HTRA1 (NM_002775) Human Untagged Clone

SKU SC118403

HTRA1 (untagged)-Human HtrA serine peptidase 1 (HTRA1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HTRA1
• Synonyms: ARMD7; CADASIL2; CARASIL; HtrA; L56; ORF480; PRSS11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002775 edited; GAATTCGGCACGAGGCTCCGGCCCTCGCCCTGTCCGCCGCCACCGCCGCCGCCGCCAGAG; TCGCCATGCAGATCCCGCGCGCCGCTCTTCTCCCGCTGCTGCTGCTGCTGCTGGCGGCGC; CCGCCTCGGCGCAGCTGTCCCGGGCCGGCCGCTCGGCGCCTTTGGCTGCCGGTTGCCCAG; ACCGCTGCGAGCCGGCGCGCTGCCCGCCGCAGCCGGAGCACTGCGAGGGCGGCCGGGCCC; GGGACGCGTGCGGCTGCTGCGAGGTGTGCGGCGCGCCCGAGGGCGCCGCGTGCGGCCTGC; AGGAGGGCCCGTGCGGCGAGGGGCTGCAGTGCGTGGTGCCCTTCGGGGTGCCAGCCTCGG; CCACGGTGCGGCGGCGCGCGCAGGCCGGCCTCTGTGTGTGCGCCAGCAGCGAGCCGGTGT; GCGGCAGCGACGCCAACACCTACGCCAACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCT; CCGAGAGGCTGCACCGGCCGCCGGTCATCGTCCTGCAGCGCGGAGCCTGCGGCCAAGGGC; AGGAAGATCCCAACAGTTTGCGCCATAAATATAACTTTATCGCGGACGTGGTGGAGAAGA; TCGCCCCTGCCGTGGTTCATATCGAATTGTTTCGCAAGCTTCCGTTTTCTAAACGAGAGG; TGCCGGTGGCTAGTGGGTCTGGGTTTATTGTGTCGGAAGATGGACTGATCGTGACAAATG; CCCACGTGGTGACCAACAAGCACCGGGTCAAAGTTGAGCTGAAGAACGGTGCCACTTACG; AAGCCAAAATCAAGGATGTGGATGAGAAAGCAGACATCGCACTCATCAAAATTGACCACC; AGGGCAAGCTGCCTGTCCTGCTGCTTGGCCGCTCCTCAGAGCTGCGGCCGGGAGAGTTCG; TGGTCGCCATCGGAAGCCCGTTTTCCCTTCAAAACACAGTCACCACCGGGATCGTGAGCA; CCACCCAGCGAGGCGGCAAAGAGCTGGGGCTCCGCAACTCAGACATGGACTACATCCAGA; CCGACGCCATCATCAACTATGGAAACTCGGGAGGCCCGTTAGTAAACCTGGACGGTGAAG; TGATTGGAATTAACACTTTGAAAGTGACAGCTGGAATCTCCTTTGCAATCCCATCTGATA; AGATTAAAAAGTTCCTCACGGAGTCCCATGACCGACAGGCCAAAGGAAAAGCCATCACCA; AGAAGAAGTATATTGGTATCCGAATGATGTCACTCACGTCCAGCAAAGCCAAAGAGCTGA; AGGACCGGCACCGGGACTTCCCAGACGTGATCTCAGGAGCGTATATAATTGAAGTAATTC; CTGATACCCCAGCAGAAGCTGGTGGTCTCAAGGAAAACGACGTCATAATCAGCATCAATG; GACAGTCCGTGGTCTCCGCCAATGATGTCAGCGACGTCATTAAAAGGGAAAGCACCCTGA; ACATGGTGGTCCGCAGGGGTAATGAAGATATCATGATCACAGTGATTCCCGAAGAAATTG; ACCCATAGGCAGAGGCATGAGCTGGACTTCATGTTTCCCTCAAAGACTCTCCCGTGGATG; ACGGATGAGGACTCTGGGCTGCTGGAATAGGACACTCAAGACTTTTGACTGCCATTTTGT; TTGTTCAGTGGAGACTCCCTGGCCAACAGAATCCTTCTTGATAGTTTGCAGGCAAAACAA; ATGTAATGTTGCAGATCCGCAGGCAGAAGCTCTGCCCTTCTGTATCCTATGTATGCAGTG; TGCTTTTTCTTGCCAGCTTGGGCCATTCTTGCTTAGACAGTCAGCATTTGTCTCCTCCTT; TAACTGAGTCATCATCTTAGTCCAACTAATGCAGTCGATACAATGCGTAGATAGAAGAAG; CCCCACGGGAGCCAGGATGGGACTGGTCGTGTTTGTGCTTTTCTCCAAGTCAGCACCCAA; AGGTCAATGCACAGAGACCCCGGGTGGGTGAGCGCTGGCTTCTCAAACGGCCGAAGTTGC; CTCTTTTAGGAATCTCTTTGGAATTGGGAGCACGATGACTCTGAGTTTGAGCTATTAAAG; TACTTCTTACACATTGAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002775 unedited; CAGATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATCGGCACGAGGCTCCGGCCC; TCGCCCTGTCCGCCGCCACCGCCGCCGCCGCCAGAGTCGCCATGCAGATCCCGCGCGCCG; CTCTTCTCCCGCTGCTGCTGCTGCTGCTGGCGGCGCCCGCCTCGGCGCAGCTGTCCCGGG; CCGGCCGCTCGGCGCCTTTGGCTGCCGGTTGCCCAGACCGCTGCGAGCCGGCGCGCTGCC; CGCCGCAGCCGGAGCACTGCGAGGGCGGCCGGGCCCGGGACGCGTGCGGCTGCTGCGAGG; TGTGCGGCGCGCCCGAGGGCGCCGCGTGCGGCCTGCAGGAGGGCCCGTGCGGCGAGGGGC; TGCAGTGCGTGGTGCCCTTCGGGGTGCCAGCCTCGGCCACGGTGCGGCGGCGCGCGCAGG; CCGGCCTCTGTGTGTGCGCCAGCAGCGAGCCGGTGTGCGGCAGCGACGCCAACACCTACG; CCAACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCTCCGAGAGGCTGCACCGGCCGCCGG; TCATCGTCCTGCAGCGCGGAGCCTGCGGCCAAGGGCAGGAAGATCCCAACAGTTTGCGCC; ATAAATATAACTTTATCGCGGACGTGGTGGAGAAGATCGCCCCTGCCGTGGTTCATATCG; AATTGTTTCGCAAGCTTCCGTTTTCTAAACGAGAGGTGCCGGTGGCTAGTGGGTCTGGGT; TTATTGTGTCGGAAGATGGACTGATCGTGACANATGCCCACGTGGTGACCAACAAGCACC; GGGTCAAAAGTGAGCTGAAGAACGGTGCCACTTACGAAGCCAAATCAAGGATGGGGATGA; GAAAGCAGACATCGCACTCATCAAAATTGACCACCCGGGGCAGCCTGCCTGTCCTGCTGC; TTGGCCGCTCCTCAGAGCTGCGCCGGGAAGAATTCGTGTCGCCATCGGAAGACCCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002775 unedited; CTTTGGACCGGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAATGTGTAA; GAAGTACTTTAATAGCTCAAACTCAGAGTCATCGTGCTCCCAATTCCAAAAAGATTCCTA; AAAGAGGCAACTTCGGCCGTTTGAGAAGCCAGCGCTCACCCACCCGGGGTCTCTGTGCAT; TGACCTTTGGGTGCTGACTTGGAGAAAAGCACAAACACGACCAGTCCCATCCTGGCTCCC; GTGGGGCTTCTTCTATCTACGCATTGTATCGACTGCATTAGTTGGACTAAGATGATGACT; CAGTTAAAGGAGGAGACAAATGCTGACTGTCTAAGCAAGAATGGCCCAAGCTGGCAAGAA; AAAGCACACTGCATACATAGGATACAGAAGGGCAGAGCTTCTGCCTGCGGATCTGCAACA; TTACATTTGTTTTGCCTGCAAACTATCAAGAAGGATTCTGTTGGCCAGGGAGTCTCCACT; GAACAAACAAAATGGCAGTCAAAAGTCTTGAGTGTCCTATTCCAGCAGCCCAGAGTCCTC; ATCCGTCATCCACGGAAGAGTCTTTGAGGGAAACATGAAGTCCAGCTCATGCCTCTGCCT; ATGGGTCAATTTCTTCGGGAATCACTGGGATCATGATATCTTTATTACCCCCTGCGACCA; CCATGTTCAGGGGTGCTTTCCCTTTTTAATGACGTCGCTTGACATTCATTGCCGGAAGAC; CACGGGACTGTTCCATTGGATGCCTGATTATGACCGTCGTTTTCCCTTGGAAACCCCCAC; TTTTTGCTTGGGGTATCAGGAATTTACTTCAATAATTTACCGCTTCCTGGAGATCCCGNT; CGGGGAAAGTCCCCGTTGGCCCGGCCCTTTCAGCTTTTTTGCTTTTGCTTGGACCGGAAG; GGACTTCATTTGGGATACCAAAAAACCTTTTTTTTGGGGAAAGCTTTTCCCTTGGCGCCG; TGCGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002775
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002775.3, NP_002766.1
• RefSeq Size: 2133 bp
• RefSeq ORF: 1443 bp
• Locus ID: 5654
• UniProt ID: Q92743
• Cytogenetics: 10q26.13
• Domains: IB, kazal, PDZ, Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Summary: This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]