ALG1 (NM_019109) Human Untagged Clone

CAT#: SC122883

ALG1 (untagged)-Human asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALG1
• Synonyms: CDG1K; hMat-1; HMAT1; HMT-1; HMT1; Mat-1; MT-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_019109 edited
  ACTGCTGCGGGCCAGCCAAGATGGCGGCCTCATGCTTGGTCCTGCTGGCGCTGTGTCTGC
  TGCTGCCGCTGCTGCTGCTGGGAGGATGGAAGCGCTGGCGCCGGGGGCGGGCGGCCCGGC
  ATGTAGTAGCGGTGGTGCTGGGCGACGTGGGCCGCAGCCCCCGTATGCAGTACCACGCGC
  TGTCGTTGGCCATGCACGGCTTCTCGGTGACCCTCCTGGGGTTCTGCAACTCCAAACCCC
  ATGATGAGCTCTTGCAGAACAACAGAATTCAGATTGTGGGGTTGACAGAACTTCAGAGTC
  TTGCAGTTGGGCCCCGAGTTTTCCAGTACGGAGTCAAAGTTGTACTTCAGGCTATGTACT
  TGCTGTGGAAGTTGATGTGGAGGGAGCCAGGTGCCTATATCTTTCTCCAGAACCCCCCAG
  GTCTGCCTAGCATTGCTGTCTGCTGGTTCGTGGGCTGCCTTTGTGGAAGCAAGCTCGTCA
  TTGACTGGCACAACTATGGCTACTCCATCATGGGTCTGGTGCATGGCCCCAACCATCCCC
  TCGTTCTGCTGGCCAAGTGGTACGAGAAGTTCTTTGGGCGCCTGTCCCACCTGAACCTGT
  GTGTTACCAATGCTATGCGAGAAGACCTGGCGGATAACTGGCACATCAGGGCTGTGACCG
  TCTACGACAAGCCCGCATCTTTCTTTAAAGAGACACCTCTGGACCTGCAGCACCGGCTCT
  TCATGAAGCTGGGCAGCATGCACTCTCCGTTCAGGGCCCGCTCAGAACCTGAGGACCCAG
  TCACGGAGCGGTCGGCCTTCACGGAGCGGGATGCTGGGAGCGGGCTGGTGACGCGTCTCC
  GTGAGCGGCCAGCCCTGCTGGTCAGCAGCACGAGCTGGACAGAGGACGAAGACTTCTCCA
  TCCTGCTGGCAGCTTTAGAAAAGTTTGAACAACTGACTCTTGATGGACACAACCTTCCTT
  CTCTCGTCTGTGTGATAACAGGCAAAGGGCCTATGAGGGAGTATTATAGCCGCCTCATCC
  ACCAGAAGCACTTCCAGCACATCCAGGTCTGCACCCCCTGGCTGGAGGCCGAGGACTACC
  CCCTGCTTCTAGGGTCGGCGGACCTGGGTGTCTGTCTGCACACGTCCTCCAGTGGCCTGG
  ACCTGCCCATGAAGGTGGTGGACATGTTCGGGTGCTGTTTGCCTGTGTGTGCTGTGAACT
  TCAAGTGTTTACATGAGCTGGTGAAACATGAAGAAAATGGCCTGGTCTTTGAGGACTCAG
  AGGAACTGGCAGCTCAGCTGCAGATGCTTTTCTCAAACTTTCCTGATCCTGCGGGCAAGC
  TAAACCAGTTCCGGAAGAACCTGCGGGAGTCGCAGCAGCTCCGATGGGATGAGAGCTGGG
  TGCGGACTGTGCTCCCTTTGGTTATGGACACATAACTCCTGGGCCAGAGGCTATAAAACC
  CCAGGACCCCTGCTGTCCTTCCCGCAGCTTCTTCTTGGAGTCTCAGGGCAAACCCTTTCG
  AGCAGCACCTCCCAGTGGCCAGAAGCTGAAATGACAGCAGTGGTACTACCTGGTAAAAGA
  ATTGGTTCTGTGACCCGGGAAGCTTTGGTTGGCCTTGATTTCTTCTCTGGAGGCTTGGAA
  ACGCTTCCTCTCTTCTTCTGTTCTTCACGCCCCATGCCCCTGCTAGCGTATTACTGTTCT
  GTGACTTCCCTGTGACCTCTGCAGAACTCCTCATCCTGCGTTTGGTCTCCAGGTGTCCCC
  TTTCTGCCGTGTTCCTAACATTTTGATTCCTGTCTTGAAAAAAGCACCTGCTGCACCGTA
  AGCCCAGGGATGTGGCAGCTGCAGTGGGCTTGGCTTTGTGAGGAACTGAGTGTGTCCACG
  TTGGGGGAACATCATACTTGATACACACGTTTTTATTTGCACAAAGAAAATGCTATTTTT
  GGAGCCAGAATTTTCATGTCTGATTTATGGTGATTTTCTTAAGAACCAGAACTGCTGGCA
  GAAAGGGGGCACCCACACGCTTAGATAGCCGATGTCTTATTAGAGGGCAGTTTGTGGTTC
  CTGATTTGGAAATTAACATTCTCCAAACATTCCAGTCCAATGAAAGTTTTATCCGCTTTC
  CCATATAAAAATTCTTCCCACGAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019109 unedited
  NNGGAGTTCACATTTGTATACGACTCATATAGGCGGCCGCATAACTTCGTATAGCATACA
  TTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGACTG
  CTGCGGGCCAGCCAAGATGGCGGCCTCATGCTTGGTCCTGCTGGCGCTGTGTCTGCTGCT
  GCCGCTGCTGCTGCTGGGAGGATGGAAGCGCTGGCGCCGGGGGCGGGCGGCCCGGCATGT
  AGTAGCGGTGGTGCTGGGCGACGTGGGCCGCAGCCCCCGTATGCAGTACCACGCGCTGTC
  GTTGGCCATGCACGGCTTCTCGGTGACCCTCCTGGGGTTCTGCAACTCCAAACCCCATGA
  TGAGCTCTTGCAGAACAACAGAATTCAGATTGTGGGGTTGACAGAACTTCAGAGTCTTGC
  AGTTGGGCCCCGAGTTTTCCAGTACGGAGTCAAAGTTGTACTTCAGGCTATGTACTTGCT
  GTGGAAGTTGATGTGGAGGGAGCCAGGTGCCTATATCTTTCTCCAGAACCCCCCAGGTCT
  GCCTAGCATTGCTGTCTGCTGGTTCGTGGGCTGCCTTTGTGGAAGCAAGCTCGTCATTGA
  CTGGCACAACTATGGCTACTCCATCATGGGTCTGGTGCATGGCCCCAACCATCCCCTCGT
  TCTGCTGGCCAAGTGGTACGAGAAGTTCTTTGGGCGCCTGTCCCACCTGAACCTGTGTGT
  TACCAATGCTATGCGAGAAGACCTGGCGGATAACTGGCACATCAAGGCTGTGACCGTCTA
  CGACAAGCCGCATCTTTCTTTAAGAGACACCTCTGGACTGCAGCACCGGCTCTTCATGAG
  CTGGGCANCATGCACTCTCCGTTCAGGGCCCGCTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_019109
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019109.3, NP_061982.3
• RefSeq Size: 2149 bp
• RefSeq ORF: 1395 bp
• Locus ID: 56052
• UniProt ID: Q9BT22
• Cytogenetics: 16p13.3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).