ERp57 (PDIA3) (NM_005313) Human Untagged Clone

SKU SC116818

PDIA3 (untagged)-Human protein disulfide isomerase family A, member 3 (PDIA3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ERp57
• Synonyms: ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HEL-S-93n; HEL-S-269; HsT17083; P58; PI-PLC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116818 sequence for NM_005313 edited (data generated by NextGen Sequencing); ATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCTTCTTGCCGCGGCCCGCCTCGCCGCTG; CCTCCGACGTGCTAGAACTCACGGACGACAACTTCGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCT; CATGCTCGTCGAGTTCTTCGCTCCCTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCA; GCTACCAGATTAAAAGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATA; AATATGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTATGATGG; ACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGCTTCAGTGCCTCTCAGG; ACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTCTATAGTAGGTTTTTTCGATGATTCAT; TCAGTGAGGCTCACTCCGAGTTCCTAAAAGCAGCCAGCAACTTGAGGGATAACTACCGATTTGCACATAC; GAATGTTGAGTCTCTGGTGAACGAGTATGATGATAATGGAGAGGGTATCATCTTATTTCGTCCTTCACAT; CTCACTAACAAGTTTGAGGACAAGACTGTGGCATATACAGAGCAAAAAATGACCAGTGGCAAAATTAAAA; AGTTTATCCAGGAAAACATTTTTGGTATCTGCCCTCACATGACAGAAGACAATAAAGATTTGATACAGGG; CAAGGACTTACTTATTGCTTACTATGATGTGGACTATGAAAAGAACGCTAAAGGTTCCAACTACTGGAGA; AACAGGGTAATGATGGTGGCAAAGAAATTCCTGGATGCTGGGCACAAACTCAACTTTGCTGTAGCTAGCC; GCAAAACCTTTAGCCATGAACTTTCTGATTTTGGCTTGGAGAGCACTGCTGGAGAGATTCCTGTTGTTGC; TATCAGAACTGCTAAAGGAGAGAAGTTTGTCATGCAGGAGGAGTTCTCGCGTGATGGGAAGGCTCTGGAG; AGGTTCCTGCAGGATTACTTTGATGGCAATCTGAAGAGATACCTGAAGTCTGAACCTATCCCAGAGAGCA; ATGATGGGCCTGTGAAGGTAGTGGTAGCAGAGAATTTTGATGAAATAGTGAATAATGAAAATAAAGATGT; GCTGATTGAATTTTATGCCCCTTGGTGTGGTCATTGTAAGAACCTGGAGCCCAAGTATAAAGAACTTGGC; GAGAAGCTCAGCAAAGACCCAAATATCGTCATAGCCAAGATGGATGCCACAGCCAATGATGTGCCTTCTC; CATATGAAGTCAGAGGTTTTCCTACCATATACTTCTCTCCAGCCAACAAGAAGCTAAATCCAAAGAAATA; TGAAGGTGGCCGTGAATTAAGTGATTTTATTAGCTATCTACAAAGAGAAGCTACAAACCCCCCTGTAATT; CAAGAAGAAAAACCCAAGAAGAAGAAGAAGGCACAGGAGGATCTCTAA; ; Clone variation with respect to NM_005313.4; 46 c=>n;47 t=>n;48 t=>n;49 g=>n;50 c=>n;51 c=>n;52 g=>n;53 c=>n;162 c=>t;1224 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005313 unedited; CGGTGCAGATTTGTATACGACTCACTATAGGCGGCCGCGCAATCGGCACGAGGGCGGGTT; AGTGGTCGCGCGCCCGACCTCCGCAGTCCCAGCCGAGCCGCGACCCTTCCGGCCGTCCCC; ACCCCACCTCGCCGCCATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCT; TCTTGCCGCGGCCCGCCTCGCCGCTGCCTCCGACGTGCTAGAACTCACGGACGACAACTT; CGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCTCATGCTCGTCGAGTTCTTCGCTCC; CTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCAGCTACCAGATTAAA; AGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATAAATA; TGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTA; TGATGGACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGC; TTCAGTGCCTCTCAGGACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTC; TATAGTAGGTTTTTTCGATGATTCATTCAGTGAGGCTCACTCCGAGTTCCTAGAAGCAGC; CAGCAACTTGAGGGATAACTACCGATTTGCACATACCAATGTTGAGTCTCTGGTGAACGA; GTATGATGATCATGGAGCAGGGATCATCTTATTTCGTCCTTCACATCTCACTAACCAAGT; TTGAAGACAAGACTGTGGCATATACCGAGCCACAAATGACCCGTGGCAACATTTAACAGG; TTATCCCGGACAACATTTTTGGTATCTGCCCTCCCTTGACCGAAGACACTTCGATTTGTT; CCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005313 unedited; GAGCTTAAGGGGTCTCATTTTTGTTACTGTTCCAAAGTACAAAAAAAATAGAAAATACCC; CCAACTCAACCCCACCCCCNACAACATTTCCCCCAACACAAATAATTTTTCCCAAAACCA; CAAACATAAACTGGTCCTGGTATTTCCATAAACAGTGCAGCTAAGAAACAGTTTAGAGAA; AATTTAACAGGATTCAGATTATATCCATTCTGTCCTCTCGGCCCTGAGAGGTAATAATTC; CCATATGGGTCCTAGTCCTCCCCAATGGTTTTCCCATCTCTGATGGAAGAGTCCTTTTAC; AAAGTGGTGTTTGGCTACTGCTTTAGAGATCCTCCTGTGCCTTCTTCTTCTTCTTGGGTT; TTTCTTCTTGAATTACAGGGGGGTTTGTAGCTTCTCTTTGTAGATAGCTAATAAAATCAC; TTAATTCACGGCCACCTTCATATTTCTTTGGATTTAGCTTCTTGTTGGCTGGAGAGAAGT; ATATGGTAGGAAAACCTCTGACTTCATATGGAGAAGGCACATCATTGGCTGTGGCATCCA; TCTTGGCTATGACGATATTTGGGTCTTTGCTGAGCTTCTCGCCAAGTTCTTTATACTTGG; GCTCCAGGTTCTTACAATGACCACACCAAGGGGCATAAAATTCAATCAGCACATCTTTAT; TTTCATTATTCACTATTTCATCAAAATTCTCTGCTACCACTACCTTCACAGGCCCATCAT; TGCTCTCTGGGATAGGTTCAGACTTCAGGTATCTCTTCAGATTGCCATCAAAGTAATCCT; GCAGGGACCTCTNCAGAGCCTTNCCATCACGCGAGAACTCCTCCTGCATGACAAACTTTT; CTTCCTTAGCAGTTCTGATAAGCACAACAGGGAATCTCTTCAGCAGTGCTCTTCAAGCCA; AAATCAGAAAGTTCATGGCTTAAGGGGTTTGCGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_005313
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005313.4, NP_005304.3
• RefSeq Size: 3060 bp
• RefSeq ORF: 1518 bp
• Locus ID: 2923
• UniProt ID: P30101
• Cytogenetics: 15q15.3
• Domains: thiored
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation
• Summary: This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]