TIMELESS (NM_003920) Human Untagged Clone

CAT#: SC117684

TIMELESS (untagged)-Human timeless homolog (Drosophila) (TIMELESS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIMELESS
• Synonyms: hTIM; TIM; TIM1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003920, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACTTGCACATGATGAACTGTGAACTTCTAGCCACATGTAGTGCCCTTGGGTACTTGGAGGGAGACA
  CTTACCATAAGGAACCAGATTGCTTAGAGAGCGTGAAGGATCTGATCCGCTATTTGAGGCATGAGGATGA
  GACACGAGATGTGCGGCAGCAGCTGGGGGCAGCCCAGATCCTACAGAGCGACCTTCTGCCCATCCTCACC
  CAGCACCACCAGGACAAGCCTCTCTTTGATGCTGTTATCAGACTGATGGTGAACTTGACACAACCAGCCT
  TGCTCTGTTTTGGCAATCTGCCTAAGGAGCCCAGCTTTCGGCACCATTTTTTGCAGGTGCTAACTTATTT
  GCAGGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCTTTTGGAGTCCTCAGTGAAACCTTGTATGAGCTG
  CTGCAGCTGGGCTGGGAGGAACGGCAGGAGGAAGACAACTTGCTGATTGAACGGATCCTACTGCTGGTCA
  GAAATATTCTCCATGTCCCAGCTGACCTTGATCAGGAGAAGAAGATTGATGATGACGCCAGTGCCCATGA
  CCAGCTCCTCTGGGCGATTCACCTCAGCGGCCTGGATGACCTGCTCCTCTTTCTGGCCAGCTCGTCTGCT
  GAGGAGCAATGGAGCCTACATGTGCTAGAGATTGTCTCCCTTATGTTTCGTGACCAGAACCCCGAGCAGC
  TGGCGGGAGTAGGGCAGGGACGCTTAGCTCAGGAGCGGAGTGCAGATTTTGCAGAACTGGAGGTGTTGCG
  CCAGCGAGAGATGGCAGAAAAGAAGACTCGAGCCCTCCAGCGAGGCAACAGGCATTCTCGATTTGGGGGC
  TCCTATATTGTCCAGGGGTTGAAATCCATTGGGGAGAGGGACCTCATCTTTCACAAAGGCCTTCACAACC
  TACGAAACTACAGTTCAGATTTGGGAAAGCAGCCGAAAAAGGTGCCTAAACGTCGCCAGGCCGCCCGAGA
  GCTGTCCATTCAGCGCCGTTCTGCCCTCAATGTGAGGCTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG
  GAGAACTGTTACAACCGGCTCATGGGATCAGTAAAGGATCACCTGCTTCGGGAGAAAGCTCAGCAGCATG
  ATGAGACCTATTATATGTGGGCCTTGGCTTTCTTCATGGCCTTCAACCGAGCTGCCTCCTTCCGGCCAGG
  CCTGGTTTCTGAGACCCTCAGTGTCCGTACCTTCCACTTCATTGAGCAGAACCTCACCAACTACTATGAG
  ATGATGCTGACTGACCGCAAGGAAGCTGCCTCCTGGGCACGCCGGATGCACTTGGCTCTGAAGGCCTATC
  AGGAGCTGCTGGCAACAGTGAATGAGATGGACATATCTCCAGATGAGGCTGTGAGGGAGAGCAGCCGCAT
  CATCAAGAACAATATTTTCTATGTGATGGAGTACCGAGAACTATTCCTGGCACTTTTTCGAAAGTTTGAT
  GAGAGATGCCAGCCCCGCTCTTTCCTTCGTGACCTGGTGGAGACCACCCACCTCTTCCTCAAAATGTTGG
  AGCGATTCTGTCGGAGCCGTGGGAACCTGGTGGTGCAGAACAAACAAAAGAAGAGAAGGAAGAAGAAGAA
  GAAGGTCCTAGACCAGGCCATTGTTTCTGGTAATGTCCCATCTAGCCCAGAAGAAGTGGAGGCTGTGTGG
  CCAGCCCTGGCTGAGCAGCTACAGTGCTGTGCCCAGAATTCTGAGCTCAGCATGGACTCCGTGGTTCCCT
  TTGATGCGGCCTCAGAGGTGCCAGTGGAGGAGCAGCGGGCAGAAGCTATGGTACGGATCCAAGACTGTCT
  CCTGGCTGGCCAGGCCCCACAGGCCCTGACTCTCCTGAGGTCTGCTCGGGAGGTGTGGCCTGAAGGAGAT
  GTGTTTGGCTCTCAAGACATTTCTCCAGAGGAAGAGATCCAGTTGCTGAAACAAATCCTCTCTGCTCCAC
  TTCCCCGGCAGCAGGGCCCAGAGGAACGTGGGGCAGAGGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGA
  GGAGTTGCAAGTGGTCCAGGTGTCGGAGAAAGAATTTAATTTTCTGGACTACCTGAAACGCTTTGCATGT
  TCAACTGTCGTTCGAGCCTATGTGCTGCTACTAAGGAGCTACCAGCAGAATAGTGCCCACACTAACCATT
  GCATTGTGAAGATGCTGCACCGGCTGGCCCATGACCTCAAAATGGAAGCCCTACTTTTTCAGCTGTCAGT
  CTTCTGCCTCTTCAATCGTCTGCTTAGTGACCCTGCTGCTGGAGCCTACAAAGAGCTAGTGACTTTTGCC
  AAATACATCCTGGGCAAATTTTTTGCACTGGCTGCAGTCAACCAAAAAGCCTTTGTGGAGCTGTTGTTCT
  GGAAGAACACAGCTGTGGTTCGAGAGATGACTGAGGGCTATGGCTCCCTGGATGACAGGTCTTCCAGTCG
  CAGAGCACCTACATGGAGCCCCGAAGAAGAGGCTCATCTTCGGGAGCTGTACCTCGCCAATAAGGACGTG
  GAAGGGCAGGATGTGGTGGAAGCCATCTTGGCCCACCTGAATACTGTTCCTCGAACACGCAAGCAGATCA
  TCCACCATCTGGTACAGATGGGACTGGCTGACAGTGTCAAGGACTTCCAAAGGAAAGGAACCCATATTGT
  ACTGTGGACGGGGGATCAGGAGTTGGAGCTGCAGCGGCTTTTTGAGGAATTCCGGGACTCAGATGATGTC
  CTGGGTCATATCATGAAGAATATCACAGCCAAACGCTCACGGGCCCGAATAGTGGATAAACTCTTGGCTC
  TGGGGCTGGTGGCTGAGCGGCGGGAGCTGTACAAGAAACGGCAGAAAAAGTTGGCATCCTCCATCTTGCC
  AAATGGAGCGGAGTCCCTGAAAGATTTTTGCCAGGAAGATCTGGAAGAAGAGGAAAACCTGCCTGAGGAA
  GACAGCGAAGAGGAAGAAGAAGGGGGCTCAGAAGCAGAACAAGTCCAGGGTAGCTTAGTCCTTTCAAATG
  AAAACCTTGGTCAAAGCCTGCATCAGGAAGGCTTTTCTATCCCGCTCCTATGGCTCCAGAACTGCCTGAT
  CCGAGCAGCTGATGATCGGGAAGAGGATGGCTGCTCCCAGGCCGTTCCATTGGTGCCACTCACAGAGGAA
  AATGAGGAAGCCATGGAAAACGAACAGTTTCAGCAGCTGTTGCGCAAGCTAGGGGTTCGGCCCCCTGCCT
  CTGGGCAGGAAACCTTCTGGCGAATTCCAGCCAAGCTGAGTCCTACCCAGCTCCGGAGGGCAGCAGCTTC
  TTTGAGTCAACCAGAGGAGGAACAGAAGCTGCAGCCAGAGCTGCAGCCTAAAGTCCCTGGAGAGCAAGGC
  TCTGATGAGGAGCACTGTAAAGAGCACCGAGCACAAGCCCTGAGGGCCCTCTTGCTAGCCCACAAGAAGA
  AAGCGGGCCTGGCATCCCCAGAGGAGGAAGACGCTGTTGGTAAAGAGCCGCTGAAGGCAGCACCCAAGAA
  ACGACAATTGCTGGACAGCGACGAGGAACAGGAAGAAGATGAGGGCAGGAACAGAGCACCAGAGTTGGGA
  GCTCCAGGAATCCAAAAGAAGAAACGATACCAGATTGAGGATGATGAGGATGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003920 unedited
  GTATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCAGCTCCTCCGGGC
  TTCTGAGGACCCGCAGGGAGCGGGCCTCATCATTTCTGTCTCCTGTTTCTCCACTTCCTT
  CTCTATTGGTTGGTCCACTGATGTATGGACTTGCACATGATGAACTGTGAACTTCTAGCC
  ACATGTAGTGCCCTTGGGTACTTGGAGGGAGACACTTACCATAAGGAACCAGATTGCTTA
  GAGAGCGTGAAGGATCTGATCCGCTATTTGAGGCATGAGGATGAGACACGAGATGTGCGG
  CAGCAGCTGGGGGCAGCCCAGATCCTACAGAGCGACCTTCTGCCCATCCTCACCCAGCAC
  CACCAGGACAAGCCTCTCTTTGATGCTGTTATCAGACTGATGGTGAACTTGACACAACCA
  GCCTTGCTCTGTTTTGGCAATCTGCCTAAGGAGCCCAGCTTTCGGCACCATTTTTTGCAG
  GTGCTAACTTATTTGCAGGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCTTTTGGAGTC
  CTCAGTGAAACCTTGTATGAGCTGCTGCAGCTGGGCTGGGAGGAACGGCAGGAGGAAGAC
  AACTTGCTGATTGAACGGATCCTACTGCTGGTCAGAAATATTCTCCATGTCCCAGCTGAC
  CTTGATCAGGAGAAGAAGATTGATGATGACGCCAGTGCCCATGACCAGCTCCTCTGGGCG
  ATTCACCTCAGCGGCCTGGATGACCTGCTCCTCTTTCTGGCCAGCTCGTCTGCTGAGGAG
  CAATGGAGCCTACATGTGCTAGAGATTGTCTCCCTTATGTTTCGTGACCAGAAACCCGAG
  CAGCTGTCGGAAGTAGGCCAGGNACGCTTTACTCAGGAGCGNAGTGCAGATTTTGCAGAA
  CTGTNAGTATTGCCCCCCCGAGAGATGGCAGAAAGCAGATCGAGCCTTCCAGCGAGCCAC
  AGGCATTCTCGATTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003920 unedited
  AAGCTTGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTGAAAACTTCTAATCTTACATGTTTAATACCAAACACAAAGCAAAC
  ACATTCATCATTTAAAGTACTATTTTCCAACCCTATTTCAAATGCAGGAATGGGCCTTTA
  TTTGGTTTTTAAAGGATGATCATAGGAAACAAAATGAGGACAGAGGCATTTGGTATTTTT
  TAAAAGGGGTACAAAACTCCCGCCTGTCATGTCATAAAGCTCCCCATTTGGGGCTGGGAC
  CCACCTCCAAAAAAAAGCCCAAGGAACAGACCAATAAAAGGGGTCCGGGGGGCTTTCTCT
  ATTTCACTCACTCCTCTTATTTGCTAAGGAGCTCCAATAACCAAAAAAAATGGTTCCTAA
  AAAAAAAAAACTAAATCTCCAAAAAGCTGCTGGGGCATACCGATAAAAACTGGGAGAAAC
  AAAGACTGACAGCAGAAAAGTCCAATACTGCTGCTGAAGTTTCTCAACCTAAATCCGTGA
  AAGAACCTGGGATTCTACTGCTCTGTCCAATAAGAGGAGCTTCTGGGTCCTGTATGACCC
  TTCCAACTCTGACTTGAATGCACCATCTAAAAACGTGTCTATCTCTACCCCTAAGCTTCT
  TTAACTCTTCAGGCATCCTCATCATCCTCAATCTGGGATCCGTTCTTCTTTTGGATTCCT
  GGAGCTCCAACTCTGGGGCTCTGTTCCTGCCCTATATTCTTCCTGTNCCTCGNGCGTGGC
  AACAATTGTCGTTTCTGGGTGCTGGCTTCAAGGCTTTTTACCAAAGCGTTTTCTTCTTTT
  GGGAATGCAAGCCCGTTTTTTTTTTGGGGCCTACAAAAGGCCTCAAGGCTTGTGCTGGGG
  GCTTTTAAGGGCTCCTCATAAACCTTGCTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003920
• Insert Size: 4860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003920.2, NP_003911.1
• RefSeq Size: 4409 bp
• RefSeq ORF: 3627 bp
• Locus ID: 8914
• UniProt ID: Q9UNS1
• Cytogenetics: 12q13.3
• Domains: TIMELESS, TIMELESS_C
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).