TIMELESS (NM_003920) Human Tagged ORF Clone

CAT#: RC208527

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TIMELESS (Myc-DDK-tagged)-Human timeless homolog (Drosophila) (TIMELESS)



  "NM_003920" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,439.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TIMELESS
Synonyms hTIM; TIM; TIM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTTGCACATGATGAACTGTGAACTTCTAGCCACATGTAGTGCCCTTGGGTACTTGGAGGGAGACA
CTTACCATAAGGAACCAGATTGCTTAGAGAGCGTGAAGGATCTCATCCGCTATTTGAGGCATGAGGATGA
GACACGAGATGTGCGGCAGCAGCTGGGGGCAGCCCAGATCCTACAGAGCGACCTTCTGCCCATCCTCACC
CAGCACCACCAGGACAAGCCTCTCTTTGATGCTGTTATCAGACTGATGGTGAACTTGACACAACCAGCCT
TGCTCTGTTTTGGCAATCTGCCTAAGGAGCCCAGCTTTCGGCACCATTTTTTGCAGGTGCTAACTTATTT
GCAGGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCTTTTGGAGTCCTCAGTGAAACCTTGTATGAGCTG
CTGCAGCTGGGCTGGGAGGAACGGCAGGAGGAAGACAACTTGCTGATTGAACGGATCCTACTGCTGGTCA
GAAATATTCTCCATGTCCCAGCTGACCTTGATCAGGAGAAGAAGATTGATGATGACGCCAGTGCCCATGA
CCAGCTCCTCTGGGCGATTCACCTCAGCGGCCTGGATGACCTGCTCCTCTTTCTGGCCAGCTCGTCTGCT
GAGGAGCAATGGAGCCTACATGTGCTAGAGATTGTCTCCCTTATGTTTCGTGACCAGAACCCCGAGCAGC
TGGCGGGAGTAGGGCAGGGACGCTTAGCTCAGGAGCGGAGTGCAGATTTTGCAGAACTGGAGGTGTTGCG
CCAGCGAGAGATGGCAGAAAAGAAGACTCGAGCCCTCCAGCGAGGCAACAGGCATTCTCGATTTGGGGGC
TCCTATATTGTCCAGGGGTTGAAATCCATTGGGGAGAGGGACCTCATCTTTCACAAAGGCCTTCACAACC
TACGAAACTACAGTTCAGATTTGGGAAAGCAGCCGAAAAAGGTGCCTAAACGTCGCCAGGCCGCCCGAGA
GCTGTCCATTCAGCGCCGTTCTGCCCTCAATGTGAGGCTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG
GAGAACTGTTACAACCGGCTCATGGGATCAGTAAAGGATCACCTGCTTCGGGAGAAAGCTCAGCAGCATG
ATGAGACCTATTATATGTGGGCCTTGGCTTTCTTCATGGCCTTCAACCGAGCTGCCTCCTTCCGGCCAGG
CCTGGTTTCTGAGACCCTCAGTGTCCGTACCTTCCACTTCATTGAGCAGAACCTCACCAACTACTATGAG
ATGATGCTGACTGACCGCAAGGAAGCTGCCTCCTGGGCACGCCGGATGCACTTGGCTCTGAAGGCCTATC
AGGAGCTGCTGGCAACAGTGAATGAGATGGACATATCTCCAGATGAGGCTGTGAGGGAGAGCAGCCGCAT
CATCAAGAACAATATTTTCTATGTGATGGAGTACCGAGAACTATTCCTGGCACTTTTTCGAAAGTTTGAT
GAGAGATGCCAGCCCCGCTCTTTCCTTCGTGACCTGGTGGAGACCACCCACCTCTTCCTCAAAATGTTGG
AGCGATTCTGTCGGAGCCGTGGGAACCTGGTGGTGCAGAACAAACAAAAGAAGAGAAGGAAGAAGAAGAA
GAAGGTCCTAGACCAGGCCATTGTTTCTGGTAATGTCCCATCTAGCCCAGAAGAAGTGGAGGCTGTGTGG
CCAGCCCTGGCTGAGCAGCTACAGTGCTGTGCCCAGAATTCTGAGCTCAGCATGGACTCCGTGGTTCCCT
TTGATGCGGCCTCAGAGGTGCCAGTGGAGGAGCAGCGGGCAGAAGCTATGGTACGGATCCAAGACTGTCT
CCTGGCTGGCCAGGCCCCACAGGCCCTGACTCTCCTGAGGTCTGCTCGGGAGGTGTGGCCTGAAGGAGAT
GTGTTTGGCTCTCAAGACATTTCTCCAGAGGAAGAGATCCAGTTGCTGAAACAAATCCTCTCTGCTCCAC
TTCCCCGGCAGCAGGGCCCAGAGGAACGTGGGGCAGAGGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGA
GGAGTTGCAAGTGGTCCAGGTGTCGGAGAAAGAATTTAATTTTCTGGACTACCTGAAACGCTTTGCATGT
TCAACTGTCGTTCGAGCCTATGTGCTGCTACTAAGGAGCTACCAGCAGAATAGTGCCCACACTAACCATT
GCATTGTGAAGATGCTGCACCGGCTGGCCCATGACCTCAAAATGGAAGCCCTACTTTTTCAGCTGTCAGT
CTTCTGCCTCTTCAATCGTCTGCTTAGTGACCCTGCTGCTGGAGCCTACAAAGAGCTAGTGACTTTTGCC
AAATACATCCTGGGCAAATTTTTTGCACTGGCTGCAGTCAACCAAAAAGCCTTTGTGGAGCTGTTGTTCT
GGAAGAACACAGCTGTGGTTCGAGAGATGACTGAGGGCTATGGCTCCCTGGATGACAGGTCTTCCAGTCG
CAGAGCACCTACATGGAGCCCCGAAGAAGAGGCTCATCTTCGGGAGCTGTACCTCGCCAATAAGGACGTG
GAAGGGCAGGATGTGGTGGAAGCCATCTTGGCCCACCTGAATACTGTTCCTCGAACACGCAAGCAGATCA
TCCACCATCTGGTACAGATGGGACTGGCTGACAGTGTCAAGGACTTCCAAAGGAAAGGAACCCATATTGT
ACTGTGGACGGGGGATCAGGAGTTGGAGCTGCAGCGGCTTTTTGAGGAATTCCGGGACTCAGATGATGTC
CTGGGTCATATCATGAAGAATATCACAGCCAAACGCTCACGGGCCCGAATAGTGGATAAACTCTTGGCTC
TGGGGCTGGTGGCTGAGCGGCGGGAGCTGTACAAGAAACGGCAGAAAAAGTTGGCATCCTCCATCTTGCC
AAATGGAGCGGAGTCCCTGAAAGATTTTTGCCAGGAAGATCTGGAAGAAGAGGAAAACCTGCCTGAGGAA
GACAGCGAAGAGGAAGAAGAAGGGGGCTCAGAAGCAGAACAAGTCCAGGGTAGCTTAGTCCTTTCAAATG
AAAACCTTGGTCAAAGCCTGCATCAGGAAGGCTTTTCTATCCTGCTCCTATGGCTCCAGAACTGCCTGAT
CCGAGCAGCTGATGATCGGGAAGAGGATGGCTGCTCCCAGGCCGTTCCATTGGTGCCACTCACAGAGGAA
AATGAGGAAGCCATGGAAAACGAACAGTTTCAGCAGCTGTTGCGCAAGCTAGGGGTTCGGCCCCCTGCCT
CTGGGCAGGAAACCTTCTGGCGAATTCCAGCCAAGCTGAGTCCTACCCAGCTCCGGAGGGCAGCAGCTTC
TTTGAGTCAACCAGAGGAGGAACAGAAGCTGCAGCCAGAGCTGCAGCCTAAAGTCCCTGGAGAGCAAGGC
TCTGATGAGGAGCACTGTAAAGAGCACCGAGCACAAGCCCTGAGGGCCCTCTTGCTAGCCCACAAGAAGA
AAGCGGGCCTGGCATCCCCAGAGGAGGAAGACGCTGTTGGTAAAGAGCCGCTGAAGGCAGCACCCAAGAA
ACGACAATTGCTGGACAGCGACGAGGAACAGGAAGAAGATGAGGGCAGGAACAGAGCACCAGAGTTGGGA
GCTCCAGGAATCCAAAAGAAGAAACGATACCAGATTGAGGATGATGAGGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208527 protein sequence
Red=Cloning site Green=Tags(s)

MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILT
QHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPSFRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYEL
LQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKKIDDDASAHDQLLWAIHLSGLDDLLLFLASSSA
EEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQERSADFAELEVLRQREMAEKKTRALQRGNRHSRFGG
SYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQPKKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFL
ENCYNRLMGSVKDHLLREKAQQHDETYYMWALAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYE
MMLTDRKEAASWARRMHLALKAYQELLATVNEMDISPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFD
ERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVVQNKQKKRRKKKKKVLDQAIVSGNVPSSPEEVEAVW
PALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGD
VFGSQDISPEEEIQLLKQILSAPLPRQQGPEERGAEEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFAC
STVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFA
KYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLRELYLANKDV
EGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDV
LGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEE
DSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSILLLWLQNCLIRAADDREEDGCSQAVPLVPLTEE
NEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQG
SDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELG
APGIQKKKRYQIEDDEDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003920
ORF Size 3624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003920.5
RefSeq Size 5154 bp
RefSeq ORF 3627 bp
Locus ID 8914
UniProt ID Q9UNS1
Cytogenetics 12q13.3
Domains TIMELESS, TIMELESS_C
Protein Families Transcription Factors
MW 138.7 kDa
Gene Summary The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014]

Other Versions

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