p53(TP53) (NM_000546) Human Untagged Clone
TP53 (untagged)-Human tumor protein p53 (TP53), transcript variant 1
|Product Name||p53(TP53) (NM_000546) Human Untagged Clone|
|Synonyms||BCC7; LFS1; P53; TRP53|
Fully Sequenced ORF
>OriGene ORF sequence for NM_000546 edited
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000546 unedited
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000546 unedited
|ORF Size||1182 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA|
|Product Components||The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.|
|Protein Families||Stem cell - Pluripotency, Transcription Factors, Druggable Genome|
|Protein Pathways||MAPK signaling pathway, Cell cycle, p53 signaling pathway, Apoptosis, Wnt signaling pathway, Neurotrophin signaling pathway, Amyotrophic lateral sclerosis (ALS), Huntington's disease, Pathways in cancer, Colorectal cancer, Pancreatic cancer, Endometrial cancer, Glioma, Prostate cancer, Thyroid cancer, Basal cell carcinoma, Melanoma, Bladder cancer, Chronic myeloid leukemia, Small cell lung cancer, Non-small cell lung cancer|
|Gene Summary||This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Transcript Variant: This variant (1) can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (a, also known as p53alpha) results from translation initiation at the upstream start codon. Both variants 1 and 2 encode isoform a, which is the longest isoform.
|The use of this cDNA Clones has been cited in the following citations:|
Molecular mechanism leading to SAHA-induced autophagy in tumor cells: evidence for a p53-dependent pathway
,Fröhlich, LF;Mrakovcic, M;Smole, C;Zatloukal, K;,
Cancer Cell Int. 2016,PubMed ID 27601937
Cytosolic galectin-7 impairs p53 functions and induces chemoresistance in breast cancer cells
,Grosset, AA;Labrie, M;Gagné, D;Vladoiu, MC;Gaboury, L;Doucet, N;St-Pierre, Y;,
BMC Cancer Nov 2014,PubMed ID 25367122
SCO2 Induces p53-Mediated Apoptosis by Thr845 Phosphorylation of ASK-1 and Dissociation of the ASK-1–Trx Complex
,Esha Madan, Rajan Gogna, Periannan Kuppusamy, Madan Bhatt, Abbas Ali Mahdi, and Uttam Pati,
Mol. Cell. Biol., Apr 2013; 33: 1285 - 1302.,PubMed ID 23319048
miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53
,Francisco Navarro, David Gutman, Eti Meire, Mario Caceres, Isidore Rigoutsos, Zvi Bentwich, and Judy Lieberman,
Blood, Jul 2009; 10.1182/blood-2009-02-205062.
Interaction of Heterogeneous Nuclear Ribonucleoprotein C1/C2 with a Novel cis-Regulatory Element within p53 mRNA as a Response to Cytostatic Drug Treatment
,Kyle J. Christian, Matti A. Lang, and Françoise Raffalli-Mathieu,
Mol. Pharmacol., May 2008; 73: 1558 - 1567.
Macrophages Produce TGF-ß-Induced (ß-ig-h3) following Ingestion of Apoptotic Cells and Regulate MMP14 Levels and Collagen Turnover in Fibroblasts
,Zachary A. Cooper, Michael P. Gillmeister, Nevins W. Todd, and Sergei P. Atamas,
J. Cell Sci., Apr 2008; 121: 989 - 1001.
Interaction of hnRNPC1/C2 with a novel cis-regulatory element within p53 mRNA as a response to cytostatic drug treatment
,Kyle J Christian, Matti A. Lang, and Francoise Raffali-Mathieu,
Mol. Pharmacol., Feb 2008; 10.1124/mol.107.042507.
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