Calbindin (CALB1) (NM_004929) Human Untagged Clone

SKU SC117035

CALB1 (untagged)-Human calbindin 1, 28kDa (CALB1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Calbindin
• Synonyms: CALB; D-28K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117035 sequence for NM_004929 edited (data generated by NextGen Sequencing); ATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGATCTGG; CTCCATTTCGACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTTGATC; CAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAAAACT; TTTGTGGATCAGTATGGGCAAAGAGATGATGGAAAAATAGGAATTGTAGAGTTGGCTCAC; GTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGATGCCAGCAGCTGAAGTCCTGT; GAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTTCATAGAAACT; GAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGATGAC; ACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGATGGG; AAGCTGGAATTAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCTTAAA; TTCCAGGGAATCAAAATGTGTGGGAAAGAGTTCAATAAGGCTTTTGAGCTGTATGATCAG; GACGGCAATGGATACATAGATGAAAATGAACTGGATGCTTTACTGAAGGATCTGTGCGAG; AAGAATAAACAGGATCTGGATATTAATAATATTACAACATACAAGAAGAACATAATGGCT; TTGTCGGATGGAGGGAAGCTGTACCGAACGGATCTTGCTCTTATTCTCTGTGCTGGGGAT; AACTAG; ; Clone variation with respect to NM_004929.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004929 unedited; GCGGCACGCGCAATTCGGCACGAGGCTGAGCTGAGCAGCACCGAGGACAGCGCCCGGCAG; CGCCCGCGCCCAGGTCTCCCTCCGCAGCCCTGACTCGCGCACACGCTGAGCTTTTGCTCA; CTCCCCCTCGCGCGGACACAGACACACGCATATTCACACACCCAGACACACACCCCGCTG; TACAATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGAT; CTGGCTCCATTTCGACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTT; GATCCAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAA; AACTTTTGTGGATCAGTATGGGCAAAGAGATGATGGAAAAATAGGAATTGTAGAGTTGGC; TCACGTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGATGCCAGCAGCTGAAGTC; CTGTGAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTTCATAGA; AACTGAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGA; TGACACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGA; TGGGAAGCTGGAATTAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCT; TAATTCCAGGGAATCAAAATGTGTGGGAAAAGAGTTCATAAGGGCTTTGAGCTGTATGAT; CAGGACNNGCATGGATACATAGATGAAAATGAACTGGGATGCTTACTGAAGGATCTGTGC; GAGAAGAATAACA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004929 unedited; GACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTAAGGTTATTTTCT; TCTTTATTTATTCTTAGTAGTTAAATTCAGTCTAATGGCAGTGCTTCCATTTACACGCTA; AAGGCAAAGCCAATCTCCCACTCAACATCATTTGTTGAATTTTAGTTTTTGGCAAATCTC; GTTTCTGTTTGTTCTTGAGGGAAACATTATAGCTCACATTTTTTGGTTATTTTTATGAAT; TCAGAAAATAAGTGTTGAATTTATAGAATGGGGACATTCAAGCTGACCCAGAACGATCAT; TAACTCCTAGGCAGTAACTGTAAATTTCACATGGACACATCTCTCATAGCTCCCTGTTTC; ACAATCAAAGAAATCTAAAATCACCAACTGTTACATATCAACTATAACCAGTATAGGATA; TTCTAGCTACAAATATGTGAAACTGACTTAGATGTATGTTAAAGAATGTTTTAGAATCTT; CTTGCTAGATAAGATAAATGATTACATTAGCATTTCTTTTCAGATGTTATTTTTTTAAAC; TCATTTGAAACTATTTGCAGATAGAAATAGAACTATTTTGAATGCTGATCTCAATATGAA; ATATAGAATCCACAGAATACACAAAAGTATTGATGATATAACATTCAATAATCAGGATGC; TATGAATTTTACATTAAAATTTAATTATGTAGTAAAAAATANGAGTTGTTATAGCTAGAA; AAAAATATTTTCAGAGGCAGCAGATACCCTTGGTGGAANTGAACANGTTCTCATAGAAAG; GAAATCCCAGAAGTATTGCAACTAGACACAGGATATGCAGTATATAAAATTAATCAGAGA; TACACAGGTTTGACATAAACAGCNGGTGACTGAATATTTTGCACACTGAAAACAGTTAGA; AATGGATTAGGTCCTATCTCTGACGAAAATTGTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004929
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004929.2, NP_004920.1
• RefSeq Size: 2531 bp
• RefSeq ORF: 786 bp
• Locus ID: 793
• UniProt ID: P05937
• Cytogenetics: 8q21.3
• Domains: EFh
• Summary: The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]