ARPP19 (NM_006628) Human Untagged Clone

CAT#: SC115993

ARPP19 (untagged)-Human cAMP-regulated phosphoprotein, 19kDa (ARPP19)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARPP19
• Synonyms: ARPP-16; ARPP-19; ARPP16; ENSAL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115993 sequence for NM_006628 edited (data generated by NextGen Sequencing)
  ATGTCTGCGGAAGTCCCCGAGGCAGCCTCCGCGGAGGAGCAGAAGGAAATGGAAGATAAA
  GTGACTAGTCCAGAGAAAGCAGAAGAAGCAAAATTAAAAGCAAGATATCCTCATCTGGGA
  CAAAAGCCTGGAGGTTCAGATTTCTTAAGGAAACGGTTGCAGAAAGGGCAAAAATATTTT
  GATTCTGGGGATTACAACATGGCTAAAGCAAAAATGAAGAACAAGCAACTTCCTACTGCA
  GCTCCGGATAAGACGGAGGTCACTGGTGACCACATTCCCACTCCGCAAGACCTTCCTCAA
  CGGAAGCCGTCCCTTGTTGCTAGCAAGCTGGCTGGCTGA
  
  Clone variation with respect to NM_006628.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006628 unedited
  GTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCATTTTCGCG
  GGCGGAGGCGGCCCGGCGGGCCCTGGGAGAGCTGGGACGGGCGGCGGCCGGGTGGCCTCG
  GCCACCCGCTAATTGCATCTTTTCCCGGCGTCTCGTCTGCAGAGGGAGCACTATGTCTGC
  GGAAGTCCCCGAGGCAGCCTCCGCGGAGGAGCAGAAGGAAATGGAAGATAAAGTGACTAG
  TCCAGAGAAAGCAGAAGAAGCAAAATTAAAAGCAAGATATCCTCATCTGGGACAAAAGCC
  TGGAGGTTCAGATTTCTTAAGGAAACGGTTGCAGAAAGGGCAAAAATATTTTGATTCTGG
  GGATTACAACATGGCTAAAGCAAAAATGAAGAACAAGCAACTTCCTACTGCAGCTCCGGA
  TAAGACGGAGGTCACTGGTGACCACATTCCCACTCCGCAAGACCTTCCTCAACGGAAGCC
  GTCCCTTGTTGCTAGCAAGCTGGCTGGCTGATTAAGAGGCTGAACTGCATGAATCTGCTA
  AATCTCATTATTTCTCCTTAATATGTTACTTATCTACTTTTTATTTCCTTTCATTCACTA
  GTCATTTGAGACTGACAGCTTTGCAGGTAGCAGTAGTGTGTGCTGCTATTGTGGAATATA
  CGTGTGTAGAGTTTTTGATTAGTTTAACAGTGCACTGGTGAAGAGGACATGTTAGAGCAA
  CATAAGTAAACTACTTGAAATAGTTGTATATATTACCTAACTTCTAGTGTAGTACTGGTT
  CTAACAAGTAACCAAGCAGTTTTAANAATTTAATGTTTTGGCTNTCATTACTTCATCTTA
  ATTATAGCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006628
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006628.4, NP_006619.1
• RefSeq Size: 5162 bp
• RefSeq ORF: 339 bp
• Locus ID: 10776
• UniProt ID: P56211
• Cytogenetics: 15q21.2
• Domains: endosulfine
• Protein Families: Druggable Genome
• Gene Summary: The 19-kD cAMP-regulated phosphoprotein plays a role in regulating mitosis by inhibiting protein phosphatase-2A (PP2A; see MIM 176915) (summary by Gharbi-Ayachi et al., 2010 [PubMed 21164014]).[supplied by OMIM, Feb 2011]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the CDS. The encoded isoform (2) is shorter than isoform 1. Both variants 3 and 4 encode isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.