GAPDH (NM_002046) Human Untagged Clone

SKU SC118869

GAPDH (untagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GAPDH
• Synonyms: G3PD; GAPD; HEL-S-162eP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002046 edited; ATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCACCAGG; GCTGCTTTTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTGACCTC; AACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCACCGTC; AAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGGAGCGA; GATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGC; GTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGGTCATC; ATCTCTGCCCCCTCTGCTGACGCCCCCATGTTCGTCATGGGTGTGAACCATGAGAAGTAT; GACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAGCACCCCTG; GCCAAGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCC; ATCACTGCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGC; GGGGCTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTC; ATCCCTGAGCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTG; TCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGAAG; GTGGTGAAGCAGGCGTCGGAGGGCCCCCTCAAGGGCATCCTGGGCTACACTGAGCACCAG; GTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTCCACCTTCGACGCTGGGGCTGGC; ATTGCCCTCAACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAACGAATTTGGCTAC; AGCAACAGGGTGGTGGACCTCATGGCCCACATGGCCTCCAAGGAGTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002046 unedited; ACGAGGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTGAN; ACACCATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCA; CCAGGGCTGCTTTTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTG; ACCTCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCA; CCGTCAAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGG; AGCGAGATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCA; CTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGG; TCATCATCTCTGCCCCCTCTGCTGACGCCCCCATGTTCGTCATGGGTGTGAACCATGAGA; AGTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAACAC; CCCTGGCCAAGGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTC; CATGCCATCACTGCCACCCAAAANACTGTGGGATGGCCCCCTNCGGGAAACTGTGGGCGT; GATGGCCCGNGGGGCTCTNCANAACATTATNCCTGCCTNTACTGGGCGCTGCCAAGGCTG; GGGGGCAAGGTCATTCCTTGAACTGAACGGGAANCTCACTGGCATGGGCCTTTCGTGTCC; CCCTGGCAACGTGTTAGGGGGGGGACCTGACCTGCCGTTAAAAAAANCTGCCAAATTGAT; GACCTCAAAAAGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002046 unedited; TTGGTATGGCAACTTCCAGGNCCAGGTANAGCACTGGGGNAGGGTCACAGGNATGCCACC; CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTT; TTTTTTTTTGGTTGAGCACAGGGTACTTTATTGATGGTACATGACAAGGTGCGGCTCCCT; AGGCCCCTCCCCTCTTCAAGGGGTCTACATGGAAACTGTGAGGAGGGGAGATTCAGTGTG; GTGGGGGACTGAGTGTGGCAGGGACTCCCCAGCAGTGAGGGTCTCTCTCTTCCTCTTGTG; CTCTTGCTGGGGCTGGTGGTCCAGGGGTCTTACTCCTTGGAGGCCATGTGGGCCATGAGG; TCCACCACCCTGTTGCTGTAGCCAAATTCGTTGTCATACCAGGAAATGAGCTTGACAAAG; TGGTCGTTGAGGGCAATGCCAGCCCCAGCGTCNAAGGTGGAGGAGTGGGTGTCGCTGTTG; AAGTCAGAGGAGACCACCTGGTGCTCAGTGTANCCCAGGATGCCCTTGAGGGGGCCCTCC; GACGCCTGCTTCACCACCTTCTTGATGTCATCATATTTGGCAGGTTTTTCTAGACGGCAG; GTCAGGTCCACCACTGACACGTTGGCAGTGGGGACACGGAAGGCCATGCCAGTGAGCTTC; CCGTTCAGCTCAGGGATGACCTTGCCCACAGCCTTGGCAGCGCCAGTAAAGGCAGGGATG; ATGTTCTGGAGACCCCGCNGNCATCACGCACAGTTTCCCGGAGGGGGCATCCACAGTCTT; CTGGGTGCANNGATGGCATGGACCTGTGTCATGATTCCTTCACGATACCCAAAGTGCATG; GATGACCTGGNCAGGGTGCTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002046
• Insert Size: 1008 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002046.2, NP_002037.2
• RefSeq Size: 1283 bp
• RefSeq ORF: 1008 bp
• Locus ID: 2597
• UniProt ID: P04406
• Cytogenetics: 12p13.31
• Domains: gpdh
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways
• Summary: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]; Transcript Variant: This variant (1) encodes the longest isoform (1).