MRAS (NM_012219) Human Untagged Clone

SKU SC115494

MRAS (untagged)-Human muscle RAS oncogene homolog (MRAS), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: MRAS
• Synonyms: M-RAs; NS11; R-RAS3; RRAS3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_012219 edited; ATGGCAACCAGCGCCGTCCCCAGTGACAACCTCCCCACATACAAGCTGGTGGTGGTGGGG; GATGGGGGTGTGGGCAAAAGTGCCCTCACCATCCAGTTTTTCCAGAAGATCTTTGTGCCT; GACTATGACCCCACCATTGAAGACTCCTACCTGAAACATACGGAGATTGACAATCAATGG; GCCATCTTGGACGTTCTGGACACAGCTGGGCAGGAGGAATTCAGCGCCATGCGGGAGCAA; TACATGCGCACGGGGGATGGCTTCCTCATCGTCTACTCCGTCACTGACAAGGCCAGCTTT; GAGCACGTGGACCGCTTCCACCAGCTTATCCTGCGCGTCAAAGACAGGGAGTCATTCCCG; ATGATCCTCGTGGCCAACAAGGTCGATTTGATGCACTTGAGGAAGATCACCAGGGAGCAA; GGAAAAGAAATGGCGACCAAACACAATACTCCGTACATAGAAACCAGTGCCAAGGACCCA; CCTCTCAATGTCGACAAAGCCTTCCATGACCTCGTTAGAGTAATTAGGCAACAGATTCCG; GAAAAAAGCCAGAAGAAGAAGAAGAAAACCAAATGGCGGGGAGACCGGGCCACAGGCACC; CACAAACTGCAATGTGTGATCTTGTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012219 unedited; GCGAATTCGGCACGAGGGCCGTCAGTCCGGCGGCCGCGGGGCCCGGCGGGCGCGACGCTG; CCTCCTCACCGGCGCAGGCTAGGAGGGGGCGGCCTGAGTGCCGTAGCCGAGCCGGGGCTG; GAGCGCGCGGTCTGACCTACGAGAAACATGGCAACCAGCGCCGTCCCCAGTGACAACCTC; CCCACATACAAGCTGGTGGTGGTGGGGGATGGGGGTGTGNACAAAACATCCCTCACCATC; CACTTTTCTCTCAATACTTTTGTTCCATGTTTTGACCTCCCATTTGCAAACTTGTACGTG; ATACATCAGCTTATTGTCATCTACTGCCTTATCTAGCTCTTACTGCTTACCAATTGCTTC; GAGGAATTAACTTCCTTCCAGCATCAATTACCTTTCTCTTTAAACTGTCTTCCTAATCGT; CCACTACGGTCCTGAAAATTGCAACTTTTAGACATCTTCGACACGTTTCCCAAAGCAAAC; CCTGCACCCGCCAATCTCCTGCAGCTACTACCGGTCTGACACATTTACTCCTCTTTGCCT; TCTTCCTTGTTCTTCATCCACATCACCTGCGCAGCCTTTCTTTTTCATCTCACATCTATC; CCTTTCCACTCTGCTTCACTCCACTCTATGTGCCCAGTCACCCGCATTGTCAACCGTTCG; AAATACCATCCTCGCTGAACGTCCTCTAAACACTCTTATTCCTCCACCATTTCGACACAC; AATCCCCTTATTCAACATCCATATCAACCCCTAATGCCGGCCCCAACCTCTGTCAACTTT; CCTACCCCAACACTCCTACCAGAAAATATCCTCCTCCCAGCCCAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012219 unedited; CTAAGGACCGCGGCCGCATCTANGATCGGTTTTTTTTTTTTTTTTTTTACGTGTTTCTCT; TCTTTATTAATCAGTCGAGAGGAACAAAGACTTAATAAAAAGTTACACAGAAGGTTCAAA; CATCACTGAGATCTTCAGGCATCACTCGTTTCACGACAATGGCCACCTGTTAAGACTATG; AAACAGAAGCTGGGCGCTCACAGGCGCACAATAATTCAAGTATCTTTCATTCCAAGTAAA; CCCGACATGGAATTTCAAACACAATGGGCTGCCAATGGAGGGAACCTGTCTGTGGCTCCA; GATTTGCTGTAACATGTCTGAGGGGCCTTTGGGTTAAAGTTGCCACGTTAGTGGGTCATG; TCTCCTGGAAAAGTGAGACAGGCCCCACGAGGCCAGCCAGGCATTTTCCACAGCAAGTGC; CATGAGAAGGAGGTGGGCAAAGTCCTAAGAGAAACCATCCAGGTGTCCCGAGTGGCAAAG; TGCACAGGTAGTAAGTGGCCATTGTCCCCAGCCGTGGGAGTGGAGAGGATGAGTTGGCTT; ACCTCCGGATAAAAAAGAGCGCACTTGTGAGGAAGTATCAAAGGGCGGTACAGCCCAGAA; CCTAGAAAAGGGGAGAAGAGATGACGAGGGGGCATGTCGGGGAGGGGAAGGAAAGGAGGA; CGCAGAAAATGGCGGGGAGAAACGGAAGCACCACGTAATGGAAAGAGAGCGGGCGTACCA; TCACAGAGGAAATAGGGGAGCGGAACTACACGCAAAAAAAAAGGAGACCAGTCGAACGGG; CAAGATATACACAGAGGGCGAAGGCGCACCGACGGCTGCCGCCAAGATGCAAAAGAGGCA; AAGAGAGAAACCACCGGAGGCCTATGTTAAGAGAGAAAGGATACAGCGAGTTCCAGACAA; GAGAGGGTAAGGATAAGAGGGCCAAACATAAGGAAAGGACACAACAGACGGCATAGACAG; AAAAGGCAATATAAGCCGACCAGAATGAGAGAAGTCAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_012219
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012219.2, NP_036351.2
• RefSeq Size: 3926 bp
• RefSeq ORF: 627 bp
• Locus ID: 22808
• UniProt ID: O14807
• Cytogenetics: 3q22.3
• Domains: RAB, RAN, ras, RAS, RHO
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction
• Summary: This gene encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same isoform (1).