PHAP1 (ANP32A) (NM_006305) Human Untagged Clone

SKU SC116212

ANP32A (untagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PHAP1
• Synonyms: C15orf1; HPPCn; I1PP2A; LANP; MAPM; PHAP1; PHAPI; PP32
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116212 sequence for NM_006305 edited (data generated by NextGen Sequencing); ATGGAGATGGGCAGACGGATTCATTTAGAGCTGCGGAACAGGACGCCCTCTGATGTGAAA; GAACTTGTCCTGGACAACAGTCGGTCGAATGAAGGCAAACTCGAAGGCCTCACAGATGAA; TTTGAAGAACTGGAATTCTTAAGTACAATCAACGTAGGCCTCACCTCAATCGCAAACTTA; CCAAAGTTAAACAAACTTAAGAAGCTTGAACTAAGCGATAACAGAGTCTCAGGGGGCCTG; GAAGTATTGGCAGAAAAGTGTCCGAACCTCACGCATCTAAATTTAAGTGGCAACAAAATT; AAAGACCTCAGCACAATAGAGCCACTGAAAAAGTTAGAAAACCTCAAGAGCTTAGACCTT; TTCAATTGCGAGGTAACCAACCTGAACGACTACCGAGAAAATGTGTTCAAGCTCCTCCCG; CAACTCACATATCTCGACGGCTATGACCGGGACGACAAGGAGGCCCCTGACTCGGATGCT; GAGGGCTACGTGGAGGGCCTGGATGATGAGGAGGAGGATGAGGATGAGGAGGAGTATGAT; GAAGATGCTCAGGTAGTGGAAGACGAGGAGGACGAGGATGAGGAGGAGGAAGGTGAAGAG; GAGGACGTGAGTGGAGAGGAGGAGGAGGATGAAGAAGGTTATAACGATGGAGAGGTAGAT; GACGAGGAAGATGAAGAAGAGCTTGGTGAAGAAGAAAGGGGTCAGAAGCGAAAACGAGAA; CCTGAAGATGAGGGAGAAGATGATGACTAA; ; Clone variation with respect to NM_006305.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006305 unedited; TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACCANAAACGCGCG; GCCGTGGGTTCGGGGTTTATTGATTGAATTCCGCCGGCGCGGGAGCCTCTGCAGAGAGAG; AGCGCGAGAGATGGAGATGGGCAGACGGATTCATTTAGAGCTGCGGAACAGGACGCCCTC; TGATGTGAAAGAACTTGTCCTGGACAACAGTCGGTCGAATGAAGGCAAACTCGAAGGCCT; CACAGATGAATTTGAAGAACTGGAATTCTTAAGTACAATCAACGTAGGCCTCACCTCAAT; CGCAAACTTACCAAAGTTAAACAAACTTAAGAAGCTTGAACTAAGCGATAACAGAGTCTC; AGGGGGCCTGGAAGTATTGGCAGAAAAGTGTCCGAACCTCACGCATCTAAATTTAAGTGG; CAACAAAATTAAAGACCTCAGCACAATAGAGCCACTGAAAAAGTTAGAAAACCTCAAGAG; CTTAGACCTTTTCAATTGCGAGGTAACCAACCTGAACGACTACCGAGAAAATGTGTTCAA; GCTCCTCCCGCAACTCACATATCTCGACGGCTATGACCGGGACGACAAGGAGGCCCCTGA; CTCGGATGCTGAGGGCTACGTGGAGGGCCTGGATGATGAGGAGGAGGATGAGGATGAGGA; GGAGTATGATGAAGATGCTCANGTAGTGGAAGACANGGAGACCANGNATGANGAGGAGGA; AGGTGAANAGAAGCTTTANTTGGAAAGGAGGAGGAGATNAANAAGGTATAACGATGGNAG; AGTANATGACNAGGNAGATGAANAANAGCTTGGTGAAGAAGAAGGGGGGTCAGAGCGAAA; CGAGAACCCTGAGATGAGGGAGAAGTGATGACCTAGTGGATAACCTATTTGAAAAATCCT; ATGGTGCGGGGGGTTTTCCCCATATNCCTTTCCCCCCCCTCATNCTGCCCCTGAAACTAT; TTTTCTGATGTACGTGCTGTGGAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_006305
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006305.2, NP_006296.1
• RefSeq Size: 1136 bp
• RefSeq ORF: 750 bp
• Locus ID: 8125
• UniProt ID: P39687
• Cytogenetics: 15q23
• Domains: LRR, LRRcap
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Summary: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.[UniProtKB/Swiss-Prot Function]