SNAP29 (NM_004782) Human Untagged Clone

SKU SC117140

SNAP29 (untagged)-Human synaptosomal-associated protein, 29kDa (SNAP29)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SNAP29
• Synonyms: CEDNIK; SNAP-29
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004782 edited; GAATTCGGCACGAGGCCGGCACCATGTCAGCTTACCCTAAAAGCTACAATCCGTTCGACG; ACGACGGGGAGGACGAAGGCGCCCGGCCGGCCCCTTGGAGGGACGCCCGAGACCTCCCCG; ACGGGCCCGACGCGCCCGCGGACAGGCAGCAGTACTTGCGGCAGGAGGTCCTCCGCAGGG; CTGAGGCCACGGCCGCCAGCACCAGCAGGTCCCTGGCCCTCATGTACGAGTCCGAGAAGG; TTGGGGTCGCCTCTTCCGAGGAGCTCGCCCGTCAGCGAGGAGTCCTGGAGCGCACAGAGA; AGATGGTGGACAAGATGGACCAAGATTTGAAGATCAGCCAGAAACACATCAATAGCATTA; AGAGCGTGTTTGGGGGGCTGGTCAATTACTTCAAATCCAAACCAGTAGAGACCCCACCTG; AACAGAATGGCACCCTCACCTCCCAGCCCAACAACAGATTGAAAGAAGCTATAAGTACAA; GTAAAGAACAGGAAGCAAAGTACCAGGCCAGCCACCCAAACCTTAGAAAGCTGGATGATA; CAGACCCTGTCCCCAGAGGGGCTGGTTCTGCCATGAGTACTGATGCTTACCCAAAGAACC; CACACCTTCGAGCCTATCACCAGAAGATCGACAGCAACCTAGATGAGCTGTCCATGGGAC; TGGGTCGTCTGAAGGACATAGCCCTGGGGATGCAGACAGAAATTGAGGAGCAAGATGACA; TTCTTGACCGGCTGACAACCAAAGTGGACAAGTTAGATGTCAACATAAAAAGCACAGAAA; GAAAAGTTCGACAACTCTGAAGACAGACGGATTTCCACTCTATTGTGATGAAAAGATTTG; AAAGATCTTTTTTTGAACTTCCAAGAAATTTCATTTACTATTTTAGTATGTAAATTAATG; TGTGTTTGCAAATGTTATAATAGAGTAGGTCTTAAGACATTTTTGCTGTTATAAGGAAGT; GTTTGTCCCACATTTTCCTAGGGTTAACACCTCACCAAGTTCTTCCAGCAAAATGCTTAT; TAGAGTTTTTGTCTGAGCACAGTAGCCTGGCCCTGCATATCTTGGGCGTTTGATTACCAT; GCTGGAATAAGAAGAGTTGCATTTCTCATTTTAATGAGCATATGGAAAGATGGGAACAGA; ATTTCAGTCTTGCAGCCATGAGCCTCTATTTCTCATTCCTAAGCCTTACCATGAGTCAGA; GTGTTAAGGGGGCCTGTGAGCCAGTCGTTTGGTGGAGGAGGGTCCTTCCCACACCTTTGT; TTAGGAGTCATCATTCACACACACACACACACACACACACACACACACACACACTCTGAG; CACATTATCTGTGATTCTTTCATCCTCCCAGAAAGGATCAGTGTTAGAATGGGAAATAGA; GTGTGCCATCTTGCTCTTTTGTTGGCAGATGTCCCCGCTTCCCTCCAGCCCCCCATCCTT; GGCATGTATATTAGTAGGAGGGCTTCACACCAGCACTGCTCACTGCGACTCCTGGAAATG; TGTGGGGAATTGGGTGAGCCCCTCTTCCCCACGTGCTCCCATCTGTAACTCTCCATCTGT; GATTTGCAACAGGTCTTCCACTGGCTCGAGGCTGCGAGGCTGACCGACAGGGGCGCCCAA; TAGAGTTTGTGGGAATGGAGTATTGCACAGATGCAGCTGCACAATGGTCTTCCTCCACCC; CTCTGGGAATCACCATTAGGCAGGCCTCAGTAGGGCAAAATGTTTATTTGGTACCGGGGG; TCTCCTGACCTGTCTAGCATTCCCACCCACTCTTCTGCACTTCTTGCAAGGTAACTTGAA; TCATCACTTATTTCTCTGCCACCTGGTTTTAGGACTGGCACTCTATAAATGACGCTGACT; TTGTATAGAAGGATTTTGCTAAGGGGGCAAAAAGCCCAGATACCATTTTAACTGGCCCAG; CTCATTTTCTCCTTTCATTTTACAAAAGAAATCATGCAGAAGCATCAATTAAAACTGATT; TATTTATGTTGGTACATCCAACAAAATAGTTGGAGCCACAAGGCCCTTGTCTTTTTAAAA; AAACAAAATATTTTGAAGATGGTGAAAGTGACTGATTTTAATAATGAGATAGGTCACTTA; AAATAACAGTGCCTTTGACCTTAAAACATGGCACTGGCCCATGTGACCTTTGCCATTTCC; CAGTACTTCCCTTTACAGTCTGCAGCCTATAAACAGTGGTGTCTCAAAGCAAAGGAAACC; TCCACAAGTGCTGCAACAGTGCATTAGTTTTTATGTAGCAATTTGTTTATAAAAATTCCC; ATCCCATAATGCATGACCTTCCTAAAAATACCCACAGCCTCCATAGGCAAGGTGAGCTTT; CTGTGCTACCCACTGGTTAGATTCCAGCCACCTCAGGGATTCTGATCCATGTCATTAACG; ACCATCCCAGGGTGTGAGGGCCTGATTAAAGCTTATCGAACTAGACATTTGTTTATTAGA; TGAGCTCGGCATTATTATTTTCTCAATAACTGGTAGAAGGGAAATTTTAAACATAACACT; CAACCCAAACAAAACTACTTCGTGAGGCTTTCAGCAAAAAGGCTGCTTAAAAGCATGAAC; CCGGGATGCAGAGCAGCTTTTGTGCACGGTTCCACTTACTGTAATTGTTTCTGGTTCTTT; CCTCTAACACCTTCCATGTATGTCAACTTTTTATCACCGTCAGGCATAGACTCGTGGAGA; TTAGTCATTATAGCTCAGACCTAAGCTAATTATTTAAAATAAATACATGTAAAGGCCACT; GCCAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004782 unedited; ACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGCACCATGTCAGCTTACCCTAAAAGC; TACAATCCGTTCGACGACGACGGGGAGGACGAAGGCGCCCGGCCGGCCCCTTGGAGGGAC; GCCCGAGACCTCCCCGACGGGCCCGACGCGCCCGCGGACAGGCAGCAGTACTTGCGGCAG; GAGGTCCTCCGCAGGGCTGAGGCCACGGCCGCCAGCACCAGCAGGTCCCTGGCCCTCATG; TACGAGTCCGAGAAGGTTGGGGTCGCCTCTTCCGAGGAGCTCGCCCGTCAGCGAGGAGTC; CTGGAGCGCACAGAGAAGATGGTGGACAAGATGGACCAAGATTTGAAGATCAGCCAGAAA; CACATCAATAGCATTAAGAGCGTGTTTGGGGGGCTGGTCAATTACTTCAAATCCAAACCA; GTAGAGACCCCACCTGAACAGAATGGCACCCTCACCTCCCAGCCCAACAACAGATTGAAA; GAAGCCTATAGTACAAGTAAAGAACAGGAAGCANAGTACCAGGCCAGCCACCCANACCTT; AGAAAGCTGGATGATACAGACCCTGTCCCCAGAGGGGCTGGNTCTGCCATGAGTACTGAT; GCTTACCCCAAGACCCACACCTTCGAGCCTATCACCAGAAGATCGACAGNCACCTAGATG; AGCTGTCCATGGGACTGGGTCCGCTGAAGACATAGNCCTGGNGATGCAGACAGAATTGAG; GAGCAAGAGACATCCTGACCGCTGACACCAAGTGAAAGTTAGAGCCACATAAAAGCCACG; AAGAAAGTCGACACTTTGAGACGACGATTCCCTCTATTGGATGAAAGATGGAGACCTTTT; TGACTCCAGAANTCATTACTATTAGATGAACTAGGGGGTCGCAAGTAAAAAAGAGCCTAG; ACTTTGCGTTAAGAAGGGTGCCAATTCCTAGTACCCTCCAGTCTCAAAGTATATAAGTTG; CGACCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004782 unedited; TGTGAACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGCAGTGGCCT; TTACATGTATTTATTTTAAATAATTAGCTTAGGTCTGAGCTATAATGACTAATCTCCACG; AGTCTATGCCTGACGGTGATAAAAAGTTGACATACATGGAAGGTGTTAGAGGAAAGAACC; AGAAACAATTACAGTAAGTGGAACCGTGCACAAAAGCTGCTCTGCATCCCGGGTTCATGC; TTTTAAGCAGCCTTTTTGCTGAAAGCCTCACGAAGTAGTTTTGTTTGGGTTGAGTGTTAT; GTTTAAAATTTCCCTTCTACCAGTTATTGAGAAAATAATAATGCCGAGCTCATCTAATAA; ACAAATGTCTAGTTCGATAAGCTTTAATCAGGCCCTCACACCCTGGGATGGTCGTTAATG; ACATGGATCAGAATCCCTGAGGTGGCTGGAATCTAACCAGTGGGTAGCACAGAAAGCTCA; CCTTGCCTATGGAGGCTGTGGGTATTTTTAGGAAGGTCATGCATTATGGGATGGGAATTT; TTATAAACAAATTGCTACATAAAAACTAATGCACTGTTGCAGCACTTGTGGAGGTTTCCT; TTGCTTTGAGACACCACTGTTTATAGGCTGCAGACTGTAAAGGGAAGTACTGGGAAATGG; CAAAGGTCACATGGGCCAGTGCCATGTTTTAAGGTCAAAGGCACTGTTATTTTAAGTGAC; CTATCTCATTATTAAAATCAGTCACTTTCACCATCNTCAAAATATTTTGTTTTTTTAAAA; AGACAAGGCCTTTGTGCTNCACTATTTTGTTGGATGTACCAACATAATAATCAGTTTAAA; TGATGCTCTGCATGATTNCTTTGTAAATGAAAGAGAANATGAGCTGGCCAGTAAATGGTT; CTGGGCTTTTTGCCCTTAGCAATNCTCTATCAGTCACGTCATTTAGATGCAGTCTAAACA; GTGCAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004782
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004782.2, NP_004773.1
• RefSeq Size: 2863 bp
• RefSeq ORF: 777 bp
• Locus ID: 9342
• UniProt ID: O95721
• Cytogenetics: 22q11.21
• Domains: SNAP-25, t_SNARE
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport
• Summary: This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq, Jul 2008]