DEGS1 (NM_003676) Human Untagged Clone

SKU SC117856

DEGS1 (untagged)-Human degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) (DEGS1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DEGS1
• Synonyms: DEGS; DEGS-1; Des-1; DES1; FADS7; HLD18; MIG15; MLD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117856 sequence for NM_003676 edited (data generated by NextGen Sequencing); ATGGGGAGCCGCGTCTCGCGGGAAGACTTCGAGTGGGTCTACACCGACCAGCCGCACGCC; GACCGGCGCCGGGAGATCCTGGCAAAGTATCCAGAGATAAAGTCCTTGATGAAACCTGAT; CCCAATTTGATATGGATTATAATTATGATGGTTCTCACCCAGTTGGGTGCATTTTACATA; GTAAAAGACTTGGACTGGAAATGGGTCATATTTGGGGCCTATGCGTTTGGCAGTTGCATT; AACCACTCAATGACTCTGGCTATTCATGAGATTGCCCACAATGCTGCCTTTGGCAACTGC; AAAGCAATGTGGAATCGCTGGTTTGGAATGTTTGCTAATCTTCCTATTGGGATTCCATAT; TCAATTTCCTTTAAGAGGTATCACATGGATCATCATCGGTACCTTGGAGCTGATGGCGTC; GATGTAGATATTCCTACCGATTTTGAGGGCTGGTTCTTCTGTACCGCTTTCAGAAAGTTT; ATATGGGTTATTCTTCAGCCTCTCTTTTATGCCTTTCGACCTCTGTTCATCAACCCCAAA; CCAATTACGTATCTGGAAGTTATCAATACCGTGGCACAGGTCACTTTTGACATTTTAATT; TATTACTTTTTGGGAATTAAATCCTTAGTCTACATGTTGGCAGCATCTTTACTTGGCCTG; GGTTTGCACCCAATTTCTGGACATTTTATAGCTGAGCATTACATGTTCTTAAAGGGTCAT; GAAACTTACTCATATTATGGGCCTCTGAATTTACTTACCTTCAATGTGGGTTATCATAAT; GAACATCATGATTTCCCCAACATTCCTGGAAAAAGTCTTCCACTGGTGAGGAAAATAGCA; GCTGAATACTATGACAACCTCCCTCACTACAATTCCTGGATAAAAGTACTGTATGATTTT; GTGATGGATGATACAATAAGTCCCTACTCAAGAATGAAGAGGCACCAAAAAGGAGAGATG; GTGCTGGAGTAA; ; Clone variation with respect to NM_003676.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003676 unedited; NGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAGCCGC; CGCCGCCGCCGCCACCTCTGAGCAGCCGGCTGGGAGCGAGAGCCGACAGCTAGTCTGCAA; GCCACCGCTGTCGCCATGGGGAGCCGCGTCTCGCGGGAAGACTTCGAGTGGGTCTACACC; GACCAGCCGCACGCCGACCGGCGCCGGGAGATCCTGGCAAAGTATCCAGAGATAAAGTCC; TTGATGAAACCTGATCCCAATTTGATATGGATTATAATTATGATGGTTCTCACCCAGTTG; GGTGCATTTTACATAGTAAAAGACTTGGACTGGAAATGGGTCATATTTGGGGCCTATGCG; TTTGGCAGTTGCATTAACCACTCAATGACTCTGGCTATTCATGAGATTGCCCACAATGCT; GCCTTTGGCAACTGCAAAGCAATGTGGAATCGCTGGTTTGGAATGTTTGCTAATCTTCCT; ATTGGGATTCCATATTCAATTTCCTTTAAGAGGTATCACATGGATCATCATCGGTACCTT; GGAGCTGATGGCGTCGATGTAGATATTCCTACCGATTTTGAGGGCTGGTTCTTCTGTACC; GCTTTCAGAAAGTTTATATGGGTTATTCTTCAGCCTCTCTTTTATGCCTTTCGACCTCTG; TTCATCAACCCCAAACCAATTACGTATCTGGAAGTTATCAATACCGTGGCACAGGTCACT; TTTGACATTNTAATTTATTACTTTNTGGGAATTAAATCCTTAGTCTACATGNTGGCAGCA; TCTTTACTTGGCCTGGGTTTGCACCCCATTTCTGGACATTNTATAGCTGAGCATTTACAT; GTCTTAAAGGGTCATGAAACTTACTCATATTATGGGCCTCTGAATTTACTTACCTTCATG; TGGGTTATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003676 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAATTTGAATGGTGGAAATTCA; GTAAGTACTTTATTTATAAGTAAGGCTGTACAGGATTCAGCAGTGTCTAATTCGGGTCCC; ATATAGAAGTTAAAATTTTTAAATGAGTAATTCAGCTTACAGGGAAATATATTTTCATCT; CAATCTTTTATGCTATATCATACCTCAGGCTTTTTAAAAATTATTTTTGAGATGAAATTT; TAAAGTTCCCCCGGTACCCAAGTTACACTATTATATAGTATAAAGATAGTGAAATACAAA; AATGTAACAAATACTAATACACTGTGATTAATAGCTGTTTATTAGCTTTTTAATAAAGCA; AAACTTTATAGTGAAATAAACTAGAAACAGCTTTTTATTTTTTATATATATTTATTTATT; TTCGAGATGGGGTCTTGCTCTGTCGCCCAGGGTGGAGTGCAGCGGCACAATTCTTGCGTC; ACTGCAGCCTCTGCCTNCGCCTCCTGGGTTCAAGCAATTCTTCCCCCTTAGCCCTCCCAT; GTAGCCTGGGATTACAGGGCATGTGCCCGCCACGTCCCGGCTAATTCTTGCATCCCTAGT; ACAGCATGGGGCTTCACCCGTGTTGGCCCAGGCTGGCCTTCGACCCCTTGACCTTAGGTG; ATCCACCCCACCTGGCCCTCCCAAGGGCTGGGACTATAGGCATGACCCCCCGTCCCCGGC; GACAAAACTACTCAAAGCCTCTTCAAGGACCGGCTATAAAATGCCGACCTGACCGAAACC; TTCCCATGGACCACCCCCCCCAACACAAGTTTCTTGCGAGGAAACCGGGCCACACGAACC; CGGGTGACTGTTTAAAGCCACATTCACTCCTTGATCAACCGGACCTCTACCTGGAAATGG; GCCCGGGGAGCTTTGGCCTCCCTGGTTCAAGTTAAAATGCAAACCCCCTTTACCCACTAA; TCCGGGAAACACCCCTGGGCCCATGTATTTACTCCACCCCTCTTCTTTTGGGCCCTTCTT; CTCGAGGGGCCTCTGGTTACCCTACAAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_003676
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003676.2, NP_003667.1
• RefSeq Size: 2058 bp
• RefSeq ORF: 972 bp
• Locus ID: 8560
• UniProt ID: O15121
• Cytogenetics: 1q42.11
• Domains: FA_desaturase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Summary: This gene encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. [provided by RefSeq, Mar 2010]