Suppressor of Ty 4 homolog 1 (SUPT4H1) (NM_003168) Human Untagged Clone

SKU SC118150

SUPT4H1 (untagged)-Human suppressor of Ty 4 homolog 1 (S. cerevisiae) (SUPT4H1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Suppressor of Ty 4 homolog 1
• Synonyms: SPT4; SPT4H; Supt4a; SUPT4H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003168, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCCTGGAGACGGTGCCGAAGGACCTGCGGCATCTGCGGGCCTGTTTGCTGTGTTCGCTGGTCAAGA; CTATAGACCAGTTTGAATATGATGGTTGTGACAATTGTGATGCATATCTACAAATGAAGGGTAACCGAGA; GATGGTATATGACTGCACTAGCTCTTCCTTTGATGGAATCATTGCGATGATGAGTCCAGAGGACAGCTGG; GTCTCCAAGTGGCAGCGAGTCAGTAACTTTAAGCCAGGTGTATATGCGGTGTCAGTCACTGGTCGCCTGC; CCCAAGGAATCGTGCGGGAGCTGAAAAGTCGAGGAGTGGCCTACAAATCCAGAGACACAGCTATAAAGAC; CTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003168 unedited; CCGTCCNGATTTGTATACGATTCCTATAGGGCGGCCGCGATTCGGCACGAGGGTCGTCTA; TCTCCCTGTTGTTCTTCCCTCGGCGAAGAGGCCCTGGAGACGGTGCCGAAGGACCTGCGG; CATCTGCGGGCCTGTTTGCTGTGTTCCCTGGTCAAGACTATAGACCAGTTTGAATATGAT; GGTTGTGACAATTGTGATGCATATCTACAAATGAAGGGTAACCGAGAGATGGTATATGAC; TGCACTAGCTCTTCCTTTGATGGAATCATTGCGATGATGAGTCCAGAGGACAGCTGGGTC; TCCAAGTGGCAGCGAGTCAGTAACTTTAAGCCAGGTGTATATGCGGTGTCAGTCACTGGT; CGCCTGCCCCAAGGAATCGTGCGGGAGCTGAAAAGTCGAGGAGTGGCCTACAAATCCAGA; GACACAGCTATAAAGACCTAGCAAGATGCAAGGCTGCCAGCATCTTTGCTCTCCACCTCC; TGCCTCTGCTTATTTCTTGTTCTGGAACTAAATGAACAGAACTTCAAATACTTCCTACCC; TCCAATTCAGACTCAGCTGACTGTTGAGAGAGCAGCACATCATTTTATCATTTTATCTTC; TTTGGACTACAGGTGGGGTGGGAGGGATTTGGGTTGGTGGATTAACAGATGGAATTGAGG; AGAGAGTAGGATGCTGATTTTCCTACCCGTGGCCCAGGTCTGTGCCTTCCCCATGCCAAG; GACTCTAGGTCAAATGTCAATAAATATGAACCTCGAGAAAGTTCTGAAGGCCATGAAAAA; AAAAAAAAAAAAAAAAACTCGACTCTAGAATTGCGGCCGCGTCATAGCTGTTTCCTGAAC; AGATCCCCGGTGGCATCCCTGTGACCCCTNCCCAGTGCCTCTCTGCCCCTGGAAGTTGCC; ACCCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003168 unedited; GCTATGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTACAGGCC; TTCAAAACTTTCTCGAGGTTCATATTTATTGACATTTGACCTAAAGTCCTTGGCATGGGG; AAGGCACAGACCTGGGCCACGGGTAGGAAAATCAGCATCCTACTCTCTCCTCAATTCCAT; CTGTTAATCCACCAACCCAAATCCCTCCCACCCCACCTGTAGTCCAAAGAAGATAAAATG; ATAAAATGATGTGCTGCTCTCTCAACAGTCAGCTGAGTCTGAATTGGAGGGTAGGAAGTA; TTTGAAGTTCTGTTCATTTAGTTCCAGAACAAGAAATAAGCAGAGGCAGGAGGTGGAGAG; CAAAGATGCTGGCAGCCTTGCATCTTGCTAGGTCTTTATAGCTGTGTCTCTGGATTTGTA; GGCCACTCCTCGACTTTTCAGCTCCCGCACGATTCCTTGGGGCAGGCGACCAGTGACTGA; CACCGCATATACACCTGGCTTAAAGTTACTGACTCGCTGCCACTTGGAGACCCAGCTGTC; CTCTGGACTCATCATCGCAATGATTCCATCAAAGGAAGAGCTAGTGCAGTCATATACCAT; CTCTCGGTTACCCTTCATTTGTAGATATGCATCACAATTGTCACAACCATCATATTCAAA; CTGGTCTATAGTCTTGACCAGCGAACACAGNANACAGGCCCGCAGATGCCGCAGGTCCTT; CGGCACCGTCTCCAGGGCCCATCTTCGCCGATGGAAAGAACACCAGGGAGATAGACGACC; CTCGTGCCGAATCCCTGCCGCCCTATAGTGAGTCGGATTACAAAATCCTGACGGTTACTT; AACGAGCTCTGCTTAAATAGACTCCCCACGGACCACGCTACCGCCCATTGGTGCAACGGG; GCGGGTTATTACCACCTTTGGAAGCCCGGTGGTTTTGTGCCAACAACTCCATGGCGCAAT; GGGGGAAACTGGAATCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003168
• Insert Size: 780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003168.1, NP_003159.1
• RefSeq Size: 1499 bp
• RefSeq ORF: 354 bp
• Locus ID: 6827
• UniProt ID: P63272
• Cytogenetics: 17q22
• Protein Families: Transcription Factors
• Summary: This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]; Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein.