BMX (NM_001721) Human Untagged Clone

SKU SC119060

BMX (untagged)-Human BMX non-receptor tyrosine kinase (BMX), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: BMX
• Synonyms: ETK; PSCTK2; PSCTK3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001721 edited; GGGCGGCCGCGAATTCGGCACGAGGCTGCAACAGCAGACCAAGCACCGCGGCGGACCCAG; GCAAGCACGGAACAAGCTGAGACGGATGATAATATGGATACAAAATCTATTCTAGAAGAA; CTTCTTCTCAAAAGATCACAGCAAAAGAAGAAAATGTCACCAAATAATTACAAAGAACGG; CTTTTTGTTTTGACCAAAACAAACCTTTCCTACTATGAATATGACAAAATGAAAAGGGGC; AGCAGAAAAGGATCCATTGAAATTAAGAAAATCAGATGTGTGGAGAAAGTAAATCTCGAG; GAGCAGACGCCTGTAGAGAGACAGTACCCATTTCAGATTGTCTATAAAGATGGGCTTCTC; TATGTCTATGCATCAAATGAAGAGAGCCGAAGTCAGTGGTTGAAAGCATTACAAAAAGAG; ATAAGGGGTAACCCCCACCTGCTGGTCAAGTACCATAGTGGGTTCTTCGTGGACGGGAAG; TTCCTGTGTTGCCAGCAGAGCTGTAAAGCAGCCCCAGGATGTACCCTCTGGGAAGCATAT; GCTAATCTGCATACTGCAGTCAATGAAGAGAAACACAGAGTTCCCACCTTCCCAGACAGA; GTGCTGAAGATACCTCGGGCAGTTCCTGTTCTCAAAATGGATGCACCATCTTCAAGTACC; ACTCTAGCCCAATATGACAACGAATCAAAGAAAAACTATGGCTCCCAGCCACCATCTTCA; AGTACCAGTCTAGCGCAATATGACAGCAACTCAAAGAAAATCTATGGCTCCCAGCCAAAC; TTCAACATGCAGTATATTCCAAGGGAAGACTTCCCTGACTGGTGGCAAGTAAGAAAACTG; AAAAGTAGCAGCAGCAGTGAAGATGTTGCAAGCAGTAACCAAAAAGAAAGAAATGTGAAT; CACACCACCTCAAAGATTTCATGGGAATTCCCTGAGTCAAGTTCATCTGAAGAAGAGGAA; AACCTGGATGATTATGACTGGTTTGCTGGTAACATCTCCAGATCACAATCTGAACAGTTA; CTCAGACAAAAGGGAAAAGAAGGAGCATTTATGGTTAGAAATTCGAGCCAAGTGGGAATG; TACACAGTGTCCTTATTTAGTAAGGCTGTGAATGATAAAAAAGGAACTGTCAAACATTAC; CACGTGCATACAAATGCTGAGAACAAATTATACCTGGCAGAAAACTACTGTTTTGATTCC; ATTCCAAAGCTTATTCATTATCATCAACACAATTCAGCAGGCATGATCACACGGCTCCGC; CACCCTGTGTCAACAAAGGCCAACAAGGTCCCCGACTCTGTGTCCCTGGGAAATGGAATC; TGGGAACTGAAAAGAGAAGAGATTACCTTGTTGAAGGAGCTGGGAAGTGGCCAGTTTGGA; GTGGTCCAGCTGGGCAAGTGGAAGGGGCAGTATGATGTTGCTGTTAAGATGATCAAGGAG; GGCTCCATGTCAGAAGATGAATTCTTTCAGGAGGCCCAGACTATGATGAAACTCAGCCAT; CCCAAGCTGGTTAAATTCTATGGAGTGTGTTCAAAGGAATACCCCATATACATAGTGACT; GAATATATAAGCAATGGCTGCTTGCTGAATTACCTGAGGAGTCACGGAAAAGGACTTGAA; CCTTCCCAGCTCTTAGAAATGTGCTACGATGTCTGTGAAGGCATGGCCTTCTTGGAGAGT; CACCAATTCATACACCGGGACTTGGCTGCTCGTAACTGCTTGGTGGACAGAGATCTCTGT; GTGAAAGTATCTGACTTTGGAATGACAAGGTATGTTCTTGATGACCAGTATGTCAGTTCA; GTCGGAACAAAGTTTCCAGTCAAGTGGTCAGCTCCAGAGGTGTTTCATTACTTCAAATAC; AGCAGCAAGTCAGACGTATGGGCATTTGGGATCCTGATGTGGGAGGTGTTCAGCCTGGGG; AAGCAGCCCTATGACTTGTATGACAACTCCCAGGTGGTTCTGAAGGTCTCCCAGGGCCAC; AGGCTTTACCGGCCCCACCTGGCATCGGACACCATCTACCAGATCATGTACAGCTGCTGG; CACGAGCTTCCAGAAAAGCGTCCCACATTTCAGCAACTCCTGTCTTCCATTGAACCACTT; CGGGAAAAAGACAAGCATTGAAGAAGAAATTAGGAGTGCTGATAAGAATGAATATAGATG; CTGGCCAGCATTTTCATTCATTTTAAGGAAAGTAGCAAGGCATAATGTAATTTAGCTAGT; TTTTAATAGTGTTCTCTGTATTGTCTATTATTTAGAAATGAACAAGGCAGGAAACAAAAG; ATTCCCTTGAAATTTAGATCAAATTAGTAATTTTGTTTATGCTGCTCCTGATATAACACT; TTCCAGCCTATAGCAGAAGCACATTTTCAGACTGCAATATAGAGACTGTGTTCATGTGTA; AAGACTGAGCAGAACTGAAAAATTACTTATTGGATATTCATTCTTTTCTTTATATTGTCA; TTGTCACAACAATTAAATATACTACCAAGTACAGAAAAAAAAAAAAAAAAAACTCGACTC; TAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGAC; CCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001721 unedited; TCCAGCAATTTGTAATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTGCAA; CAGCAGACCAAGCACCGCGGCGGACCCAGGCAAGCACGGAACAAGCTGAGACGGATGATA; ATATGGATACAAAATCTATTCTAGAAGAACTTCTTCTCAAAAGATCACAGCAAAAGAAGA; AAATGTCACCAAATAATTACAAAGAACGGCTTTTTGTTTTGACCAAAACAAACCTTTCCT; ACTATGAATATGACAAAATGAAAAGGGGCAGCAGAAAAGGATCCATTGAAATTAAGAAAA; TCAGATGTGTGGAGAAAGTAAATCTCGAGGAGCAGACGCCTGTAGAGAGACAGTACCCAT; TTCAGATTGTCTATAAAGATGGGCTTCTCTATGTCTATGCATCAAATGAAGAGAGCCGAA; GTCAGTGGTTGAAAGCATTACAAAAAGAGATAAGGGGTAACCCCCACCTGCTGGTCAAGT; ACCATAGTGGGTTCTTCGTGGACGGGAAGTTCCTGTGTTGCCAGCAGAGCTGTAAAGCAG; CCCCAGGATGTACCCTCTGGGAAGCATATGCTAATCTGCATACTGCAGTCAATGAAGAGA; AACACAGAGTTCCCACCTTCCCAGACAGAGTGCTGAAGATACCTCGGGCAGTTCCTGTTC; TCAAAATGGATGCACCATCTTCAAGTACCACTCTAGCCCAATATGACAACGAATCAAAGA; AAAACTATGGCTCCCAGNCACCATCTTCAAGTACCAGTCTAGCGCAATATGACAGCAACT; CAAAGAAAATCTATGGCTTCCAGCCAAACTTCACATGCAGTATATTNCAGGGGAAGACCT; CCCTGACTGGCGGCAAGTAAGAAAACTGAAAAGCATCATCACCAGTGAAGATGTTGCAGC; AGTTACCAAAAAGAAAAAAAGGGATCCCACCCCTCTAAGATTCATGGGGATCCCTGAGTN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001721 unedited; ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTGTACTTGGTAGTAT; ATTTAATTGTTGTGACAATGACAATATAAAGAAAAGAATGAATATCCAATAAGTAATTTT; TCAGTTCTGCTCAGTCTTTACACATGAACACAGTCTCTATATTGCAGTCTGAAAATGTGC; TTCTGCTATAGGCTGGAAAGTGTTATATCAGGAGCAGCATAAACAAAATTACTAATTTGA; TCTAAATTTCAAGGGAATCTTTTGTTTCCTGCCTTGTTCATTTCTAAATAATAGACAATA; CAGAGAACACTATTAAAAACTAGCTAAATTACATTATGCCTTGCTACTTTCCTTAAAATG; AATGAAAATGCTGGCCAGCATCTATATTCATTCTTATCAGCACTCCTAATTTCTTCTTCA; ATGCTTGTCTTTTTCCCGAAGTGGTTCAATGGAAGACAGGAGTTGCTGAAATGTGGGACG; CTTTTCTGGAAGCTCGTGCCAGCAGCTGTACATGATCTGGTAGATGGTGTCCGATGCCAG; GTGGGGCCGGTAAAGCCTGTGGCCCTGGGAGACCTTCAGAACCACCTGGGAGTTGTCATA; CAAGTCATAGGGCTGCTTCCCCAGGCTGAACACCTCCCACATCAGGATCCCAAATGCCCA; TACGTCTGACTTGCTGCTGTATTTGAAGTAATGAAACACCTCTGGAGCTGACCACTTGAC; TGGAAACTNTGTTCCGACTGAACTGACATACTGGTCATCAAGAACATACCTTGTCATTCC; AAAGTCAGATACTTCACACAGAGATCTCTGTCCACCAGCAGTTACGAGCAGCCAAGTCCC; GGTGTATGAATTGGTGACTCTCCAAGAAGCCATGCCTTCACAGAATCGTANCACATTCTA; GAGCTGGAAGGNNTCAGTCCTTTCCGGGCTCCTCAGTAATCAGAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_001721
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001721.4, NP_001712.1
• RefSeq Size: 2514 bp
• RefSeq ORF: 2028 bp
• Locus ID: 660
• UniProt ID: P51813
• Cytogenetics: Xp22.2
• Domains: BTK, PH, pkinase, SH2, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Summary: This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (2) has an alternate 5' non-coding exon compared to variant 1. Variants 1 and 2 encode the same isoform (1).