HLCS (NM_000411) Human Untagged Clone

SKU SC119915

HLCS (untagged)-Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HLCS
• Synonyms: HCS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119915 sequence for NM_000411 edited (data generated by NextGen Sequencing); ATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCAC; TTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATC; CTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCTGAGCAGGACGGTATGGAGCAT; GTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCC; TCTGGGAGTGAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCAC; CTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAGAACAGCACCATTGAGTCAGTC; AAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAG; AGTGTTGCTGATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAG; GCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAGGAAGCCCTCGGCCGGTTCCAC; GAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTG; CTGGAGGACAGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCT; ACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTCATGGCCTATCTTTCTCAGGGA; GGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAG; GGTGCACTGCACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGYGAGGTG; AAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAAGGCCCCGTCCGGCTCAGCCCC; GGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCT; TTTGGAACTCGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGC; TCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTCAAGTCAAGCAATTTTAGAAGA; TACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTT; CCTGCCTTAACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATG; CAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATAAAATCCGGCCAGCTCTCTCTT; AGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTG; ACCAACATGGAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTG; CAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAAGTGACCCCCACAACGATGCGT; CTCCTGGATGGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCC; CGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTGGCTGAGCCCTGTGGGATGTGCT; CTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTT; GTCCAGCATCTGATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAG; GATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTACAGTGACCTCATGAAGATC; GGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGT; GGATTTAATGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATAC; AATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGATTATCTCATCGCCAGAGTC; GTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTT; CCCCTTTATTACCGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAG; GGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGGCTTCCTCCAGGTTCACCAGGAG; GGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTC; ATCCTCCCCAAACGGCGGTAA; ; Clone variation with respect to NM_000411.6; 834 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000411 unedited; GTTCACATTCGGCACCAGGACGGGGCTGGGGCGCGGGCAGGATTTGGGGCTGCGCCGAGG; GCGTCCCGACCTGGCCCTTTGCCACGGTAGGTTCCGGCTCCCATGCCTCCGCTGCCATCT; GCGCTGTCCTTGGCGACTACGCCTTCACCCCCCTGGGTGCGTTTCAGCGGTCCCGGAGCC; TGGCGGGAGCGGGGCCAGGAGCGCTCCGCGGCCGGTATCAGGCCTGCAGTCCATTGAAGA; CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGC; ATTTGTGACGGAGAGCATTTGGGTACAAAGTGAGAATTTACAGAGATCATCCTCTTCAGA; AACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA; AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGAT; CCCTTTTAGTGCTCCCAGTAACCTTCCAAGATTGTCAAGTGGTCAGACTGTTGTTTGCCA; TTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC; AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCC; CAAAAGATTGTGTCGGTGCACTTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTC; TCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT; GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGACCCC; AAACCAAGGAGAGCAGTGCCTCTGGGAGTGACCTGCTGGGACAGTGACAGGGAAGGGGGC; CCCGTTGAGCATATCACCCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000411 unedited; GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACAAATGAATTGGAG; GAAAAGAAAATTCACCTACAACTCTAAATTAGATTTCCAGATGCATGGGCACGGACAGGC; AGCCGCGTCTCGGGGACGCCCGGCATTACCGCCGTTTGGGGAGGATGAGGTTTCTCAGCA; TGTCGAAGGAGTTGCCGTCCGGGTGCACAGTCACAACCTCGCCGCCCTCCTGGTGAACCT; GGAGGAAGCCAGAATCGTCCAGGCCAACGATGGACACCTTTGGTCCCTCTGCGCTGCCCA; GATGGACTTGCTGACCACTGTGGACCCAGTATCGGTAATAAAGGGGAAGGACGCTGTTGG; GCCCTTTGTCCTGAAACTCTTTGATCAGTTTCTCCAGCACAGTCACGACTCTGGCGATGA; GATAATCGGCTCTTAAGGGCTTCAGTTCTGCCTTGTGTTGTTTATTGTATTCTGTGATGA; GGTCGTTGATGCAAATGGTAGGGTTACTGTTAGTCACATTAAATCCACAGCCAATAAGTA; TATAAAATGTTTCTCCCATGAGTGTTGAGTTAACCAGAACTCCGCCGATCTTCATGAGGT; CACTGGTATAAATATCGTTGGGCCACTTCACTCGTAAGTTGATATCCTGATACTNCGGAA; TGGACCTCACTGCTTCCACGACAGCCACGGGACATCAGATGCTGGACAAACGGGATCCTC; TGTCCCAGCTGGGATCTCATTGGGATGGAGATGAGCANAGTNAAAAGAGCCATTCCACAG; GGCTTAACCACCATTCCCTCCCCGTCCTTTTGCCTCGGTCTGCCGGGCCGGGATCCTTAT; TAAACCATTTCTGCGGGGTTTGAAACATCACCCATCCCAGAACCCTTCTTGGGGGGGTAC; TTTGGAAAAAATATACTTTCCCACTGCTGGGCTGCATATTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000411
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000411.4, NP_000402.3
• RefSeq Size: 5991 bp
• RefSeq ORF: 2181 bp
• Locus ID: 3141
• UniProt ID: P50747
• Cytogenetics: 21q22.13
• Domains: BPL_C, BPL_LipA_LipB
• Protein Pathways: Biotin metabolism, Metabolic pathways
• Summary: This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011]; Transcript Variant: This variant (1) is the shortest transcript. Variants 1, 2 and 3 encode the same protein.