HAND1 (NM_004821) Human Untagged Clone

SKU SC122690

HAND1 (untagged)-Human heart and neural crest derivatives expressed 1 (HAND1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HAND1
• Synonyms: bHLHa27; eHand; Hxt; Thing1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004821 edited; GTCCGCAGAAGGGTTAAACAGGTCTTTGGGCTTCGGCGACCTCGCCCGCGGCAGAAACCG; GTAAGAAGACAGTGGGCTGCGCGTCTCATTTTCAGCCTTGCCCGGACTCTCCCAAAGCCG; GCGCCCAGTAGTGGCTCCAGAGCCCACAGGTGGCCCCCGGCAGTCTCTGGGGCGCATGGA; GCGGCGTTAATAGGGCTGGCGGCGCAGGCCAGTAGCCGCTCCAACATGAACCTCGTGGGC; AGCTACGCACACCATCACCACCATCACCACCCGCACCCTGCGCACCCCATGCTCCACGAA; CCCTTCCTCTTCGGTCCGGCCTCGCGCTGTCATCAGGAAAGGCCCTACTTCCAGAGCTGG; CTGCTGAGCCCGGCTGACGCTGCCCCGGACTTCCCTGCGGGCGGGCCGCCGCCCGCGGCC; GCTGCAGCCGCCACCGCCTATGGTCCTGACGCCAGGCCTGGGCAGAGCCCCGGGCGGCTG; GAGGCGCTTGGCGGCCGTCTTGGCCGGCGGAAAGGCTCAGGACCCAAGAAGGAGCGGAGA; CGCACTGAGAGCATTAACAGCGCATTCGCGGAGTTGCGCGAGTGCATCCCCAACGTGCCG; GCCGACACCAAGCTCTCCAAGATCAAGACTCTGCGCCTAGCCACCAGCTACATCGCCTAC; CTGATGGACGTGCTGGCCAAGGATGCACAGTCTGGCGATCCCGAGGCCTTCAAGGCTGAA; CTCAAGAAGGCGGATGGCGGCCGTGAGAGCAAGCGGAAAAGGGAGCTGCAGCAGCACGAA; GGTTTTCCTCCTGCCCTGGGCCCAGTCGAGAAGAGGATTAAAGGACGCACCGGCTGGCCG; CAGCAAGTCTGGGCGCTGGAGTTAAACCAGTGAGCCGAGGCCCGCGCCGAGGACCTGGCC; AGGCCAGCCACTCCTGAAGCCCCGGGAGGAGAGGAAGGCAGCGGCGAACGCCAGGCTCTG; GGCTCCGGCGACTGGTGCTACGCATCCCGCGGAGCTTCTGCTGAGCGCCGGCAGGTCGTC; GGCTGCAACCACACACTTGGATCGCACGTGCAATGTCCTTTGATTTTTTTTAATACATTA; AGAGAAAGAGAAATATATATATATCCACCCCCAGCCCAACCGAGGGCGGCCCTTGGCGGC; AACATGCAAGAAGGAGGGACTGTCGAACCCAAGGGCTCAAAGACGCACTCTTCCACCCTT; TTGGAGCGAATTTAGAACCTCAGCCCTATCTCCATTTCCCTATCTGGCTCTTTCTCTCTT; GTCCCTCCATATGATCCGCCCCGACGCCGTCTTCTCTAATTAAAATGCAATAAGGAATCA; ATTCTTTTCTGCCTGAGAAAGAGAACCAGACGCAGGAAGATGAAAGGCTGCCCTTTGTTC; TTCGAATCGTGGTGGTTTTATTTTATTTTTCTTTTTGTCGCTGCACTTCCTGTTTAGTTC; CAAGGGAAACACTTTCTCTCTTTCTCTGTCTCTCTCTTTTCTTCCTTCTTTTCCTTCCTT; TTTGTTTCTATCTAAATAAAAGCTTTCCCTGTGTTGGAAAGTTTTTATGTATTTAAACTA; CCTACCATGCCTGTTGTGCTCAGGTGTTTGTTCATCCTGCCATCCCCAACCCTTTTCTAC; CTCAAGTCTGTGTGACCACTCACAGCCCCCCTCCCTTCGCCAAAGCAGTGTCTATGCTCT; TGATTAATAAAACATTTTCTGAAATCAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004821 unedited; NNAAGTTGAATTTGTATACGACTCATATAGGCGGGCCGCGAATTCGCACGAGGGGTCCGC; AGAAGGGTTAAACAGGTCTTTGGGCTTCGGCGACCTCGCCCGCGGCAGAAACCGGTAAGA; AGACAGTGGGCTGCGCGTCTCATTTTCAGCCTTGCCCGGACTCTCCCAAAGCCGGCGCCC; AGTAGTGGCTCCAGAGCCCACAGGTGGCCCCCGGCAGTCTCTGGGGCGCATGGAGCGGCG; TTAATAGGGCTGGCGGCGCAGGCCAGTAGCCGCTCCAACATGAACCTCGTGGGCAGCTAC; GCACACCATCACCACCATCACCACCCGCACCCTGCGCACCCCATGCTCCACGAACCCTTC; CTCTTCGGTCCGGCCTCGCGCTGTCATCAGGAAAGGCCCTACTTCCAGAGCTGGCTGCTG; AGCCCGGCTGACGCTGCCCCGGACTTCCCTGCGGGCGGGCCGCCGCCCGCGGCCGCTGCA; GCCGCCACCGCCTATGGTCCTGACGCCAGGCCTGGGCAGAGCCCCGGGCGGCTGGAGGCG; CTTGGCGGCCGTCTTGGCCGGCGGAAAGGCTCAGGACCCAAGAAGGAGCGGAGACGCACT; GAGAGCATTAACAGCGCATTCGCGGAGTTGCGCGAGTGCATCCCCAACGTGCCGGCCGAC; ACCAAGCTCTCCAAGATCAAGACTCTGCGCCTAGCCACCAGCTACATCGCCTACCTGATG; GACGTGCTGGCCAAGGATGCACAGTCTGGCGATCCCNGAGCCTTCAAGGCTGAACTCAAN; AAAGCNGATGGCGGCCGTGAGAGCAGCCGGAAAAGGAGCTGCAGCAGCACGAAGGGTTTC; CTCCTGCCCTGNGGCCCCAGTCGAGAAGAGGATTAAAGGACCCACCGGCTGGCCGCA;
• Restriction Sites: Please inquire
• ACCN: NM_004821
• Insert Size: 1733 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004821.1, NP_004812.1
• RefSeq Size: 1750 bp
• RefSeq ORF: 648 bp
• Locus ID: 9421
• UniProt ID: O96004
• Cytogenetics: 5q33.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Summary: The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]