p53 (TP53) (NM_000546) Human Untagged Clone
TP53 (untagged)-Human tumor protein p53 (TP53), transcript variant 1
|Product Name||p53 (TP53) (NM_000546) Human Untagged Clone|
|Type||Human Untagged Clone|
|Synonyms||BCC7; BMFS5; LFS1; P53; TRP53|
|E. coli Selection||Ampicillin (100 ug/mL)|
>OriGene ORF sequence for NM_000546 edited
>OriGene 5' read for NM_000546 unedited
>OriGene 3' read for NM_000546 unedited
|Insert Size||2500 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA|
|Product Components||The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2629 bp|
|RefSeq ORF||1182 bp|
|Protein Families||Druggable Genome, Stem cell - Pluripotency, Transcription Factors|
|Protein Pathways||Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway|
|Gene Summary||This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Transcript Variant: This variant (1) can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (a, also known as p53alpha) results from translation initiation at the upstream start codon. Both variants 1 and 2 encode isoform a, which is the longest isoform.
|The use of this cDNA Clones has been cited in the following citations:|
Length and secondary structure of the 5\' non-coding regions of mouse p53 mRNA transcripts - mouse as a model organism for p53 gene expression studies
,Szpotkowska, J;Swiatkowska, A;Ciesiołka, J;,
RNA Biol 2019
,PubMed ID 30518296
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition
,Yan, JK;Zhang, T;Dai, LN;Gu, BL;Zhu, J;Yan, WH;Cai, W;Wang, Y;,
FASEB J. 2018
,PubMed ID 30514107
HMGB1 represses the anti-cancer activity of sunitinib by governing TP53 autophagic degradation via its nucleus-to-cytoplasm transport
,Luo, P;Xu, Z;Li, G;Yan, H;Zhu, Y;Zhu, H;Ma, S;Yang, B;He, Q;,
,PubMed ID 30205729
Transcribed ultraconserved region 339 promotes carcinogenesis by modulating tumor suppressor microRNAs
,Vannini, I;Wise, PM;Challagundla, KB;Plousiou, M;Raffini, M;Bandini, E;Fanini, F;Paliaga, G;Crawford, M;Ferracin, M;Ivan, C;Fabris, L;Davuluri, RV;Guo, Z;Cortez, MA;Zhang, X;Chen, L;Zhang, S;Fernandez-Cymering, C;Han, L;Carloni, S;Salvi, S;Ling, H;Murtadha, M;Neviani, P;Gitlitz, BJ;Laird-Offringa, IA;Nana-Sinkam, P;Negrini, M;Liang, H;Amadori, D;Cimmino, A;Fabbri, M;Calin, GA;,
Nat Commun 2017
,PubMed ID 29180617
Molecular mechanism leading to SAHA-induced autophagy in tumor cells: evidence for a p53-dependent pathway
,Fröhlich, LF;Mrakovcic, M;Smole, C;Zatloukal, K;,
Cancer Cell Int. 2016
,PubMed ID 27601937
Cytosolic galectin-7 impairs p53 functions and induces chemoresistance in breast cancer cells
,Grosset, AA;Labrie, M;Gagné, D;Vladoiu, MC;Gaboury, L;Doucet, N;St-Pierre, Y;,
BMC Cancer Nov 2014
,PubMed ID 25367122
SCO2 Induces p53-Mediated Apoptosis by Thr845 Phosphorylation of ASK-1 and Dissociation of the ASK-1–Trx Complex
,Esha Madan, Rajan Gogna, Periannan Kuppusamy, Madan Bhatt, Abbas Ali Mahdi, and Uttam Pati,
Mol. Cell. Biol., Apr 2013; 33: 1285 - 1302.
,PubMed ID 23319048
miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53
,Francisco Navarro, David Gutman, Eti Meire, Mario Caceres, Isidore Rigoutsos, Zvi Bentwich, and Judy Lieberman,
Blood, Jul 2009; 10.1182/blood-2009-02-205062.
Interaction of Heterogeneous Nuclear Ribonucleoprotein C1/C2 with a Novel cis-Regulatory Element within p53 mRNA as a Response to Cytostatic Drug Treatment
,Kyle J. Christian, Matti A. Lang, and Françoise Raffalli-Mathieu,
Mol. Pharmacol., May 2008; 73: 1558 - 1567.
Macrophages Produce TGF-ß-Induced (ß-ig-h3) following Ingestion of Apoptotic Cells and Regulate MMP14 Levels and Collagen Turnover in Fibroblasts
,Zachary A. Cooper, Michael P. Gillmeister, Nevins W. Todd, and Sergei P. Atamas,
J. Cell Sci., Apr 2008; 121: 989 - 1001.
Interaction of hnRNPC1/C2 with a novel cis-regulatory element within p53 mRNA as a response to cytostatic drug treatment
,Kyle J Christian, Matti A. Lang, and Francoise Raffali-Mathieu,
Mol. Pharmacol., Feb 2008; 10.1124/mol.107.042507.
|cDNA Clone Resources|
|RC200003||TP53 (Myc-DDK-tagged)-Human tumor protein p53 (TP53), transcript variant 1||
|RG200003||TP53 (GFP-tagged) - Human tumor protein p53 (TP53), transcript variant 1||
|RC200003L1||Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, Myc-DDK-tagged||
|RC200003L2||Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, mGFP tagged||
|RC200003L3||Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, Myc-DDK-tagged||
|RC200003L4||Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, mGFP tagged||