PFKFB3 (NM_004566) Human Untagged Clone

SKU
SC117283
PFKFB3 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1
$485.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PFKFB3
Synonyms iPFK-2; IPFK2; PFK2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117283 sequence for NM_004566 edited (data generated by NextGen Sequencing)
ATGCCGTTGGAACTGACGCAGAGCCGAGTGCAGAAGATCTGGGTGCCCGTGGACCACAGG
CCCTCGTTGCCCAGATCCTGTGGGCCAAAGCTGACCAACTCCCCCACCGTCATCGTCATG
GTGGGCCTCCCCGCCCGGGGCAAGACCTACATCTCCAAGAAGCTGACTCGCTACCTCAAC
TGGATTGGCGTCCCCACAAAAGTGTTCAACGTCGGGGAGTATCGCCGGGAGGCTGTGAAG
CAGTACAGCTCCTACAACTTCTTCCGCCCCGACAATGAGGAAGCCATGAAAGTCCGGAAG
CAATGTGCCTTAGCTGCCTTGAGAGATGTCAAAAGCTACCTGGCGAAAGAAGGGGGACAA
ATTGCGGTTTTCGATGCCACCAATACTACTAGAGAGAGGAGACACATGATCCTTCATTTT
GCCAAAGAAAATGACTTTAAGGCGTTTTTCATCGAGTCGGTGTGCGACGACCCTACAGTT
GTGGCCTCCAATATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCG
GCAGAAGCCATGGACGACTTCATGAAGAGGATCAGTTGCTATGAAGCCAGCTACCAGCCC
CTCGACCCCGACAAATGCGACAGGGACTTGTCGCTGATCAAGGTGATTGACGTGGGCCGG
AGGTTCCTGGTGAACCGGGTGCAGGACCACATCCAGAGCCGCATCGTGTACTACCTGATG
AACATCCACGTGCAGCCGCGTACCATCTACCTGTGCCGGCACGGCGAGAACGAGCACAAC
CTCCAGGGCCGCATCGGGGGCGACTCAGGCCTGTCCAGCCGGGGCAAGAAGTTTGCCAGT
GCTCTGAGCAAGTTCGTGGAGGAGCAGAACCTGAAGGACCTGCGCGTGTGGACCAGCCAG
CTGAAGAGCACCATCCAGACGGCCGAGGCGCTGCGGCTGCCCTACGAGCAGTGGAAGGCG
CTCAATGAGATCGACGCGGGCGTCTGTGAGGAGCTGACCTACGAGGAGATCAGGGACACC
TACCCTGAGGAGTATGCGCTGCGGGAGCAGGACAAGTACTATTACCGCTACCCCACCGGG
GAGTCCTACCAGGACCTGGTCCAGCGCTTGGAGCCAGTGATCATGGAGCTGGAGCGGCAG
GAGAATGTGCTGGTCATCTGCCACCAGGCCGTCCTGCGCTGCCTGCTTGCCTACTTCCTG
GATAAGAGTGCAGAGGAGATGCCCTACCTGAAATGCCCTCTTCACACCGTCCTGAAACTG
ACGCCTGTCGCTTATGGCTGCCGTGTGGAATCCATCTACCTGAACGTGGAGTCCGTCTGC
ACACACCGGGAGAGGTCAGAGGATGCAAAGAAGGGACCTAACCCGCTCATGAGACGCAAT
AGTGTCACCCCGCTAGCCAGCCCCGAACCCACCAAAAAGCCTCGCATCAACAGCTTTGAG
GAGCATGTGGCCTCCACCTCGGCCGCCCTGCCCAGCTGCCTGCCCCCGGAGGTGCCCACG
CAGCTGCCTGGACAAAACATGAAAGGCTCCCGGAGCAGCGCTGACTCCTCCAGGAAACAC
TGA

Clone variation with respect to NM_004566.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004566 unedited
NAAGGGTCAGATATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACCAGCCGC
TCTCCTGCCAGCGTCGGGATCTCGGCCCCGGGAGGCGGGCCGTCGGGCGCAGCCGCGAAG
ATGCCGTTGGAACTGACGCAGAGCCGAGTGCAGAAGATCTGGGTGCCCGTGGACCACAGG
CCCTCGTTGCCCAGATCCTGTGGGCCAAAGCTGACCAACTCCCCCACCGTCATCGTCATG
GTGGGCCTCCCCGCCCGGGGCAAGACCTACATCTCCAAGAAGCTGACTCGCTACCTCAAC
TGGATTGGCGTCCCCACAAAAGTGTTCAACGTCGGGGAGTATCGCCGGGAGGCTGTGAAG
CAGTACAGCTCCTACAACTTCTTCCGCCCCGACAATGAGGAAGCCATGAAAGTCCGGAAG
CAATGTGCCTTAGCTGCCTTGAGAGATGTCAAAAGCTACCTGGCGAAAGAAGGGGGACAA
ATTGCGGTTTTCGATGCCACCAATACTACTAGAGAGAGGAGACACATGATCCTTCATTTT
GCCAAAGAAAATGACTTTAAGGCGTTTTTCATCGAGTCGGTGTGCGACGACCCTACAGTT
GTGGCCTCCAATATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCG
GCAGAAGCCATGGACGACTTCATGAAGAGGATCAGTTGCTATGAAGCCAGCTACCAGCCC
CTCGACCCCGACAAATGCGACAGGGACTTGTCGCTGATCAAGGTGATTGACGTGGGCCGG
AGGCTCCTGGTGAACCGGGTGCAGGACCACATCCAGAGCCGCATCGTGTACTACCTGATG
AAATNCAGTGCAGCCGCGTCCATCTACCTGTGCCGGCACGGCGAGAACGAGCACACCTTC
CAGGCCGATCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004566 unedited
NNNNNNNTTTGGTACATCTAAGNACCGCGGNCCGCAANCTAGNGATCGGTTTTTTTTTTT
TTTTTTTTTTGTATTTCAACCAGGTATTTTATTGGTCTGGCAACTGCAAAATATACAAAT
TTCTGAAAGGCATCTCCTGTTTGAAAGCTAGCATAGCTTTATATCCCAGGTCATTAATTC
AGACAAATACACAGAACACAGAGAAGTCATAAGTCACACATAGGAGGCTCCCAAACCAAT
TAACAATGAAACCCAATCTCTACAGCTTTCTATTGTTTAGGCTCCTTATACATATTAAAT
AAATAAATAGCACATCTGCTATCAAAATAATAAAAAAATAAATTTCAAGTATTACAAAGA
AAAATATCATTGGTGTGGTTCCAAACAGTTTTTCTTCTCTCTCAAAATGAAGAGCTTTGC
CCGTGGTCTCAGGACAGAATAAGGAGGGGGGAAACTCACCCAAAAACAAATAGGCAAAGT
TAAAAATATTGGTCCCCGTTATTTTGTGGGGTGGAGAGTTGGAGGAATCAGTAGACGCCA
AAAGTTCATGACTGTAGCTCAAACCCAACCCCCGCCCCCGGCCCCCGTCCAATGTCCCCT
CAAAAGGCAGGTCACTGGCTTGCAAAGTAGGCAGTGTTCTCCTTTCTATAGTATCTCCCT
CCCTGTCCAGATTCATGTTACCCTCTCTCCTATCTAGGCCCTTCGCAAACTAAAAGTCCC
GTGTTAGACCAAGGGCTTCTGTATTTAGGTATTGATTTTAGGTAGTCAGAGGGGGAGAGG
GACCACAGCTATCAAGCCCCTATCTGTATCAAACTTCAGAGTTGATGTTCAAAGAATGAN
GCCTGCGAGGGGGACACGAGNAACTATTCTCAAGAGCAAGCACN
Restriction Sites NotI-NotI
ACCN NM_004566
Insert Size 4260 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004566.2, NP_004557.1
RefSeq Size 4497 bp
RefSeq ORF 1563 bp
Locus ID 5209
UniProt ID Q16875
Cytogenetics 10p15.1
Domains 6PF2K, PGAM
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism
Summary The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Citations

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