GFAP (NM_002055) Human Untagged Clone

SKU SC118873

GFAP (untagged)-Human glial fibrillary acidic protein (GFAP), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GFAP
• Synonyms: ALXDRD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118873 sequence for NM_002055 edited (data generated by NextGen Sequencing); ATGGAGAGGAGACGCATCACCTCCGCTGCTCGCCGCTCCTACGTCTCCTCAGGGGAGATG; ATGGTGGGGGGCCTGGCTCCTGGCCGCCGTCTGGGTCCTGGCACCCGCCTCTCCCTGGCT; CGAATGCCCCCTCCACTCCCAACCCGGGTGGATTTCTCCCTGGCTGGGGCACTCAATGCT; GGCTTCAAGGAGACCCGGGCCAGTGAGCGGGCAGAGATGATGGAGCTCAATGACCGCTTT; GCCAGCTACATCGAGAAGGTTCGCTTCCTGGAACAGCAAAACAAGGCGCTGGCTGCTGAG; CTGAACCAGCTGCGGGCCAAGGAGCCCACCAAGCTGGCAGACGTCTACCAGGCTGAGCTG; CGAGAGCTGCGGCTGCGGCTCGATCAACTCACCGCCAACAGCGCCCGGCTGGAGGTTGAG; AGGGACAATCTGGCACAGGACCTGGCCACTGTGAGGCAGAAGCTCCAGGATGAAACCAAC; CTGAGGCTGGAAGCCGAGAACAACCTGGCTGCCTATAGACAGGAAGCAGATGAAGCCACC; CTGGCCCGTCTGGATCTGGAGAGGAAGATTGAGTCGCTGGAGGAGGAGATCCGGTTCTTG; AGGAAGATCCACGAGGAGGAGGTTCGGGAACTCCAGGAGCAGCTGGCCCGACAGCAGGTC; CATGTGGAGCTTGACGTGGCCAAGCCAGACCTCACCGCAGCCCTGAAAGAGATCCGCACG; CAGTATGAGGCAATGGCGTCCAGCAACATGCATGAAGCCGAAGAGTGGTACCGCTCCAAG; TTTGCAGACCTGACAGACGCTGCTGCCCGCAACGCGGAGCTGCTCCGCCAGGCCAAGCAC; GAAGCCAACGACTACCGGCGCCAGTTGCAGTCCTTGACCTGCGACCTGGAGTCTCTGCGC; GGCACGAACGAGTCCCTGGAGAGGCAGATGCGCGAGCAGGAGGAGCGGCACGTGCGGGAG; GCGGCCAGTTATCAGGAGGCGCTGGCGCGGCTGGAGGAAGAGGGGCAGAGCCTCAAGGAC; GAGATGGCCCGCCACTTGCAGGAGTACCAGGACCTGCTCAATGTCAAGCTGGCCCTGGAC; ATCGAGATCGCCACCTACAGGAAGCTGCTAGAGGGCGAGGAGAACCGGATCACCATTCCC; GTGCAGACCTTCTCCAACCTGCAGATTCGAGAAACCAGCCTGGACACCAAGTCTGTGTCA; GAAGGCCACCTCAAGAGGAACATCGTGGTGAAGACCGTGGAGATGCGGGATGGAGAGGTC; ATTAAGGAGTCCAAGCAGGAGCACAAGGATGTGATGTGA; ; Clone variation with respect to NM_002055.4; 141 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002055 unedited; GCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTC; ATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGG; CACGAGGGCAGGATGGAGAGGAGACGCATCACCTCCGCTGCTCGCCGCTCCTACGTCTCC; TCAGGGGAGATGATGGTGGGGGGCCTGGCTCCTGGCCGCCGTCTGGGTCCTGGCACCCGC; CTCTCCCTGGCTCGAATGCCCCCTCCACTCCCAACCCGGGTGGATTTCTCCCTGGCTGGG; GCACTCAATGCTGGCTTCAAGGAGACCCGGGCCAGTGAGCGGGCAGAGATGATGGAGCTC; AATGACCGCTTTGCCAGCTACATCGAGAAGGTTCGCTTCCTGGAACAGCAAAACAAGGCG; CTGGCTGCTGAGCTGAACCAGCTGCGGGCCAAGGAGCCCACCAAGCTGGCAGACGTCTAC; CAGGCTGAGCTGCGAGAGCTGCGGCTGCGGCTCGATCAACTCACCGCCAACAGCGCCCGG; CTGGAGGTTGAGAGGGACAATCTGGCACAGGACCTGGCCACTGTGAGGCAGAAGCTCCAG; GATGAAACCAACCTGAGGCTGGAAGCCGAGAACAACCTGGCTGCCTATAGACAGGAAGCA; GATGAAGCCACCCTGGCCCGTCTGGATCTGGAGAGGAAGATTGAGTCGCTGGAGGAGGAG; ATCCGGTTCTTGAGGAAGATCCACGANGAGGGAGGTCGGNAACTNCAGNAGCAGCTGGCC; CGACAGCNAGTCCATGTGGAGCTTGACGTGGCCAGCCAGACCTCACGCAGCCCTGAAAGA; GATCGCACGCAGTATGAGGGCATGGCGTCCAGCACATGCATGAAGCCCGAGAGTGTACCG; CTCCCAGTTGCAGACCTGACGACGCTGCTGNCCGCACGCGNAGCTGNTCCGCCAGGCCAG; CCACGAGCCACGACTACCGNGCCAGNTGCAGTCCTTGACTGCGACCTGNNATCTCTGCNC; GGNACAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002055 unedited; TTGGTCGCTGCCGCAATTTANATCGAGTTTTTTTTTTTTTTTTTTGAGGGCGCAGCATTT; GTCTTTATTTTTCCTCAGCGACTAAAGGCAGCAGCAGTGCCCTGAAGATTAGCAGCAGCA; GCAGCAGCAGGTGGCAGGAAGGAGTGGAGGGAAAGGACACCAAGTCTTGGGGAGAGGGAA; GGACCCTTCTTCGGCCTTAGAGGGGAGAGGAGAACCCTGAAGTGGGCCCTCCCAGTCCCA; TCTCTGGGCACAGATCCCACCAGTCTGCTCACCAGTCTGCTCAGTCAAAGCAGAGTGGGT; GCACTGGGGTGGACGTGTCAGCCCTGAGCACCCGGCCTCCAGGCTGCAGGAATATGAGCC; AGTGTCTTCACTTTGCTCGTGCCTCAGTTTTACAATTGTAAAATAGGGCACTACCTAGAA; TACTGGGTACATTTTGTGTGTGAGTAAGAAGGGACCGCAAGAGGCCCTTGGCTTAGGGAA; AAGCAGCCGGTCACTATGGGGTGGAGAGGGTCAGACGTGTGGCAGTGGCTTACTGGGGTT; AAGAAGCAGCAGTCCCAGGGGCTCAGAGGCTGGGGCGGCCTGGTATGACACAGCAAGGAA; GAGGCCTTTAGAAATGGGACAAAGTCATGCCCTGCCCCCATGGATACATCCCCTTTCTCT; CCTGTTTCAGCATCTTCAAGAGGATGAGTCACTTCCTTAATTCCCACAATCCAGAGGCCA; AGTGCAACTGGTCACCCACAACCCCTACTTGTATGCCTAGCGCCATCCCAATTGCCTCCT; CCTCCATCTCTACCAGCGTCCCCTCCAGTCTGCACGGGGCAGTCCTCCTGGGCTTGACCT; CTCTGTACCCACAGCTGGGGGCCAGGCAGCCCCCCTCTATCCCTCCCAGCACTNATCCCT; CTNCACGTACACCACAGATCCCCCCAGTGCTGAGAAATCAAGCTCCACCTGCCCACAGCG; TGCCCACAGATGGCATC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002055
• Insert Size: 3075 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002055.2, NP_002046.1
• RefSeq Size: 3035 bp
• RefSeq ORF: 1299 bp
• Locus ID: 2670
• UniProt ID: P14136
• Cytogenetics: 17q21.31
• Domains: filament, filament_head
• Protein Families: ES Cell Differentiation/IPS
• Summary: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes a predominant isoform (1, also known as alpha).