CYC1 (NM_001916) Human Untagged Clone

SKU
SC118940
CYC1 (untagged)-Human cytochrome c-1 (CYC1), nuclear gene encoding mitochondrial protein
$300.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CYC1
Synonyms MC3DN6; UQCR4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118940 sequence for NM_001916 edited (data generated by NextGen Sequencing)
ATGGCGGCAGCTGCGGCTTCGCTTCGCGGGGTAGTGTTGGGCCCGCGGGGCGCGGGGCTC
CCGGGCGCGCGTGCCCGGGGTCTGCTGTGCAGCGCGCGGCCCGGGCAGCTCCCGCTACGG
ACACCTCAGGCAGTGGCCTTGTCGTCGAAGTCTGGCCTTTCCCGAGGCCGGAAAGTGATG
CTGTCAGCGCTGGGCATGCTGGCGGCAGGGGGTGCGGGGCTGGCCGTGGCTCTGCATTCG
GCTGTGAGTGCCAGTGACCTGGAGCTGCACCCCCCCAGCTATCCGTGGTCTCACCGTGGC
CTCCTCTCTTCCTTGGACCACACCAGCATCCGGAGGGGTTTCCAGGTATATAAGCAGGTG
TGCGCCTCCTGCCACAGCATGGACTTCGTGGCCTACCGCCACCTGGTGGGCGTGTGCTAC
ACGGAGGATGAAGCTAAGGAGCTGGCTGCGGAGGTGGAGGTTCAAGACGGCCCCAATGAA
GATGGGGAGATGTTCATGCGGCCAGGGAAGCTGTTCGACTATTTCCCAAAACCATACCCC
AACAGTGAGGCTGCTCGAGCTGCCAACAACGGAGCATTGCCCCCTGACCTCAGCTACATC
GTGCGAGCTAGGCATGGTGGTGAGGACTACGTCTTCTCCCTGCTCACGGGCTACTGCGAG
CCACCCACCGGGGTGTCACTGCGGGAAGGTCTCTACTTCAACCCCTACTTTCCTGGCCAG
GCCATTGCCATGGCCCCTCCCATCTACACAGATGTCTTAGAGTTTGACGATGGCACCCCA
GCTACCATGTCCCAGATAGCCAAGGATGTGTGCACCTTCCTGCGCTGGGCATCTGAGCCA
GAGCACGACCATCGAAAACGCATGGGGCTCAAGATGTTGATGATGATGGCTCTGCTGGTG
CCCCTGGTCTACACCATAAAGCGGCACAAGTGGTCAGTCCTGAAGAGTCGGAAGCTGGCA
TATCGGCCGCCCAAGTGA

Clone variation with respect to NM_001916.3
99 t=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001916 unedited
CCGTCGGATTTGTATACGATTCACTATAGGCGGCCGCGAATTCGCACGAGGCGCGGAGGA
GGCCAAGATGGCGGCAGCTGCGGCTTCGCTTCGCGGGGTAGTGTTGGGCCCGCGGGGCGC
GGGGCTCCCGGGCGCGCGTGCCCGGGGTCTGCTGTGCAGCGCGCGGCCCGGGCAGCTCCC
GCTACGGACACCTCAGGCAGTGGCCTTGTCGTCGAAGTCTGGCCTTTCCCGAGGCCGGAA
AGTGATGCTGTCAGCGCTGGGCATGCTGGCGGCAGGGGGTGCGGGGCTGGCCGTGGCTCT
GCATTCGGCTGTGAGTGCCAGTGACCTGGAGCTGCACCCCCCCAGCTATCCGTGGTCTCA
CCGTGGCCTCCTCTCTTCCTTGGACCACACCAGCATCCGGAGGGGTTTCCAGGTATATAA
GCAGGTGTGCGCCTCCTGCCACAGCATGGACTTCGTGGCCTACCGCCACCTGGTGGGCGT
GTGCTACACGGAGGATGAAGCTAAGGAGCTGGCTGCGGAGGTGGAGGTTCAAGACGGCCC
CAATGAAGATGGGGAGATGTTCATGCGGCCAGGGAAGCTGTTCGACTATTTCCCAAAACC
ATACCCCAACAGTGAGGCTGCTCGAGCTGCCAACAACGGAGCATTGCCCCCTGACCTCAG
CTACATCGTGCGAGCTAGGCATGGTGGTGAGGACTACGTCTTCTCCCTGCTCACGGGCTA
CTGCGAGCCACCCACCGNNGTGTCACTGCNGGGAAGGTCTCTACTTCAACCCCTACTTTC
CTGGCCAGGCCATTGCCATGGCCCCTCCCACTACACAGATGCCTAAAAGTTTGACGATGG
CACCCCAGCTACCATGTCNCCAGATAGCCAAGTATGTGTGCACCTTCCTGCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001916 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACACTTGAAAAAATTAATTTATT
CCCATGATGGGGGCGTGAAGGAGGGCCCAAAGCTAGAGCCTGGTCTCCTGCCCCCTTGCC
TCTTCTTCCAGATGAAGAGAGGCTTAGCTGAGGCCTGAACAGGCCTGGGATGGCTCTTGG
GCTTGAGGGCCTGTTCTGGCAGGATGGCAAGCAGACACTGGACAGGGTCACTTGGGCGGC
CGATATGCCAGCTTCCGACTCTTCAGGACTGACCACTTGTGCCGCTTTATGGTGTAGACC
AGGGGCACCAGCAGAGCCATCATCATCAACATCTTGAGCCCCATGCGTTTTCGATGGTCG
TGCTCTGGCTCAGATGCCCAGCGCAGGAAGGTGCACACATCCTTGGCTATCTGGGACATG
GTAGCTGGGGTGCCATCGTCAAACTCTAAGACATCTGTGTAGATGGGAGGGGCCATGGCA
ATGGCCTGGCCAGGAAAGTAGGGGTTGAAGTANAGACCTTCCCGCAGTGACACCCCGGTG
GGTGGCTCGCAGTANCCCGTGAGCAGGGAGAAGACGTAGTCCTCACCACCATGCCTAGCT
CGCACGATGTANCTGAGGTCAGGGGGGCATGCTCCGTTGTTGGCAGCTCGAGCAGCCTCA
CTGTTGGGGGTATGGGTTTGGAAAATAATCGACAGCTTCCCTGGCCCATGAACATCTTCC
CATCTTCATGGGGGCCGGCTTGAACCTTCACCTCCCGAGCCAGGTCCTTTAATTTATCCC
TCGGGGACCCCCCCCCACCCAGGTGGGGGGAAGCCACCAAATCCTTGCGTGGGAAGGGGG
CCCCCCCCTGGTTTATACCCTGGGAACCCCTCCGGAGCCCGGGTGGGCCAAGGGAAAAGG
GGGGCCCGGGGACACCCCATTTTTGGGGGGGGGCA
Restriction Sites ECoRI-NOT
ACCN NM_001916
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001916.2, NP_001907.2
RefSeq Size 1273 bp
RefSeq ORF 978 bp
Locus ID 1537
UniProt ID P08574
Cytogenetics 8q24.3
Domains Cytochrome_C1
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Summary This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6. [provided by RefSeq, Dec 2013]
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Citations

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