HDGF (NM_004494) Human Untagged Clone

CAT#: SC117340

HDGF (untagged)-Human hepatoma-derived growth factor (HDGF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HDGF
• Synonyms: HMG1L2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004494, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGCGATCCAACCGGCAGAAGGAGTACAAATGCGGGGACCTGGTGTTCGCCAAGATGAAGGGCTACC
  CACACTGGCCGGCCCGGATTGACGAGATGCCTGAGGCTGCCGTGAAATCAACAGCCAACAAATACCAAGT
  CTTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCCAAAGACCTCTTCCCTTACGAGGAATCCAAG
  GAGAAGTTTGGCAAGCCCAACAAGAGGAAAGGGTTCAGCGAGGGGCTGTGGGAGATCGAGAACAACCCTA
  CTGTCAAGGCTTCCGGCTATCAGTCCTCCCAGAAAAAGAGCTGTGTGGAAGAGCCTGAACCAGAGCCCGA
  AGCTGCAGAGGGTGACGGTGATAAGAAGGGGAATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTC
  ATTGATGAGCCAGCCAAGGAGAAGAACGAGAAAGGAGCGTTGAAGAGGAGAGCAGGGGACTTGCTGGAGG
  ACTCTCCTAAACGTCCCAAGGAGGCAGAAAACCCTGAAGGAGAGGAGAAGGAGGCAGCCACCTTGGAGGT
  TGAGAGGCCCCTTCCTATGGAGGTGGAAAAGAATAGCACCCCCTCTGAGCCCGGCTCTGGCCGGGGGCCT
  CCCCAAGAGGAAGAAGAAGAGGAGGATGAAGAGGAAGAGGCTACCAAGGAAGATGCTGAGGCCCCAGGCA
  TCAGAGATCATGAGAGCCTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004494 unedited
  GATTTTGTACTACGATTCACTATAGGGCGGCCGCACAATTCGCACGAGGGCACGAGCGCG
  CACCCACCGCGCCGGAGCCTTGCCCCGATCCGCGCCCGCCCCGTCCGTGCGGCGCGCGGG
  CGGAGACGCCGTGGCCGCGCCGGAGCTCGGGCCGGGGGCCACCATCGAGGCGGGGGCCGC
  GCGAGGGCCGGAGCGGAGCGGCGCCGCCACCGCCGCACGCGCAAACTTGGGCTCGCGCTT
  CCCGGCCCGGCGCGGAGCCCGGGGCGCCCGGAGCCCCGCCATGTCGCGATCCAACCGGCA
  GAAGGAGTACAAATGCGGGGACCTGGTGTTCGCCAAGATGAAGGGCTACCCACACTGGCC
  GGCCCGGATTGACGAGATGCCTGAGGCTGCCGTGAAATCAACAGCCAACAAATACCAAGT
  CTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCCAAAGACCTCTTCCCTTACGAG
  GAATCCAAGGAGAAGTTTGGCAAGCCCAACAAGAGGAAAGGGTTCAGCGAGGGGCTGTGG
  GAGATCGAGAACAACCCTACTGTCAAGGCTTCCGGCTATCAGTCCTCCCAGAAAAAGAGC
  TGTGTGGAAGAGCCTGAACCAGAGCCCGAAGCTGCAGAGGGTGACGGTGATAAGAAGGGG
  AATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTCATTGATGAGCCAGCCAAGGAG
  AAGAACGAGAAAGGAGCGTTGAAGAAGAGAGCAGGGGACTTGCTGGAGGACTCTCCTAAA
  CGTCCCAAGGAGGCAGAAAACCCTGAAAGGAGAGAGAAGGAGGGCAGCCACTTGGNAGGT
  TGAGAAGCCCCCTTNCTATGGGAGGTGGAAAAGAATAAGCACCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004494 unedited
  AATTTANAGTCGAGTTTTTTTTTTTTTTTTTTAGTAATCAACGGGTTTTATTTTCCTAGA
  ACTGAAATCATCTACGGTTCTCAGAGCTAAACTTCCAAAGCTACAGTCAGCAATTTTTCA
  TCAGAGCCCAAGGGAGAGGGGCCAGGGTAAAAGAGACGAGACTGTAGAGAGGCATAGAGA
  GACCACTAGGAAGAGGGTGGGAGAGGGCACTTATTTCTCTCTGTCCTCTCAGTGGGTTAC
  AAATCAGATCTGGTGACAACACTGAGGGGGCCAGGTCAGGGTATGTGGATGAGAAATGAC
  ACTGGAAGGAACATCAAAGCCCCAGCTACAAAAAGAAAGTCATCAAGCCCCAAATAGAAG
  GGGAGCCTCCCAGTGCACCTCAGAAATGGGGGCACCGATGGGGAAGGAGCACAATGGAGA
  GCACACAAAGGGTTAGGGGTCTTTAAAATTTTTTTTTGTAATTTTCTTTTATTAAAAACA
  TTTCCTAAAAAATGTGTCTTTTTCCTTTATGTCTGGGTGATATATATGACTATAAGCTGG
  CCTTGAGCACTGTGTGGAGAGGAAAAAGGACAGCTAGGAGTCTTCCCAAGCTTCTATTTG
  GGCCTTACAATCTAAAGGGGGACGATGCTCACACTCCCACCCAAATGGTTAAGTCAACCC
  TTAGGTCTAAGTTCTTGGCCCAAAAACTTGAAGCCCCAATTTCTTGAGATAACTACACCT
  CCAAATTCTGGGTCGTAAAAGAAAAAGGGGTTGCTATCTTAGGATTTTGGAAGAAGCCTC
  TCCTAAAACGGTCCCATTTCCTGTATTTAGCCCCTGGATTTTGGGGTTCAGGGGCAAGGC
  CTCTAAGGAGTCTCCCTTCTGTTGTCCCACCCCCTTTGGGAGTTTCCTTCCCGGCACCAA
  CCCCCCTCTGTTAGTCTCCCTTTTCTTCTATCCGGCCCACCAAGTATTTTTTTTCATCTC
  TCACTGGTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004494
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004494.1, NP_004485.1
• RefSeq Size: 2376 bp
• RefSeq ORF: 723 bp
• Locus ID: 3068
• UniProt ID: P51858
• Cytogenetics: 1q23.1
• Domains: PWWP
• Gene Summary: This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction compared to variant 4. The resulting isoform (a) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform d.