PIGF (NM_002643) Human Untagged Clone

SKU SC118483

PIGF (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class F (PIGF), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PIGF
• Synonyms: OORS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118483 sequence for NM_002643 edited (data generated by NextGen Sequencing); ATGAAAGATAACGATATCAAGAGACTACTGTATACCCATCTTTTATGCATATTTTCAATT; ATCCTAAGTGTCTTCATTCCATCACTCTTCTTGGAGAACTTCTCAATATTGGAAACACAC; TTGACATGGTTGTGCATCTGTTCTGGTTTTGTAACTGCTGTCAATCTAGTACTATATTTA; GTAGTGAAACCAAATACATCCTCTAAAAGAAGTTCATTATCACACAAGGTAACTGGATTT; TTGAAATGCTGTATCTACTTTCTTATGTCTTGTTTCTCCTTTCATGTAATTTTTGTTCTG; TATGGAGCACCACTGATAGAGTTGGCATTGGAAACATTTTTATTTGCAGTTATTTTGTCT; ACTTTTACTACTGTGCCTTGCTTATGTTTGTTAGGACCAAACCTCAAAGCATGGCTAAGA; GTGTTCAGTAGAAATGGAGTTACATCCATATGGGAGAATAGTCTCCAGATCACTACAATT; TCTAGCTTTGTAGGAGCATGGCTTGGAGCACTTCCTATTCCACTGGATTGGGAAAGACCA; TGGCAGGTATGGCCCATCTCCTGTACGCTTGGAGCGACCTTTGGCTACGTGGCTGGCCTT; GTTATTTCACCACTCTGGATATACTGGAATAGAAAGCAACTTACATACAAGAACAATTAA; ; Clone variation with respect to NM_002643.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002643 unedited; CTATAGGGCGGCCGCGAATTCGGCACGAGGGTTAGCTAGCCATCCAAGAAAACACCATGA; AAGATAACGATATCAAGAGACTACTGTATACCCATCTTTTATGCATATTTTCAATTATCC; TAAGTGTCTTCATTCCATCACTCTTCTTGGAGAACTTCTCAATATTGGAAACACACTTGA; CATGGTTGTGCATCTGTTCTGGTTTTGTAACTGCTGTCAATCTAGTACTATATTTAGTAG; TGAAACCAAATACATCCTCTAAAAGAAGTTCATTATCACACAAGGTAACTGGATTTTTGA; AATGCTGTATCTACTTTCTTATGTCTTGTTTCTCCTTTCATGTAATTTTTGTTCTGTATG; GAGCACCACTGATAGAGTTGGCATTGGAAACATTTTTATTTGCAGTTATTTTGTCTACTT; TTACTACTGTGCCTTGCTTATGTTTGTTAGGACCAAACCTCAAAGCATGGCTAAGAGTGT; TCAGTAGAAATGGAGTTACATCCATATGGGAGAATAGTCTCCAGATCACTACAATTTCTA; GCTTTGTAGGAGCATGGCTTGGAGCACTTCCTATTCCACTGGATTGNGAAAGACCATGGC; AGGTATGGCCCATCTCCTGTACGCTTGGAGCGACCTTTNNGCTACGTGCTGGCCTTGNNT; ATTACCACTCTGNATATACTGGNANNTAGAAGCACTTACATACCAGAACANTTAACTGGA; GCAAGGGAGATATTTTCTTGTGCAGATTCTGTAGGGNCTGGCANAAATGTGTATGGTCAA; AGCCAGCAGTTCCATTACAGCTCTGTTTTTTACGTAGTACACATGATGTGNATGTAGCTT; TTTAACTATGAAACCCTGAGNAGATGTACCTTCTANTGAANTAANNGTATTATATAGAAA; AAAAANAAAAACTCGCTCTAGATTGNGNCCGCGTCATAGCTGTTCCTGACAGANCCNGNT; GGATCCTGTGACCTNCCCATGCTTCTGNCCTGNAGTGCACTCATGCCACACCTGCTANAA; TA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002643 unedited; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTATTATAAATACTTTATT; TCAACTAGAAGGTACAATCTCTCAGGGGTTTCATAGTTTAAAAAGCTACAATCACATCAT; GTTGTAACTACGTAAAAAACAGAGCTGTAAATGGAACTGCTTGGCTTTGACCATACACAT; TTCTGCCCAGCCCTTACAGAATCTGCACAAAGAAATATCTCCCTTTGCTCCAGTTAATTG; TTCTTGTATGTAAGTTGCTTTCTATTCCAGTATATCCAGAGTGGTGAAATAACAAGGCCA; GCCACGTAGCCAAAGGTCGCTCCAAGCGTACAGGAGATGGGCCATACCTGCCATGGTCTT; TCCCAATCCAGTGGAATAGGAAGTGCTCCAAGCCATGCTCCTACAAAGCTAGAAATTGTA; GTGATCTGGAGACTATTCTCCCATATGGATGTAACTCCATTTCTACTGAACACTCTTAGC; CATGCTTTGAGGTTTGGTCCTAACAAACATAAGCAAGGCACAGTAGTAAAAGTAGACAAA; ATAACTGCAAATAAAAATGTTTCCAATGCCAACTCTATCAGTGGTGCTCCATACAGAACA; AAAATTACATGAAAGGAGAAACAAGACATAAGAAAGTAGATACAGCATTTCAAAAATCCA; GTTACCTTGTGTGATAATGAACTTCCTTTAGAGGATGTATTTGGTTTCACTACTAAATAT; AGTACTAGATTGACAGCAGTTACAATACCAGAACAGATGCACAACCCATGTCAAAGTGTG; TTCCATATTGAGAAAGTCTCCAAGAGAGTGATGGAATGAAGACACTTAGGATAATTGAAA; TATGCATAAAGATGGTATACAGNAGTCTCTGGATATCGTATCTTTCATGAAGTTTTCTAG; GATGCTAGCTACCNTCGTGCCGAATCGCGGCGCCTTAAATGAGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002643
• Insert Size: 970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002643.2, NP_002634.1
• RefSeq Size: 1009 bp
• RefSeq ORF: 660 bp
• Locus ID: 5281
• UniProt ID: Q07326
• Cytogenetics: 2p21
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Summary: This gene encodes a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1).