Beclin 1 (BECN1) (NM_003766) Human Untagged Clone

CAT#: SC117750

BECN1 (untagged)-Human beclin 1, autophagy related (BECN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Beclin 1
• Synonyms: ATG6; beclin1; VPS30
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117750 sequence for NM_003766 edited (data generated by NextGen Sequencing)
  ATGGAAGGGTCTAAGACGTCCAACAACAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGC
  TGCAGCCAGCCCCTGAAACTGGACACGAGTTTCAAGATCCTGGACCGTGTCACCATCCAG
  GAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGGAGAGACCCAGGAGGAA
  GAGACTAACTCAGGAGAGGAGCCATTTATTGAAACTCCTCGCCAGGATGGTGTCTCTCGC
  AGATTCATCCCCCCAGCCAGGATGATGTCCACAGAAAGTGCCAACAGCTTCACTCTGATT
  GGGGAGGCATCTGATGGCGGCACCATGGAGAACCTCAGCCGAAGACTGAAGGTCACTGGG
  GACCTTTTTGACATCATGTCGGGCCAGACAGATGTGGATCACCCACTCTGTGAGGAATGC
  ACAGATACTCTTTTAGACCAGCTGGACACTCAGCTCAACGTCACTGAAAATGAGTGTCAG
  AACTACAAACGCTGTTTGGAGATCTTAGAGCAAATGAATGAGGATGACAGTGAACAGTTA
  CAGATGGAGCTAAAGGAGCTGGCACTAGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGAC
  GTGGAAAAGAACCGCAAGATAGTGGCAGAAAATCTCGAGAAGGTCCAGGCTGAGGCTGAG
  AGACTGGATCAGGAGGAAGCTCAGTATCAGAGAGAATACAGTGAATTTAAACGACAGCAG
  CTGGAGCTGGATGATGAGCTGAAGAGTGTTGAAAACCAGATGCGTTATGCCCAGACGCAG
  CTGGATAAGCTGAAGAAAACCAACGTCTTTAATGCAACCTTCCACATCTGGCACAGTGGA
  CAGTTTGGCACAATCAATAACTTCAGGCTGGGTCGCCTGCCCAGTGTTCCCGTGGAATGG
  AATGAGATTAATGCTGCTTGGGGCCAGACTGTGTTGCTGCTCCATGCTCTGGCCAATAAG
  ATGGGTCTGAAATTTCAGAGATACCGACTTGTTCCTTACGGAAACCATTCATATCTGGAG
  TCTCTGACAGACAAATCTAAGGAGCTGCCGTTATACTGTTCTGGGGGGTTGCGGTTTTTC
  TGGGACAACAAGTTTGACCATGCAATGGTGGCTTTCCTGGACTGTGTGCAGCAGTTCAAA
  GAAGAGGTTGAGAAAGGCGAGACACGTTTTTGTCTTCCCTACAGGATGGATGTGGAGAAA
  GGCAAGATTGAAGACACAGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAAC
  TCTGAGGAGCAGTGGACAAAAGCTCTCAAGTTCATGCTGACGAATCTTAAGTGGGGTCTT
  GCTTGGGTGTCCTCACAATTTTATAACAAATGA
  
  Clone variation with respect to NM_003766.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003766 unedited
  AAACAAACTTTACTATAGGCGGCCGCGCAATTCGCACGAGGCGGGAAGTCGTTGAAGACA
  GAGCGATGGTAGTTCTGGAGGCCTCGCTCCGGGGCCGACCCGAGGCCACAGTGCCTCCGC
  GGTAGACCGGACTTGGGTGACGGGCTCCGGGCTCCCGAGGTGAAGAGCATCGGGGGCTGA
  GGGATGGAAGGGTCTAAGACGTCCAACAACAGCACCATGCAGGTGAGCTTCGTGTGCCAG
  CGCTGCAGCCAGCCCCTGAAACTGGACACGAGTTTCAAGATCCTGGACCGTGTCACCATC
  CAGGAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGGAGAGACCCAGGAG
  GAAGAGACTAACTCAGGAGAGGAGCCATTTATTGAAACTCCTCGCCAGGATGGTGTCTCT
  CGCAGATTCATCCCCCCAGCCAGGATGATGTCCACAGAAAGTGCCAACAGCTTCACTCTG
  ATTGGGGAGGCATCTGATGGCGGCACCATGGAGAACCTCAGCCGAAGACTGAAGGTCACT
  GGGGACCTTTTTGACATCATGTCGGGCCAGACAGATGTGGATCACCCACTCTGTGAGGAA
  TGCACAGATACTCTTTTAGACCAGCTGGACACTCAGCTCAACGTCACTGAAAATGAGTGT
  CAGAACTACAAACGCTGTTTGGAGATCTTAGAGCAAATGAATGAGGATGACAGTGAACAG
  TTACAGATGGAGCTAAAGGAGCTGGCACTAGAGGAGGAGAGGCTGATCCAGGAGCTGGAA
  GACGTGGAAAAAGAACCGCAGATAGTGGCAGAAAATCTCGAGAAGGTCCANNGCTGAGCT
  GANAGACTGGATCANGNAGAAGCTCANTATCAGAGAGAATACAGTGAATTTAAACGACAG
  CAGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003766 unedited
  CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAGTTTTCAG
  ACTGCAGCAAATCTTTTATTACAAATAATTAAATCTCTCCATAATGTCTCAAACAGTATC
  AAACACCATTTCATATCTCTAACACAGAGCAGAGTCGGCATTCAGTATAAGAACCAAGTG
  AAAAGTGTTAAATTTCAAGCATCTGATCACATCACATGGTGACCAGGTAAAGCTTAGATG
  TCATTTTCCCACATTATCCAACTGTGCATCTCAAACATATCCTCATCTCAGTAAAGACAA
  AAGTTTCTATTTCATATTGTTAAGTGCAGGAAGTTGAGAGAGATAAAAATCCAGTGAAAA
  CACATCAATCTCAATTCAACTCAGTTAAAAAAAAGAAAAGCAAATTTAAATTAGTTTTTT
  TCAGAGAAGAAAGGGAAAGGAGTCCATGGGGTTAAGAATCAAAACTGACCAGGGCTGGCA
  ACTATAGATGGCATGTTGTAGCTCTGGAAAGTATCTGTCACATGATATTTTAAAATAAAG
  TGGCTTTTGTGGATTTTTTCTTTTTTGGTATTGTAAACATGTACTGTTTAATATTACCCG
  AATTTAATTTAAAACATGTTTGCAAACAAAACAAAATTAAAAGCCTTTAAGGCAAACCCT
  CCCCCTAAGGAAAAAAGTCATTTGTTATAAAATTGTGAGGACACCNCAAGCAGACCCCAC
  TTAAGATTCGTCAGCATGAACTTGAGAGCTTTTGTCCACTGCTCCTCAGATTAAACTGGG
  TTTTGATGGAATAGACCCGCACTGCCTNCTGTGTCTCAATCTGGCTTTCTNCCATCCATC
  TGTAGGGAGACANAACGTGTCTCGCCTTCTCAACCTCTCTTTGACTGCTGCCCACAGTCA
  GAAAGCACATGCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003766
• Insert Size: 2240 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003766.2, NP_003757.1
• RefSeq Size: 2144 bp
• RefSeq ORF: 1353 bp
• Locus ID: 8678
• UniProt ID: Q14457
• Cytogenetics: 17q21.31
• Domains: APG6
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy
• Gene Summary: This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 encode the same isoform.