PDE10A (NM_006661) Human Untagged Clone

SKU SC115943

PDE10A (untagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PDE10A
• Synonyms: ADSD2; HSPDE10A; IOLOD; LINC00473; PDE10A19
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001130690 edited; ACTCCGCGGCGTCCCCAGGGCGCCAGCTTCGCCCTCGCCGCCGCGGCCGCGCTGCTCTTC; GGCTCCGACATGGAAGATGGACCTTCTAATAATGCGAGCTGCTTCCGAAGGCTGACCGAG; TGCTTCCTGAGCCCCAGTTTGACAGATGAAAAAGTGAAGGCATATCTTTCTCTTCACCCC; CAGGTATTAGATGAATTTGTATCTGAAAGTGTTAGTGCAGAGACAGTAGAGAAATGGCTG; AAGAGGAAGAACAACAAATCAGAAGATGAATCAGCTCCTAAGGAAGTCAGCAGGTACCAA; GATACGAATATGCAGGGAGTTGTATATGAACTAAACAGCTATATAGAACAACGGTTGGAC; ACAGGAGGAGACAACCAGCTACTCCTCTATGAACTGAGCAGCATCATTAAAATAGCCACA; AAAGCCGATGGATTTGCACTGTATTTCCTTGGAGAGTGCAATAATAGCCTGTGTATATTC; ACGCCACCTGGGATAAAGGAAGGAAAACCCCGCCTCATCCCTGCTGGGCCCATCACTCAG; GGCACCACCGTCTCTGCTTATGTGGCCAAGTCCAGGAAAACACTGCTAGTAGAAGACATC; CTTGGAGATGAACGATTTCCAAGAGGTACTGGACTGGAATCAGGGACTCGTATCCAGTCT; GTTCTTTGCTTACCAATTGTCACTGCAATTGGTGACTTGATTGGTATTCTCGAGCTGTAT; CGGCACTGGGGCAAAGAAGCCTTCTGTCTTAGTCACCAGGAGGTTGCAACAGCAAATCTT; GCCTGGGCTTCAGTAGCAATACATCAGGTGCAGGTATGCAGAGGCCTTGCCAAACAGACA; GAATTGAATGACTTCCTACTCGACGTATCAAAAACATATTTTGATAACATAGTTGCAATA; GATTCTCTACTTGAACACATAATGATATATGCAAAAAACCTGGTGAATGCCGATCGTTGT; GCGCTTTTCCAGGTGGACCATAAGAACAAGGAGTTATATTCAGACCTTTTTGATATTGGA; GAGGAAAAGGAAGGAAAACCTGTCTTCAAGAAGACCAAAGAGATAAGATTTTCAATTGAG; AAAGGAATTGCTGGCCAAGTAGCAAGAACAGGGGAAGTCCTGAACATTCCAGATGCCTAT; GCAGACCCACGCTTTAACAGAGAAGTAGACTTGTACACAGGCTACACCACGCGGAACATC; CTGTGCATGCCCATCGTCAGCCGAGGCAGCGTGATAGGTGTGGTGCAGATGGTCAACAAA; ATCAGTGGCAGTGCCTTCTCTAAAACAGATGAAAACAACTTCAAAATGTTTGCCGTCTTT; TGTGCTTTAGCCTTACACTGTGCTAATATGTATCATAGAATTCGCCACTCAGAGTGCATT; TACCGGGTAACGATGGAAAAGCTGTCCTACCATAGCATTTGTACTTCAGAAGAGTGGCAA; GGTCTCATGCAATTCACCCTTCCCGTGCGTCTCTGCAAAGAAATTGAATTATTCCACTTT; GACATTGGTCCTTTTGAAAACATGTGGCCTGGAATTTTTGTCTACATGGTTCATCGGTCC; TGTGGGACATCCTGCTTTGAGCTTGAAAAGTTGTGTCGTTTTATTATGTCTGTGAAGAAG; AACTATCGGCGGGTTCCTTATCACAACTGGAAGCATGCGGTCACTGTAGCACACTGCATG; TATGCCATACTTCAGAACAATCACACGCTTTTCACAGACCTTGAGCGCAAAGGACTGCTG; ATTGCGTGTCTGTGTCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAGAAG; TTCGACCACCCTCTGGCCGCTCTCTACTCCACTTCCACCATGGAGCAGCACCACTTCTCC; CAGACTGTGTCCATCCTCCAGTTGGAAGGGCACAATATCTTCTCCACTCTGAGCTCCAGT; GAATATGAGCAGGTGCTTGAGATCATCCGCAAAGCCATCATTGCCACAGACCTTGCTTTA; TACTTTGGAAACAGGAAGCAGTTGGAAGAGATGTACCAGACCGGATCACTAAACCTTAAT; AATCAATCACATAGAGACCGTGTAATTGGTTTGATGATGACTGCCTGTGACCTTTGTTCT; GTGACAAAACTGTGGCCCGTTACAAAATTGACGGCAAATGATATATATGCAGAATTCTGG; GCTGAGGGTGATGAAATGAAGAAATTGGGAATACAGCCTATTCCTATGATGGACAGAGAC; AAGAAGGATGAAGTCCCCCAAGGCCAGCTTGGGTTCTACAATGCCGTGGCCATTCCCTGC; TATACAACCCTTACCCAGATCCTCCCTCCCACGGAGCCTCTTCTGAAAGCATGCAGGGAT; AATCTCAGTCAGTGGGAGAAGGTGATTCGAGGGGAGGAGACTGCAACCTGGATTTCATCC; CCATCCGTGGCTCAGAAGGCAGCTGCATCTGAAGATTGAGCACTGGTCACCCTGACACGC; TGTCCCACCTACAGATCCTCATCTTGCTTCTTTGACATTCTTTTCCTTTTTTTGGGGGGG; GTGGGGGGAACCTGCACCT;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006661 unedited; NGGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGCTC; CCTTGAGCGTCCCCACCAGCAGTAGTCACCGCGGCGGCGGTGGCAGCGGCGGCGGGCGGC; GGCGGCTCTTCCTCTCGCCTGCGCTCCAGGGCTTGCTGCTTCCAGCCCGTGCCGGTCCCC; GGCCGCCGCCGCCGCCCCGGCTCCCCCTTGGCCAGGCCGCCCGCCGCGCGGGCTCCCCCG; GCTTCCCCGGCGCCGGCCCAGGCGGCGGCGGCCAAACTCCGCGGCGTCCCCAGGGCGCCA; GCTTCGCCCTCGCCGCCGCGGCCGCGCTGCTCTTCGGCTCCGACATGGAAGATGGACCTT; CTAATAATGCGAGCTGCTTCCGAAGGCTGACCGAGTGCTTCCTGAGCCCCAGTTTGACAG; ATGAAAAAGTGAAGGCATATCTTTCTCTTCACCCCCAGGTATTAGATGAATTTGTATCTG; AAAGTGTTAGTGCAGAGACAGTAGAGAAATGGCTGAAGAGGAAGAACAACAAATCAGAAG; ATGAATCAGCTCCTAAGGAAGTCAGCAGGTACCAAGATACGAATATGCAGGGAGTTGTAT; ATGAACTAAACAGCTATATAGAACAACGGTTGGACACAGGAGGAGACAACCAGCTACTCC; TCTATGAACTGAGCAGCATCATTAAAATAGCCACAAAAGCCGATGGATTTGCACTGTATT; TCCTTGGAGAGTGCAATAATAGCCTGTGTATATTCACGCCACCTGNGATAAAGGAAGGAA; AACCCCGNCTCATCCCTGCTGGGCCCATCACTCAGGGCACCACCGTCTCTGCTTATGTGG; CCAAGTCCAGGAAACACTGCTAGTAGAAGACTCCTTGGAGATGACGATTNCCAAGAGTAC; TGGACTGGATCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006661 unedited; TAATGGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTATTTTGTTCT; TTATGCAAAGGGTTCAGTAAAACTGCCAANACAACAACAAAAACAATTTTAAAAACTGAC; ATCTTTTGAACTGCTACTTGAAGTTCTGAGATTTATTGTAACATATACCTCATCTCTTCT; CAAAAAGACAGGAAGTCTACTTCGTCTAGTGTTAAATTTATTGATCTCAGCCCTTTAGGT; TGAACTTAAAGAATTATGTTTAGTCTAACTAAATTCATGAAGCTCTGAAATAAGAGTTTG; ACGTTTTGCCATCATTTCTTACTCTGTAACCTCAACGACATTTGTCCTGAGGCTGTGGAC; TACAACTCAAGTTAATTACAAGTAGGTCATACATGAACATTCACCATTCACAATAGTAAT; GTGTAAAAATTCCTATTTATATCCAACAACATCAAAGCAACATTGATGTTAAAGCCCAAG; TACAATACCATAATAGTACCATTTTAAACCCTTATTAGAAAGATACAATGGAAAAGTACC; CCTTCTGGATAATTTTTGTCCATTTCCTTTAAGGCCTCAGCAATATTAGCCTATTAGAAA; AGGCTGGTTTGCAAGTCAAATGGAGTCACTTGGCCAGCTGATTTCTGAACGTGGGGGTGG; TCCTGGTTGCTCAGTGTCTATGATGCCTCACAGAAGAGATTGGCAGGAAGTCCTCTGCTT; GACTTATTTATTTGATGGTACCTTCTTGAAGAGGTTTGCACCCCAGTTACCAGGTGCANG; TTCCCCCCACCCCCCCCAAAAAAAGGAAAAAATGNTCAAAAGCCAGATGAGGATCTCGTA; GTGGGACAGCGTGTCAAGGTGACCAGTGCTCAATCTTCAGATGCAGCTGCCCTCTGAGCC; CCGNATGGGGATGAAAATCCAGTTGCAGGCTCCTCCCTCCAAAAACCTTTTCCCCCTGAC; TGAGATATTACCTGCATGCGTTCAAAAGAGGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_006661
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006661.1, NP_006652.1
• RefSeq Size: 4576 bp
• RefSeq ORF: 2340 bp
• Locus ID: 10846
• UniProt ID: Q9Y233
• Cytogenetics: 6q27
• Domains: GAF, PDEase
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Summary: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2011]; Transcript Variant: This variant (2) has an additional exon in the 5' region, which includes an in-frame AUG start codon, as compared to variant 1. The resulting isoform (2) has an alternate and shorter N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.