HTRA1 (NM_002775) Human Untagged Clone

CAT#: SC118403

HTRA1 (untagged)-Human HtrA serine peptidase 1 (HTRA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HTRA1
• Synonyms: ARMD7; CADASIL2; CARASIL; HtrA; L56; ORF480; PRSS11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002775 edited
  GAATTCGGCACGAGGCTCCGGCCCTCGCCCTGTCCGCCGCCACCGCCGCCGCCGCCAGAG
  TCGCCATGCAGATCCCGCGCGCCGCTCTTCTCCCGCTGCTGCTGCTGCTGCTGGCGGCGC
  CCGCCTCGGCGCAGCTGTCCCGGGCCGGCCGCTCGGCGCCTTTGGCTGCCGGTTGCCCAG
  ACCGCTGCGAGCCGGCGCGCTGCCCGCCGCAGCCGGAGCACTGCGAGGGCGGCCGGGCCC
  GGGACGCGTGCGGCTGCTGCGAGGTGTGCGGCGCGCCCGAGGGCGCCGCGTGCGGCCTGC
  AGGAGGGCCCGTGCGGCGAGGGGCTGCAGTGCGTGGTGCCCTTCGGGGTGCCAGCCTCGG
  CCACGGTGCGGCGGCGCGCGCAGGCCGGCCTCTGTGTGTGCGCCAGCAGCGAGCCGGTGT
  GCGGCAGCGACGCCAACACCTACGCCAACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCT
  CCGAGAGGCTGCACCGGCCGCCGGTCATCGTCCTGCAGCGCGGAGCCTGCGGCCAAGGGC
  AGGAAGATCCCAACAGTTTGCGCCATAAATATAACTTTATCGCGGACGTGGTGGAGAAGA
  TCGCCCCTGCCGTGGTTCATATCGAATTGTTTCGCAAGCTTCCGTTTTCTAAACGAGAGG
  TGCCGGTGGCTAGTGGGTCTGGGTTTATTGTGTCGGAAGATGGACTGATCGTGACAAATG
  CCCACGTGGTGACCAACAAGCACCGGGTCAAAGTTGAGCTGAAGAACGGTGCCACTTACG
  AAGCCAAAATCAAGGATGTGGATGAGAAAGCAGACATCGCACTCATCAAAATTGACCACC
  AGGGCAAGCTGCCTGTCCTGCTGCTTGGCCGCTCCTCAGAGCTGCGGCCGGGAGAGTTCG
  TGGTCGCCATCGGAAGCCCGTTTTCCCTTCAAAACACAGTCACCACCGGGATCGTGAGCA
  CCACCCAGCGAGGCGGCAAAGAGCTGGGGCTCCGCAACTCAGACATGGACTACATCCAGA
  CCGACGCCATCATCAACTATGGAAACTCGGGAGGCCCGTTAGTAAACCTGGACGGTGAAG
  TGATTGGAATTAACACTTTGAAAGTGACAGCTGGAATCTCCTTTGCAATCCCATCTGATA
  AGATTAAAAAGTTCCTCACGGAGTCCCATGACCGACAGGCCAAAGGAAAAGCCATCACCA
  AGAAGAAGTATATTGGTATCCGAATGATGTCACTCACGTCCAGCAAAGCCAAAGAGCTGA
  AGGACCGGCACCGGGACTTCCCAGACGTGATCTCAGGAGCGTATATAATTGAAGTAATTC
  CTGATACCCCAGCAGAAGCTGGTGGTCTCAAGGAAAACGACGTCATAATCAGCATCAATG
  GACAGTCCGTGGTCTCCGCCAATGATGTCAGCGACGTCATTAAAAGGGAAAGCACCCTGA
  ACATGGTGGTCCGCAGGGGTAATGAAGATATCATGATCACAGTGATTCCCGAAGAAATTG
  ACCCATAGGCAGAGGCATGAGCTGGACTTCATGTTTCCCTCAAAGACTCTCCCGTGGATG
  ACGGATGAGGACTCTGGGCTGCTGGAATAGGACACTCAAGACTTTTGACTGCCATTTTGT
  TTGTTCAGTGGAGACTCCCTGGCCAACAGAATCCTTCTTGATAGTTTGCAGGCAAAACAA
  ATGTAATGTTGCAGATCCGCAGGCAGAAGCTCTGCCCTTCTGTATCCTATGTATGCAGTG
  TGCTTTTTCTTGCCAGCTTGGGCCATTCTTGCTTAGACAGTCAGCATTTGTCTCCTCCTT
  TAACTGAGTCATCATCTTAGTCCAACTAATGCAGTCGATACAATGCGTAGATAGAAGAAG
  CCCCACGGGAGCCAGGATGGGACTGGTCGTGTTTGTGCTTTTCTCCAAGTCAGCACCCAA
  AGGTCAATGCACAGAGACCCCGGGTGGGTGAGCGCTGGCTTCTCAAACGGCCGAAGTTGC
  CTCTTTTAGGAATCTCTTTGGAATTGGGAGCACGATGACTCTGAGTTTGAGCTATTAAAG
  TACTTCTTACACATTGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002775 unedited
  CAGATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATCGGCACGAGGCTCCGGCCC
  TCGCCCTGTCCGCCGCCACCGCCGCCGCCGCCAGAGTCGCCATGCAGATCCCGCGCGCCG
  CTCTTCTCCCGCTGCTGCTGCTGCTGCTGGCGGCGCCCGCCTCGGCGCAGCTGTCCCGGG
  CCGGCCGCTCGGCGCCTTTGGCTGCCGGTTGCCCAGACCGCTGCGAGCCGGCGCGCTGCC
  CGCCGCAGCCGGAGCACTGCGAGGGCGGCCGGGCCCGGGACGCGTGCGGCTGCTGCGAGG
  TGTGCGGCGCGCCCGAGGGCGCCGCGTGCGGCCTGCAGGAGGGCCCGTGCGGCGAGGGGC
  TGCAGTGCGTGGTGCCCTTCGGGGTGCCAGCCTCGGCCACGGTGCGGCGGCGCGCGCAGG
  CCGGCCTCTGTGTGTGCGCCAGCAGCGAGCCGGTGTGCGGCAGCGACGCCAACACCTACG
  CCAACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCTCCGAGAGGCTGCACCGGCCGCCGG
  TCATCGTCCTGCAGCGCGGAGCCTGCGGCCAAGGGCAGGAAGATCCCAACAGTTTGCGCC
  ATAAATATAACTTTATCGCGGACGTGGTGGAGAAGATCGCCCCTGCCGTGGTTCATATCG
  AATTGTTTCGCAAGCTTCCGTTTTCTAAACGAGAGGTGCCGGTGGCTAGTGGGTCTGGGT
  TTATTGTGTCGGAAGATGGACTGATCGTGACANATGCCCACGTGGTGACCAACAAGCACC
  GGGTCAAAAGTGAGCTGAAGAACGGTGCCACTTACGAAGCCAAATCAAGGATGGGGATGA
  GAAAGCAGACATCGCACTCATCAAAATTGACCACCCGGGGCAGCCTGCCTGTCCTGCTGC
  TTGGCCGCTCCTCAGAGCTGCGCCGGGAAGAATTCGTGTCGCCATCGGAAGACCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002775 unedited
  CTTTGGACCGGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAATGTGTAA
  GAAGTACTTTAATAGCTCAAACTCAGAGTCATCGTGCTCCCAATTCCAAAAAGATTCCTA
  AAAGAGGCAACTTCGGCCGTTTGAGAAGCCAGCGCTCACCCACCCGGGGTCTCTGTGCAT
  TGACCTTTGGGTGCTGACTTGGAGAAAAGCACAAACACGACCAGTCCCATCCTGGCTCCC
  GTGGGGCTTCTTCTATCTACGCATTGTATCGACTGCATTAGTTGGACTAAGATGATGACT
  CAGTTAAAGGAGGAGACAAATGCTGACTGTCTAAGCAAGAATGGCCCAAGCTGGCAAGAA
  AAAGCACACTGCATACATAGGATACAGAAGGGCAGAGCTTCTGCCTGCGGATCTGCAACA
  TTACATTTGTTTTGCCTGCAAACTATCAAGAAGGATTCTGTTGGCCAGGGAGTCTCCACT
  GAACAAACAAAATGGCAGTCAAAAGTCTTGAGTGTCCTATTCCAGCAGCCCAGAGTCCTC
  ATCCGTCATCCACGGAAGAGTCTTTGAGGGAAACATGAAGTCCAGCTCATGCCTCTGCCT
  ATGGGTCAATTTCTTCGGGAATCACTGGGATCATGATATCTTTATTACCCCCTGCGACCA
  CCATGTTCAGGGGTGCTTTCCCTTTTTAATGACGTCGCTTGACATTCATTGCCGGAAGAC
  CACGGGACTGTTCCATTGGATGCCTGATTATGACCGTCGTTTTCCCTTGGAAACCCCCAC
  TTTTTGCTTGGGGTATCAGGAATTTACTTCAATAATTTACCGCTTCCTGGAGATCCCGNT
  CGGGGAAAGTCCCCGTTGGCCCGGCCCTTTCAGCTTTTTTGCTTTTGCTTGGACCGGAAG
  GGACTTCATTTGGGATACCAAAAAACCTTTTTTTTGGGGAAAGCTTTTCCCTTGGCGCCG
  TGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002775
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002775.3, NP_002766.1
• RefSeq Size: 2133 bp
• RefSeq ORF: 1443 bp
• Locus ID: 5654
• UniProt ID: Q92743
• Cytogenetics: 10q26.13
• Domains: IB, Tryp_SPc, PDZ, kazal
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]