GABARAP (NM_007278) Human Untagged Clone

SKU SC115633

GABARAP (untagged)-Human GABA(A) receptor-associated protein (GABARAP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GABARAP
• Synonyms: ATG8A; GABARAP-a; MM46
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115633 sequence for NM_007278 edited (data generated by NextGen Sequencing); ATGAAGTTCGTGTACAAAGAAGAGCATCCGTTCGAGAAGCGCCGCTCTGAGGGCGAGAAG; ATCCGAAAGAAATACCCGGACCGGGTGCCGGTGATAGTAGAAAAGGCTCCCAAAGCTCGG; ATAGGAGACCTGGACAAAAAGAAATACCTGGTGCCTTCTGATCTCACAGTTGGTCAGTTC; TACTTCTTGATCCGGAAGCGAATTCATCTCCGAGCTGAGGATGCCTTGTTTTTCTTTGTC; AACAATGTCATTCCACCCACCAGTGCCACAATGGGTCAGCTGTACCAGGAACACCATGAA; GAAGACTTCTTTCTCTACATTGCCTACAGTGACGAAAGTGTCTACGGTCTGTGA; ; Clone variation with respect to NM_007278.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007278 unedited; GCACGAGGGCCCAGCCCTGTCCAGCCCGGTTCCCGGGAGGATGAAGTTCGTGTACAAAGA; AGAGCATCCGTTCGAGAAGCGCCGCTCTGAGGGCGAGAAGATCCGAAAGAAATACCCGGA; CCGGGTGCCGGTGATAGTAGAAAAGGCTCCCAAAGCTCGGATAGGAGACCTGGACAAAAA; GAAATACCTGGTGCCTTCTGATCTCACAGTTGGTCAGTTCTACTTCTTGATCCGGAAGCG; AATTCATCTCCGAGCTGAGGATGCCTTGTTTTTCTTTGTCAACAATGTCATTCCACCCAC; CAGTGCCACAATGGGTCAGCTGTACCAGGAACACCATGAAGAAGACTTCTTTCTCTACAT; TGCCTACAGTGACGAAAGTGTCTACGGTCTGTGAAGCTGCTGCCCCTGAGCTGGAGGGGG; GTCTCATTCTACAAAGAGAGAGGTGGCCCCCCTTTCTTGACCTCCTCCTCCTTCAAGCTC; AAACACCACCTCCCTTATTCANGACCGGCACTTCTTAATGTTTGTGGCTNTCTCTCCAGC; CTCTCTTANGAGGGGTAATGGTGGGAGTGGCATCTTGTAACTCTCCTTTCTCCTTTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007278 unedited; TGTACGCGGCCGCAATCTANAGTCGAGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGCTGGGGGGTTTCCTTTTTATTG; AGGGGCCTCCTACTTTCCCCCCAAATTTTAAGTCCTTTCCAACTCCCCCCAAAAAAAAAG; GTAGGAAAAGGAAGGGTTTGTTGGGGTTTTAACCCCTTTGGGCAGTCAAAAGGGGAACAA; AACCCAAAACATTCCTTGGTTGGGACACAAACTGACAATAAAAAATTTTACCCCAGGATG; GGAAAGGGGGCCAAAAAAGGGGGAAAAAAGGAAAAAGGAAATTTACAAAATGCCAATTCC; CCCTTTACCCTTTTAAAAAAAGGGTGGAAAAAAACCCACAAACTTTAAAAAGTGCCGGCC; CTAAATAAGGGAGGGGGGGTTTGACCTTAAAGGAGGAGGGGGTCAAAAAAGGGGGGCCCC; CTTTTTTTTTGGAAAAAGAAACCCCCCTCCACCTTAGGGGCAATATTTTCACAACCCGTA; AACCTTTTTTTCATTGTGGGGAATGTAAAAAAAAAAATTTTTTTAATGGGGTCCCTGAAA; CAAATGACCCATTGGGGACTTGGGGGGGGGAATGACATTGTGGAAAAAAAAAACAAGGCC; CCCCCACCCCGGAAAAAAAATTCCCTTCCCGGACAAAAAAATTAAACTGTACCACCTGTG; GAAATCAAAAGGCCCCCATTATTTTTTTTTTTTCCAGATTTCTCTTACCAGAGTTTGGAA; CCTTTTCTTATTTAACCCGGCCCCGCGTCCGGGTATTTTTTTTGGGTTTTTCTCCCTTAA; AGAGGCCCTTTTCAAGAGGATGTCTTTTTTTTCCAAAATTTTTCCTCCCGGGACCGGGTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_007278
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007278.1, NP_009209.1
• RefSeq Size: 924 bp
• RefSeq ORF: 354 bp
• Locus ID: 11337
• UniProt ID: O95166
• Cytogenetics: 17p13.1
• Domains: MAP1_LC3
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy
• Summary: Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]