MIF (NM_002415) Human Untagged Clone

SKU SC118641

MIF (untagged)-Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MIF
• Synonyms: GIF; GLIF; MMIF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118641 sequence for NM_002415 edited (data generated by NextGen Sequencing); ATGCCGATGTTCATCGTAAACACCAACGTGCCCCGCGCCTCCGTGCCGGACGGGTTCCTC; TCCGAGCTCACCCAGCAGCTGGCGCAGGCCACCGGCAAGCCCCCCCAGTACATCGCGGTG; CACGTGGTCCCGGACCAGCTCATGGCCTTCGGCGGCTCCAGCGAGCCGTGCGCGCTCTGC; AGCCTGCACAGCATCGGCAAGATCGGCGGCGCGCAGAACCGCTCCTACAGCAAGCTGCTG; TGCGGCCTGCTGGCCGAGCGCCTGCGCATCAGCCCGGACAGGGTCTACATCAACTATTAC; GACATGAACGCGGCCAATGTGGGCTGGAACAACTCCACCTTCGCCTAA; ; Clone variation with respect to NM_002415.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002415 unedited; GTCAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGGCGC; GTGCGTCTGTGCCTCTGCGCGGGTCTCCTGGTCCTTCTGCCATCATGCCGATGTTCATCG; TAAACACCAACGTGCCCCGCGCCTCCGTGCCGGACGGGTTCCTCTCCGAGCTCACCCAGC; AGCTGGCGCAGGCCACCGGCAAGCCCCCCCAGTACATCGCGGTGCACGTGGTCCCGGACC; AGCTCATGGCCTTCGGCGGCTCCAGCGAGCCGTGCGCGCTCTGCAGCCTGCACAGCATCG; GCAAGATCGGCGGCGCGCAGAACCGCTCCTACAGCAAGCTGCTGTGCGGCCTGCTGGCCG; AGCGCCTGCGCATCAGCCCGGACAGGGTCTACATCAACTATTACGACATGAACGCGGCCA; ATGTGGGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTCTGCGC; TGGCTCCACCCGGGAACCCGCCGCACGCTGTGTTCTAGGCCCGCCCACCCCAACCTTCTG; GTGGGGAGAAATAAACGGTTTAGAGACTAAAAAAAAAAAAAAAAAACTCGACTCTAGATT; GCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCC; CCAGTGCCTCTCCTGGCCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCCTTGTCCTAA; TAAAATTAAGCTGCATCATTTTGCCTGACCAGGTGTCCCTTCATAATATTATGGGTGGCA; GGGGGTTGCTATGGACCAAGGGGCAAGTTGGGAAGAACACCTGTAGGCCCGCCGGGTCTA; TTGGGACCCAACCGGGAGGCCAGGGACCAATCTGGCTCACTGGAATCCCGCCTCCTGAGT; CAAGCGAATCTCCTGCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002415 unedited; ACTTCCAGGGCCAGGAAGCACTGGGGAGGGTCACAGGGATGCCACCCGGGATCTGTCAGA; AACAGCTATGACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGTCTCT; AAACCGTTTATTTCTCCCCACCAGAAGGTTGGGGTGGGCGGGCCTAGAACACAGCGTGCG; GCGGGTTCCCGGGTGGAGCCAGCGCAGACAGCGTGGGTCCCTGCGGCTCTTAGGCGAAGG; TGGAGTTGTTCCAGCCCACATTGGCCGCGTTCATGTCGTAATAGTTGATGTAGACCCTGT; CCGGGCTGATGCGCAGGCGCTCGGCCAGCAGGCCGCACAGCAGCTTGCTGTAAGAGCGGT; TCTGCGCGCCGCCGATCTTGCCGATGCTGTGCAGGCTGCAGAGCGCGCACGGCTCGNNNG; AGCCGCCGAAGGCCATGAGCTGGTCCGGGACCACGTGCACCGCGATGTACTGGGGGGGCT; TGCCGGTGGCCTGCGCCAGCTGCTGNGTGAGCTCGGAAGGAACCCGTCCGGCACGGAGGC; GCGGGGCACGTTGGTGTTTACGATGAACATCGGCATGATGGCAGAAGGACCAGGAGACCC; GCGCAGAGGCACAGACGCACGCGCCGNCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGA; GTCGTATTCAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTTATAGNACCTCCACCGT; ACACGCCTACCGCCCATNNNNCCAACGGGCCGGGTTATTACGACATTNTGGAAAGTNCCG; TTGATTTTTGTGCCAAACAAACTCTCATTGACGTCAATGGGGTGGGAGACTTGNAAATTC; CCGGAGTTCAACCGCTATTCCGCCCATTGGTGTTCTGCCAAAACGNCACACCATGGNATA; GCGATGACTAATCGTANNAGTACTGCCAGTAGNAAAGTCCCGAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002415
• Insert Size: 560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002415.1, NP_002406.1
• RefSeq Size: 561 bp
• RefSeq ORF: 348 bp
• Locus ID: 4282
• UniProt ID: P14174
• Cytogenetics: 22q11.23
• Domains: MIF
• Protein Families: Druggable Genome
• Protein Pathways: Phenylalanine metabolism, Tyrosine metabolism
• Summary: This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]