ADAR1 (ADAR) (NM_001111) Human Untagged Clone

CAT#: SC119438

ADAR (untagged)-Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAR1
• Synonyms: ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119438 sequence for NM_001111 edited (data generated by NextGen Sequencing)
  ATGAATCCGCGGCAGGGGTATTCCCTCAGCGGATACTACACCCATCCATTTCAAGGCTAT
  GAGCACAGACAGCTCAGATACCAGCAGCCTGGGCCAGGATCTTCCCCCAGTAGTTTCCTG
  CTTAAGCAAATAGAATTTCTCAAGGGGCAGCTCCCAGAAGCACCGGTGATTGGAAAGCAG
  ACACCGTCACTGCCACCTTCCCTCCCAGGACTCCGGCCAAGGTTTCCAGTACTACTTGCC
  TCCAGTACCAGAGGCAGGCAAGTGGACATCAGGGGTGTCCCCAGGGGCGTGCATCTCGGA
  AGTCAGGGGCTCCAGAGAGGGTTCCAGCATCCTTCACCACGTGGCAGGAGTCTGCCACAG
  AGAGGTGTTGATTGCCTTTCCTCACATTTCCAGGAACTGAGTATCTACCAAGATCAGGAA
  CAAAGGATCTTAAAGTTCCTGGAAGAGCTTGGGGAAGGGAAGGCCACCACAGCACATGAT
  CTGTCTGGGAAACTTGGGACTCCGAAGAAAGAAATCAATCGAGTTTTATACTCCCTGGCA
  AAGAAGGGCAAGCTACAGAAAGAGGCAGGAACACCCCCTTTGTGGAAAATCGCGGTCTCC
  ACTCAGGCTTGGAACCAGCACAGCGGAGTGGTAAGACCAGACGGTCATAGCCAAGGAGCC
  CCAAACTCAGACCCGAGTTTGGAACCGGAAGACAGAAACTCCACATCTGTCTCAGAAGAT
  CTTCTTGAGCCTTTTATTGCAGTCTCAGCTCAGGCTTGGAACCAGCACAGCGGAGTGGTA
  AGACCAGACAGTCATAGCCAAGGATCCCCAAACTCAGACCCAGGTTTGGAACCTGAAGAC
  AGCAACTCCACATCTGCCTTGGAAGATCCTCTTGAGTTTTTAGACATGGCCGAGATCAAG
  GAGAAAATCTGCGACTATCTCTTCAATGTGTCTGACTCCTCTGCCCTGAATTTGGCTAAA
  AATATTGGCCTTACCAAGGCCCGAGATATAAATGCTGTGCTAATTGACATGGAAAGGCAG
  GGGGATGTCTATAGACAAGGGACAACCCCTCCCATATGGCATTTGACAGACAAGAAGCGA
  GAGAGGATGCAAATCAAGAGAAATACGAACAGTGTTCCTGAAACCGCTCCAGCTGCAATC
  CCTGAGACCARAAGAAACGCAGAGTTCCTCACCTGTAATATACCCACATCAAATGCCTCA
  AATAACATGGTAACCACAGAAAAAGTGGAGAATGGGCAGGAACCTGTCATAAAGTTAGAA
  AACAGGCAAGAGGCCAGACCAGAACCAGCAAGACTGAAACCACCTGTTCATTACAATGGC
  CCCTCAAAAGCAGGGTATGTTGACTTTGAAAATGGCCAGTGGGCCACAGATGACATCCCA
  GATGACTTGAATAGTATCCGCGCAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCC
  TCCTTCTACAGTCATGGCTTGCCACGGTGTTCACCCTACAAGAAACTGACAGAGTGCCAG
  CTGAAGAACCCCATCAGCGGGCTGTTAGAATATGCCCAGTTCGCTAGTCAAACCTGTGAG
  TTCAACATGATAGAGCAGAGTGGACCACCCCATGAACCTCGATTTAAATTCCAGGTTGTC
  ATCAATGGCCGAGAGTTTCCCCCAGCTGAAGCTGGAAGCAAGAAAGTGGCCAAGCAGGAT
  GCAGCTATGAAAGCCATGACAATTCTGCTAGAGGAAGCCAAAGCCAAGGACAGTGGAAAA
  TCAGAAGAATCATCCCACTATTCCACAGAGAAAGAATCAGAGAAGACTGCAGAGTCCCAG
  ACCCCCACCCCTTCAGCCACATCCTTCTTTTCTGGGAAGAGCCCCGTCACCACACTGCTT
  GAGTGTATGCACAAATTGGGGAACTCCTGCGAATTCCGTCTCCTGTCCAAAGAAGGCCCT
  GCCCATGAACCCAAGTTCCAATACTGTGTTGCAGTGGGAGCCCAAACTTTCCCCAGTGTG
  AGTGCTCCCAGCAAGAAAGTGGCAAAGCAGATGGCCGCAGAGGAAGCCATGAAGGCCCTG
  CATGGGGAGGCGACCAACTCCATGGCTTCTGATAACCAGCCTGAAGGTATGATCTCAGAG
  TCACTTGATAACTTGGAATCCATGATGCCCAACAAGGTCAGGAAGATTGGCGAGCTCGTG
  AGATACCTGAACACCAACCCTGTGGGTGGCCTTTTGGAGTACGCCCGCTCCCATGGCTTT
  GCTGCTGAATTCAAGTTGGTCGACCAGTCCGGACCTCCTCACGAGCCCAAGTTCGTTTAC
  CAAGCAAAAGTTGGGGGTCGCTGGTTCCCAGCCGTCTGCGCACACAGCAAGAAGCAAGGC
  AAGCAGGAAGCAGCAGATGCGGCTCTCCGTGTCTTGATTGGGGAGAACGAGAAGGCAGAA
  CGCATGGGTTTCACAGAGGTAACCCCAGTGACAGGGGCCAGTCTCAGAAGAACTATGCTC
  CTCCTCTCAAGGTCCCCAGAAGCACAGCCAAAGACACTCCCTCTCACTGGCAGCACCTTC
  CATGACCAGATAGCCATGCTGAGCCACCGGTGCTTCAACACTCTGACTAACAGCTTCCAG
  CCCTCCTTGCTCGGCCGCAAGATTCTGGCCGCCATCATTATGAAAAAAGACTCTGAGGAC
  ATGGGTGTCGTCGTCAGCTTGGGAACAGGGAATCGCTGTGTGAAAGGAGATTCTCTCAGC
  CTAAAAGGAGAAACTGTCAATGACTGCCATGCAGAAATAATCTCCCGGAGAGGCTTCATC
  AGGTTTCTCTACAGTGAGTTAATGAAATACAACTCCCAGACTGCGAAGGATAGTATATTT
  GAACCTGCTAAGGGAGGAGAAAAGCTCCAAATAAAAAAGACTGTGTCATTCCATCTGTAT
  ATCAGCACTGCTCCGTGTGGAGATGGCGCCCTCTTTGACAAGTCCTGCAGCGACCGTGCT
  ATGGAAAGCACAGAATCCCGCCACTACCCTGTCTTCGAGAATCCCAAACAAGGAAAGCTC
  CGCACCAAGGTGGAGAACGGAGAAGGCACAATCCCTGTGGAATCCAGTGACATTGTGCCT
  ACGTGGGATGGCATTCGGCTCGGGGAGAGACTCCGTACCATGTCCTGTAGTGACAAAATC
  CTACGCTGGAACGTGCTGGGCCTGCAAGGGGCACTGTTGACCCACTTCCTGCAGCCCATT
  TATCTCAAATCTGTCACATTGGGTTACCTTTTCAGCCAAGGGCATCTGACCCGTGCTATT
  TGCTGTCGTGTGACAAGAGATGGGAGTGCATTTGAGGATGGACTACGACATCCCTTTATT
  GTCAACCACCCCAAGGTTGGCAGAGTCAGCATATATGATTCCAAAAGGCAATCCGGGAAG
  ACTAAGGAGACAAGCGTCAACTGGTGTCTGGCTGATGGCTATGACCTGGAGATCCTGGAC
  GGTACCAGAGGCACTGTGGATGGGCCACGGAATGAATTGTCCCGGGTCTCCAAAAAGAAC
  ATTTTTCTTCTATTTAAGAAGCTCTGCTCCTTCCGTTACCGCAGGGATCTACTGAGACTC
  TCCTATGGTGAGGCCAAGAAAGCTGCCCGTGACTACGAGACGGCCAAGAACTACTTCAAA
  AAAGGCCTGAAGGATATGGGCTATGGGAACTGGATTAGCAAACCCCAGGAGGAAAAGAAC
  TTTTATCTCTGCCCAGTATAG
  
  Clone variation with respect to NM_001111.4
  78 g=>a;1151 a=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001111 unedited
  TAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
  AATTCGGCACGAGGCTTGGGCCCGGCGCGCAGACCCGCGGAGTTTCCCGTGCCGACGCCC
  CGGGGCCACTTCCAGTGCGGAGTAGCGGAGGCGTGGGGGCCTCGAGGGGCTGGCGCGGCC
  CAGCGGTCGGGCCAGGGTCGTGCCGCCGGCGGGTCGGGCCGGGCAATGCCTCGCGGGCGC
  AATGAATCCGCGGCAGGGGTATTCCCTCAGCGGATACTACACCCATCCATTTCAAGGCTA
  TGAGCACAGACAGCTCAGATACCAGCAGCCTGGGCCAGGATCTTCCCCCAGTAGTTTCCT
  GCTTAAGCAAATAGAATTTCTCAAGGGGCAGCTCCCAGAAGCACCGGTGATTGGAAAGCA
  GACACCGTCACTGCCACCTTCCCTCCCAGGACTCCGGCCAAGGTTTCCAGTACTACTTGC
  CTCCAGTACCAGAGGCAGGNCAGTGGACATCAGGGGTGTCCCCAGGGGCGTGCATCTCGG
  AAGTCAGGGGCTCCAGAGAGGGTTNCAGCATCCTTCACCACGTGGCAGGAGTCTGCCCAG
  AGAGGTGTTGATTGCCTTTTCTCACATTNCCAGGACTGAGTATCTACAAGATCAGGACAN
  AGGATCCTTAAGTTCCTGGAANAGCCTGGGGAAAGGAAGGGCANCACCGCACATGATCTG
  TCTGGGAAACTTGGGACTCCGAGAAGAAGTCAATCGAGTTTATACTCCTGGCAAAGAAGG
  CAGCTCAGAAGAGGCAGAACCCCCTTGTGAAAAAACGGGCTCCCTCAGCTTGGACCCAGC
  GGGGTGGGTAAGACCAACGGCTAGCCAGGGCCCACTAACCCCCGTTTGGACCGGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001111 unedited
  GGTTCCACTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTCAA
  ATTCTTGTTTTTTTTTTATTTATGAGGAATGTATATTGTTAAGTCACTGTTATCAAGGGA
  CACATAAAAGCAGAATCATAAAACTGGACTGCAGCCATCATCACAGTACCCGAGTCTATG
  CTTGGGGGTCTTCCTCACTCTGCTCTTGGAGTCACAACCAAGACTCCAAAAAACCAACGA
  AGTCCCTTCAATGTGAGTAAAGGAAACAGCTTCAAGCACTGACAATTTTTACAAGTGATT
  ATTCAAGGAATGCACAGTAGCCACAGTTCAGACAAATCAACTACAAAAATCATTAAAAAG
  AAGAGTAGGGAGGGTCACTGCTTATTAAAAACAAAAGACTGGACAAAGAAAGTGAAGATA
  ACAGTGGGCAGAAGCTCTGCCTCCTCTGTCCTGTGTGACTCACAGGGGCATGGGGGCATG
  GGGGCCCAAGGATGTGCGATCTGACACGGCTCGTTTCCAACAGATGCGTGGAATTCAAGG
  ATGCCTCGCTCTCTTCCTACAACAGAAACTGAATACTACAGGCTTGCTAAATAAAATCTT
  GGGGGGGAAGGGGGGGTTCTGAACTTGTGAAAACCCGTGTTCCCCGTTAAAAAAAAGCAA
  CTGGCCCAAACTCTGCTGGAAAACTCATGCCCCAGATGGACCCAAAGGCCAAACACCCGA
  GGACTCAGCGGGGACAACCCTGTGCCCTAAAAAAAAAGGGGTGCCCCTCCTTCTTATGCG
  GAAAACCCAAAACCTTTTTGCCCGGAATTGGGTGCCCAAAATGTTTTATGTTGCCCCCCA
  TAAACCCAAGCCGGGGAGAGGGCTTTTTCACTTTTGGAAAAGGGGCCCTTTTCTACCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001111
• Insert Size: 7000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111.3, NP_001102.2
• RefSeq Size: 6640 bp
• RefSeq ORF: 3681 bp
• Locus ID: 103
• UniProt ID: P55265
• Cytogenetics: 1q21.3
• Domains: z-alpha, DSRM, A_deamin
• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway
• Gene Summary: This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) encodes isoform (a, also referred to as ADAR-a).