SPOP (NM_003563) Human Untagged Clone

SKU SC117899

SPOP (untagged)-Human speckle-type POZ protein (SPOP), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SPOP
• Synonyms: BTBD32; NEDMACE; NEDMIDF; NSDVS1; NSDVS2; TEF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003563 edited; GAATTCGGCACGAGGCGCGCTCCGAGTGTGTGTGTATTTGTGTATCGGCGGTCCCGCAGG; TCCCGGATGTTGCGGACAGTATGAGGCAAGCGCAGGGGGACGGGGACCAGCAGCTGTCGC; CGCCGCTCTCAGATCGAGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCGATCTC; GGCTCACTGCCACCTTTGCCTCCTGGGTTCAAGCGATTCTTCTGCCTCAGCCTCCCGAGT; AGCTGGGATTACAGGGTGAAGAGGGAACAGAAATCTTTGCCCCCTGACTTTGGAAATCTC; GTTTAACCTTCAAACTGGCGATGTCAAGGGTTCCAAGTCCTCCACCTCCGGCAGAAATGT; CGAGTGGCCCCGTAGCTGAGAGTTGGTGCTACACACAGATCAAGGTAGTGAAATTCTCCT; ACATGTGGACCATCAATAACTTTAGCTTTTGCCGGGAGGAAATGGGTGAAGTCATTAAAA; GTTCTACATTTTCATCAGGAGCAAATGATAAACTGAAATGGTGTTTGCGAGTAAACCCCA; AAGGGTTAGATGAAGAAAGCAAAGATTACCTGTCACTTTACCTGTTACTGGTCAGCTGTC; CAAAGAGTGAAGTTCGGGCAAAATTCAAATTCTCCATCCTGAATGCCAAGGGAGAAGAAA; CCAAAGCTATGGAGAGTCAACGGGCATATAGGTTTGTGCAAGGCAAAGACTGGGGATTCA; AGAAATTCATCCGTAGAGATTTTCTTTTGGATGAGGCCAACGGGCTTCTCCCTGATGACA; AGCTTACCCTCTTCTGCGAGGTGAGTGTTGTGCAAGATTCTGTCAACATTTCTGGCCAGA; ATACCATGAACATGGTAAAGGTTCCTGAGTGCCGGCTGGCAGATGAGTTAGGAGGACTGT; GGGAGAATTCCCGGTTCACAGACTGCTGCTTGTGTGTTGCCGGCCAGGAATTCCAGGCTC; ACAAGGCTATCTTAGCAGCTCGTTCTCCGGTTTTTAGTGCCATGTTTGAACATGAAATGG; AGGAGAGCAAAAAGAATCGAGTTGAAATCAATGATGTGGAGCCTGAAGTTTTTAAGGAAA; TGATGTGCTTCATTTACACGGGGAAGGCTCCAAACCTCGACAAAATGGCTGATGATTTGC; TGGCAGCTGCTGACAAGTATGCCCTGGAGCGCTTAAAGGTCATGTGTGAGGATGCCCTCT; GCAGTAACCTGTCCGTGGAGAACGCTGCAGAAATTCTCATCCTGGCCGACCTCCACAGTG; CAGATCAGTTGAAAACTCAGGCAGTGGATTTCATCAACTATCATGCTTCGGATGTCTTGG; AGACCTCTGGGTGGAAGTCAATGGTGGTGTCACATCCCCACTTGGTGGCTGAGGCATACC; GCTCTCTGGCTTCAGCACAGTGCCCTTTTCTGGGACCCCCACGCAAACGCCTGAAGCAAT; CCTAAGATCCTGCTTGTTGTAAGACTCCGTTTAATTTCCAGAAGCAGCAGCCACTGTTGC; TGCCACTGACCACCAGGTAGACAGCGCAATCTGTGGAGCTTTTACTCTGTTGTGAGGGGA; AGAGACTGCATTGTGGCCCCAGACTTTTAAAACAGCACTAAATAACTTGGGGGAAACGGG; GGGAGGGAAAATGAAATGAAAACCCTGTTGCTGCGTCACTGTGTTCCCTTTGGCCTGGCT; GAGTTTGATACTGTGGGGATTCAGTTTAGGCGCTGGCCCGAGGATATCCCAGCGGTGGTA; CTTCGGAGACACCTGTCTGCATCTGACTGAGCAGAACAAATCGTCAGGTGCCTGGAGCAA; AAAGGAAAAAAAAAAAAGAAAGGACATTGAGTTTTAACAGAAGGGAAAAGGAAAGAAGAA; AAGATTTTTGCAGAATTTCTCAAAAATCAGTTTGTGGATTCCAGTAGTATTTATATTGAG; AGAAACAAATTTTAGTCCTTCTAACTGTGCTAAAACTTGGATATTTGTGAAAACTCCTTA; CCACCATACAAGCATCAGAAGAGCTCTCTTGTTGTTAGCACTTATTGTTTGCAAGAACAG; AATACATCCTTTTATCCTTTTATGAAAAATGACAAGTGAAGGCAAAAGGGGAAGGTTATT; TGATCTGGAAGATGAGTGTTCTGATGTGGTGGCTTTTGCAAAAATCTTTATTGGTGTTGA; AAACTGGAAAAAATAACTCATCCAGAATTCATATTGTCTTGACAAGAACTATGGTTCTCT; GTTTTTAGATATTGTGGAAAATGTTTTTGGGCATTTTTCTCTGATTTTATTTCTTCTCCC; CCACCCCTTTTTCTAAAAAACAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003563 unedited; NGGGTCGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGCGCTCC; GAGTGTGTGTGTATTTGTGTATCGGCGGTCCCGCAGGTCCCGGATGTTGCGGACAGTATG; AGGCAAGCGCAGGGGGACGGGGACCAGCAGCTGTCGCCGCCGCTCTCAGATCGAGTCTTG; CTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCCACCTTTGCCTCC; TGGGTTCAAGCGATTCTTCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGGTGAAGAG; GGAACAGAAATCTTTGCCCCCTGACTTTGGAAATCTCGTTTAACCTTCAAACTGGCGATG; TCAAGGGTTCCAAGTCCTCCACCTCCGGCAGAAATGTCGAGTGGCCCCGTAGCTGAGAGT; TGGTGCTACACACAGATCAAGGTAGTGAAATTCTCCTACATGTGGACCATCAATAACTTT; AGCTTTTGCCGGGAGGAAATGGGTGAAGTCATTAAAAGTTCTACATTTTCATCAGGAGCA; AATGATAAACTGAAATGGTGTTTGCGAGTAAACCCCAAAGGGTTAGATGAAGAAAGCAAA; GATTACCTGTCACTTTACCTGTTACTGGTCAGCTGTCCAAAGAGTGAAGTTCGGGCAAAA; TTCAAATTCTCCATCCTGAATGCCAAGGGAGAAGAAACCAAAGCTATGGAGAGTCAACGG; GCATATAGGGTTTGTGCAAGGCAAAGACTGGGGATTCAAGAAATTCATCCGTAGAGATTN; TCTTTTTGGATGAGGCCAACGGGCTTCTCCCTGATGACAAGCTTACCCTCTCTGCGAGGG; NGAAGTGTTGTGCAAGATTCTGTCACCATTTCTGGGCAGAATACATGACTGNGNGNAAGG; TTCCCTGAGTGCCGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003563 unedited; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTTAAAAAAGGGGTG; GGGGAGAAGAAATAAAATCGAGAAAATGCCCAAAAACATTTTCCCAATATCTAAAAACAG; AGAACCATAGTTCTTGTCAAGACAATATGAATTCTGGATGAGTTATTTTTTCCAGTTTTC; AACACCAATAAAGATTTTTGCAAAAGCCACCACATCAGAACACTCATCTTCCAGATCAAA; TAACCTTCCCCTTTTGCCTTCACTTGTCATTTTTCATAAAAGGATAAAAGGATGTATTCT; GTTCTTGCAAACAATAAGTGCTAACAACAAGAGAGCTCTTCTGATGCTTGTATGGTGGTA; AGGAGTTTTCACAAATATCCAAGTTTTAGCACAGTTAGAAGGACTAAAATTTGTTTCTCT; CAATATAAATACTACTGGAATCCACAAACTGATTTTTGAGAAATTCTGCAAAAATCTTTT; CTTCTTTCCTTTTCCCTTCTGTTAAAACTCAATGTCCTTTCTTTTTTTTTTTTCCTTTTT; GCTCCAGGCACCTGACGATTTGTTCTGCTCAGTCAGATGCAGACAGGTGTCTCCGAAGTA; CCACCGCTGGGATATCCTCGGGCCAGCGCCTAAACTGAATCCCCACAGTATCAAACTCAG; CCAGGCCAAAGGGAACACAGTGACGCAGCAACAGGGTTTTCATTTCATTTTCCCTCCCCC; GTTTCCCCCCAGTTATTTAGTGCTGTTTTAAAAGTCTGGGGCCACAATGCAGTCTCTTCC; CCTCACACAGAGTAAAAGCTCCACAGATGCGCTGTCTACCTGGTGGTCAGTGGCAGCACA; GTGGCTGCTGCTTCTGGAANTAAACGGAGTCTTACANNCAGCAGATCTTANGGATGCTCA; GGCGTTGCGTGGGGGGTCCANAAAGGCACTGTGCTGAACCANNAAGCGGATGCCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003563
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003563.3, NP_003554.1
• RefSeq Size: 3053 bp
• RefSeq ORF: 1125 bp
• Locus ID: 8405
• UniProt ID: O43791
• Cytogenetics: 17q21.33
• Domains: BTB, MATH
• Summary: This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Transcript variants 1-6 encode the same protein.