p53 (TP53) (NM_000546) Human Untagged Clone

USD 540.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name p53 (TP53) (NM_000546) Human Untagged Clone
Type Human Untagged Clone
Tag Tag Free
Symbol TP53
Synonyms BCC7; BMFS5; LFS1; P53; TRP53
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000546 edited
ATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAGGAAACATTTTCA
GACCTATGGAAACTACTTCCTGAAAACAACGTTCTGTCCCCCTTGCCGTCCCAAGCAATG
GATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCA
GATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCAGCAGCTCCT
ACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAG
AAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAG
TCTGTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAACTGGCCAAGACC
TGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCCGCGCCATG
GCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCCCCCACCATGAG
CGCTGCTCAGATAGCGATGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAAT
TTGCGTGTGGAGTATTTGGATGACAGAAACACTTTTCGACATAGTGTGGTGGTGCCCTAT
GAGCCGCCTGAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGT
TCCTGCATGGGCGGCATGAACCGGAGGCCCATCCTCACCATCATCACACTGGAAGACTCC
AGTGGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGA
GACCGGCGCACAGAGGAAGAGAATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCC
CCAGGGAGCACTAAGCGAGCACTGCCCAACAACACCAGCTCCTCTCCCCAGCCAAAGAAG
AAACCACTGGATGGAGAATATTTCACCCTTCAGATCCGTGGGCGTGAGCGCTTCGAGATG
TTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATGCCCAGGCTGGGAAGGAGCCAGGG
GGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT
AAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGA
>OriGene 5' read for NM_000546 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTGCGTTCGGGC
TGGGAGCGTGCTTTCCACGACGGTGACACGCTTCCCTGGATTGGCAGCCAGACTGCCTTC
CGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAG
GAAACATTTTCAGACCTATGGAAACTACTTCCTGAAAACAACGTTCTGTCCCCCTTGCCG
TCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAA
GACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCA
CCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCT
GTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCT
GGGACAGCCAAGTCTGTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAA
CTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGC
GTCCGCGCCATGGCCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTG
CCCCCACCATGAGCGCTGCTCAGATAGCGATGGTCTGCCCCTCCTCAGCATCTTATCCGA
GTGGAAAGAAATTTGGCGTGTGGAGTATTTGGATGACAGAAAACACTTTTCCACATATGG
GNGTGGTCGCCCTAAGAGCCCCCCTGAGGTTGGGTCTGACTGTCCCACCATCCCTTCCAC
TAACTGGGAAACAGTTCTTGCTTGGCCGGGATGAACCGGAAGGCCCTCCTTACCTCATCA
CACTGGAAGACTCCGGGGAATTCACG
>OriGene 3' read for NM_000546 unedited
CCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGCAAAGTTTTATTG
TAAAATAAGAGATCGATATAAAAATGGGATATAAAAAGGGAGAAGGAGGGGAAGGGTGGG
GTGAAAATGCAGATGTGCTTGCAGAATGTAAAAGATGTTGACCCTTCCAGCTGGACGTGG
TGGCTCACAATTGTAATCCCAGCACTCTGGGAGGCTGAGACAGGTGGATCGCCTGAGCCC
AGGAGTTTGAGACCAGCCTGGGCAACACTGTGAGACCCCATCTCTACAAAACATGCAAAA
GTTGGCTGGCCATGGTGGCATGAACCTGTGGTCCCAGCTACTCCGGAGGCTGAGGCAGGA
CTGCTCGAGCCGGGGAGGCAAAGGCTGCAGTAAGCCAAGATCACGCCACTCCACTCCAGT
CTGGGCAACTAAGCGAGACCCAGTCTTCAAGTAATTAGACCCCTTTCATTCCACTCCAAA
TCCGATTTTGACCCTGAGCCTATAACAAGTCTTCGGTGGATCCCGCACCATCCTATACAA
AAACATGAAATTCTTCAAGGGTGTGGGATGCGGGTGACAATTTCCCTTTTACGGTTCCAA
CGGTTACCCAACAGGTTGGCCCACAATGGTTTGTGCTGGGCCCCTCATAACACTTGATAA
CTCCCCTCTTACTTAACCACGTTTCACAAATACGTATATACTCCTCCCACCCCTCTTCAC
ACCATCGGCTATGGGCACCCCCCCTCCGTCAAGGCGCGTCTACTCCCTCCCCCCACCTCT
CACATAACAGTCTCTACACCCGAGTCTCACTCCTCTTCTATTTATTTCCTCCTCCACCCC
GCTCTCCTGCCCGTCTGCTTCCCCATTCTACCCCTCCCCTCCCTCAATTATTACCCCTCT
CTCTCACTCTATAACTCCCAAAGTGCCATGCCACACATTTATCTTCTCCCGTTCTACCCG
CCACAGTTCACTCTCTCCGTATAATCGCATTCTAAACATCCGTCGCTCACCCCC
Restriction Sites NotI-NotI     
ACCN NM_000546
Insert Size 2500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000546.2, NP_000537.2
RefSeq Size 2629 bp
RefSeq ORF 1182 bp
Locus ID 7157
Cytogenetics 17p13.1
Domains P53
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway
Gene Summary This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Transcript Variant: This variant (1) can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (a, also known as p53alpha) results from translation initiation at the upstream start codon. Both variants 1 and 2 encode isoform a, which is the longest isoform.

Citations (12)

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