Myosin Light Chain 2 (MYL2) (NM_000432) Human Untagged Clone

SKU SC119884

MYL2 (untagged)-Human myosin, light chain 2, regulatory, cardiac, slow (MYL2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Myosin Light Chain 2
• Synonyms: CMH10; MLC-2s/v; MLC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000432 edited; ATGGCACCTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAACTCCAACGTGTTCTCCATG; TTCGAACAGACCCAAATCCAGGAATTTAAGGAGGCCTTCACTATCATGGACCAGAACAGG; GATGGCTTCATTGACAAGAACGATCTGAGAGACACCTTTGCTGCCCTTGGGCGAGTGAAC; GTGAAAAATGAAGAAATTGATGAAATGATCAAGGAGGCTCCGGGTCCAATTAACTTTACT; GTGTTCCTCACAATGTTTGGGGAGAAACTTAAGGGAGCGGACCCTGAGGAAACCATTCTC; AACGCATTCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCTGAAGGCTGATTACGTTCGG; GAAATGCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGGTTGACCAGATGTTCGCC; GCCTTCCCCCCTGACGTGACTGGCAACTTGGACTACAAGAACCTGGTGCACATCATCACC; CACGGAGAAGAGAAGGACTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000432 unedited; TTCAGGATTTGAATACNATTATATAGGGCGGCCGCGATTCGGCACGAGGCTCGGGAGGCA; GTGCTGGGTCCTTTCCACCATGGCACCTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAA; CTCCAACGTGTTCTCCATGTTCGAACAGACCCAAATCCAGGAATTTAAGGAGGCCTTCAC; TATCATGGACCAGAACAGGGATGGCTTCATTGACAAGAACGATCTGAGAGACACCTTTGC; TGCCCTTGGGCGAGTGAACGTGAAAAATGAAGAAATTGATGAAATGATCAAGGAGGCTCC; GGGTCCAATTAACTTTACTGTGTTCCTCACAATGTTTGGGGAGAAACTTAAGGGAGCGGA; CCCTGAGGAAACCATTCTCAACGCATTCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCT; GAAGGCTGATTACGTTCGGGAAATGCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGA; GGTTGACCAGATGTTCGCCGCCTTCCCCCCTGACGTGACTGGCAACTTGGACTACAAGAA; CCTGGTGCACATCATCACCCACGGAGAAGAGAAGGACTAGGAGGGGGCTCGCTGCTGCGC; CCTGNGCTCGTCTTTGCAGAGTGGTCCCTGCCCTCATCTCTCTCCCCCGAGTACCGCCTC; TGTCCCCTACCTTGTCTGTTAGCCATGTGGCTGCCCCATTTATCCACCTCCATCTTCTTT; GCAGCCTGNGTGGCTATGGGTACTTCGTGGGCGCACATCCTACAGTTGGAAAACCATCCC; GAAGCCATGTTCCATTAAACCAGAGGTCGTGTATTTGGTCAAAAAAAAAAAAAAAACTCG; ACTTTAATTGCGCCCGCGGCATTGCTGTTTCCTGAACAAACCCGGGTGGCATCCTTGGGA; CCCTCCCCATGGCTTTTCTGGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000432 unedited; CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGACCAAATACACGACCTCCTG; TTTATTGGAACATGGCCTCTGTGAGGATTTCCAACTGTAGGATGTGCGGCCACGAAGTAC; CCATAGCCACCCAGGCTGCAAAGAAGATGGAGGTGGATAAATGGGGCAGCCACATGGCTA; ACAGACAAGGTAGGGACAGAGGCGGTACTCGGGGGAGAGAGATGAGGGCAGGGACCACTC; TGCAAAGACGAGCCCAGGGCGCAGCAGCGAGCCCCCTCCTAGTCCTTCTCTTCTCCGTGG; GTGATGATGTGCACCAGGTTCTTGTAGTCCAAGTTGCCAGTCACGTCAGGGGGGAAGGCG; GCGAACATCTGGTCAACCTCCTCCTTGGAAAACCTCTCCGCCTGCGTGGTCAGCATTTCC; CGAACGTAATCAGCCTTCAGCACCCCTTTGCCTTCAGGGTCAAACACTTTGAATGCGTTG; AGAATGGTTTCCTCAGGGTCCGCTCCCTTAAGTTTCTCCCCAAACATTGTGAGGAACACA; GTAAAGTTAATTGGACCCGGAGCCTTCTTGATCATTTCATCAATTTCTTCATTTTTCACG; TTCACTCGCCCAAGGCCAGCAAAGGTGTCTCTCAGATCGTTCTTGCCAATGAAGCCATCC; CCTGTCTCGTCCATGATAGTGAAGGCCTCCTTAAATTTCTGGATTTGGGTCCTGTTCGAC; CATGGAGGACACGTTGGAGTTGGCGCCCCCCGTCTCTCTTCTTTGCTTTNNTTAGCNGCC; ATGCTGGAAAGGACCAATACTGCCTCCCGATCCTGGGCCCGATTTTGGCCCGCCTTTGTG; AGTTGTATTACCAATTTTGACGGTCACCTAACAACTCTGTTTAATAAACCTCCACCGTAC; ACCCTACCGCCCATTTGGGCAAGCGGGCGGGTTATTACAAACTTTGGAAGCCCGTGAATT; GGGGCCAACACTCCCTTGGACCCAGGGGTGGGAACTGTAACCCGGGGGCAAACGTTTTCC; GCCCTTGGGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000432
• Insert Size: 720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000432.1, NP_000423.1
• RefSeq Size: 813 bp
• RefSeq ORF: 501 bp
• Locus ID: 4633
• UniProt ID: P10916
• Cytogenetics: 12q24.11
• Domains: EFh
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
• Summary: Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]