTCGCATCGCTTTGCAGCTGGACGATG; GCTCGAGGTTGCAGGACTCTTTCTGTTCAGGCCAGACCCTCTGGGAGCTTCTCAGCCATT; TTCCACAGATCAGGGAGTGCCTGCAGCACCCCGGCGGGGCCACCCCAGTCTGCGTGTACA; CGAGGGATGAGGTGACGGGTGAAGCTGCCCTGCGGGGCACGACGCTGCAGTCGCTGGGCC; TGACCGGGGGCAGCGCCACCATCAGGTTTGTCATGAAGTGCTACGACCCCGTGGGCAAGA; CCCCAGGAAGCCTGGGCTCGTCAGCGTCGGCTGGCCAGGCAGCCGCCAGCGCTCCACTTC; CCTTGGAATCTGGGGAGCTCAGCCGCGGCGACTTGAGCCGTCCGGAGGACGCGGACACCT; CAGGGCCCCTGCTGCGAGCACACTCAGGAGAAGCAGAGCACAAGGGCACCCGCAGCTGCC; CCCTTTGTCCTTTCTCGGGTGGGGACAGAGACAGGGGGGCCCTCCTGGGCCAACGAGGCC; TCTGACATCATCTTCAGCTAAGTTGCCGAATTCCCTCTCCAGCCCTGAAGGCCCCTTCAA; GCCCAAGAAGTCCAAGTA;
• Restriction Sites: Please inquire
• ACCN: NM_024083
• Insert Size: 1796 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024083.2, NP_076988.1
• RefSeq Size: 1858 bp
• RefSeq ORF: 1662 bp
• Locus ID: 79058
• UniProt ID: Q9BZE9
• Cytogenetics: 17q25.3
• Summary: The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (1) encodes the predominant protein (isoform 1).