GADD45B (NM_015675) Human Untagged Clone

SKU SC114608

GADD45B (untagged)-Human growth arrest and DNA-damage-inducible, beta (GADD45B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GADD45B
• Synonyms: GADD45BETA; MYD118
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114608 sequence for NM_015675 edited (data generated by NextGen Sequencing); ATGACGCTGGAAGAGCTCGTGGCGTGCGACAACGCGGCGCAGAAGATGCAGACGGTGACC; GCCGCGGTGGAGGAGCTTTTGGTGGCCGCTCAGCGCCAGGATCGCCTCACAGTGGGGGTG; TACGAGTCGGCCAAGTTGATGAATGTGGACCCAGACAGCGTGGTCCTCTGCCTCTTGGCC; ATTGACGAGGAGGAGGAGGATGACATCGCCCTGCAAATCCACTTCACGCTCATCCAGTCC; TTCTGCTGTGACAACGACATCAACATCGTGCGGGTGTCGGGCATGCAGCGCCTGGCGCAG; CTCCTGGGAGAGCCGGCCGAGACCCAGGGCACCACCGAGGCCCGAGACCTGCATTGTCTC; CTGGTCACGAACCCTCACACGGACGCCTGGAAGAGCCACGGCTTGGTGGAGGTGGCCAGC; TACTGCGAAGAAAGCCGGGGCAACAACCAGTGGGTCCCCTACATCTCTCTTCAGGAACGC; TGA; ; Clone variation with respect to NM_015675.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015675 unedited; NGGGCACCATTTGTATACGACTCCTATAGGCGGCCGCNGAATTCGCACGAGGGTCGGACT; ACCGTTGGTTTCCGCAACTTCCTGGATTATCCTCGCCAAGGACTTTGCAATATATTTTTC; CGCCTTTTCTGGAAGGATTTCGCTGCTTCCCGAAGGTCTTGGACGAGCGCTCTAGCTCTG; TGGGAAGGTTTTGGGCTCTCTGGCTCGGATTTTGCAATTTCTCCCTGGGGACTGCCGTGG; AGCCGCATCCACTGTGGATTATAATTGCAACATGACGCTGGAAGAGCTCGTGGCGTGCGA; CAACGCGGCGCAGAAGATGCAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCGC; TCAGCGCCAGGATCGCCTCACAGTGGGGGTGTACGAGTCGGCCAAGTTGATGAATGTGGA; CCCAGACAGCGTGGTCCTCTGCCTCTTGGCCATTGACGAGGAGGAGGAGGATGACATCGC; CCTGCAAATCCACTTCACGCTCATCCAGTCCTTCTGCTGTGACAACGACATCAACATCGT; GCGGGTGTCGGGCATGCAGCGCCTGGCGCAGCTCCTGNGAGAGCCGGCCGAGACCCAGGG; CACCACCGAGGCCCGAGACCTGCATTGTCTCCTGGTCACGAACCCTCACACGGACGCCTG; GAAGAGCCACGGCTTGGTGGAGGTGGCCAGCTACTGCGAAGAAAGCCCGGGCAAACACCA; GTGNGTCCCCTACATCTCTCTTCAGGAACGCTNGAGCCCTTNCCAGCAGCAGAATCTGTT; GAGTTGCTGCCCACAACCAAAATACATAATATTTTTGGACCCCCTTCCCCCCAGCCAACC; CCCCCAAAACACCCACCCCACGAGACATCGGNGGCANAGTCNTGGAGACTGAANAGAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015675 unedited; GCAATCTAGTATCGAGTNNTTTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTGAGGCTTTAAAAATTTATTACAAGGCCCTCCTAGTAGAAATAAAAAT; ATAGATTTCTATGCTTCCCATCTCGCTCTCAGGGGGTCGAATAACAAGTGCAAGGAACAA; AATAAATTGGCTCTATAATTCGCAAACTGGGAGTTCATGGGTACAGAACAACTTCAGCCC; CAGCTCCCAAGTCCCAAAGTGGGGTCTTGTCGAGGGGGCAAACAAGGGCCAACCAAGTTC; AACCAAGTCTTTTGTATGCAAACCCCAGCTCCAGTCTTAAGGAGGGGGGGGCTTGCAGTC; AATCTCACTCCACCCCCGAGTGGACAGTCTGGACCCTCCCTGATGGGGAAAGCGGNATCG; TGCCCCCCCACTCCGGGTTTTGGTCCATCCCAAGGCCTCAACTTTGGGGGTCCTGTCTCC; TGCTGGCCTGGGTCCCCCCTTTTCCATCGGATCGGCGGCCGCCAACTCCTGGATCTCCGC; CCTGGGGGCGGGCGCTCACTCCCCTTTTCCTCCTCCTTTTCCTCTTAAGACTCCAACGAC; TTTGGCCCCGATAGTCTCCTGGGTTGGGTTGTTTTGGGGGAGTTGTGCCTGGGGGGGAGA; GGGCCTTTCCCCCCCCCCCCCCCCTCTCCCTCCCCCCCCTCTCTCTTCATTTCCCTCCTC; CTCCTCTCCACGTCTTTCCCCCTCCTCTATCTCCCTCCTTCCCGCCCCGCACCTCTTCCT; TCTTCTCCTCCTCGTTTCCCTCACTCTCTCGCCCCTTGTTGTGGCCTACCTCGTGCCCTC; TTTGTTTCTTCTCCTTTCCCCTCCACCCTATCCCTCACCTTCTTCTATTCCTCTCTATCC; TCCACACCCGCCTCCCTCCCTTTCTACACTGTTACTCCTGTATCCTCGTATCCATCCCCC; CCAACACTCTGCTCTCTCTGTTCTTTCTATTCTCCCCTCTCCTACACCGCCTTCCAATCT; C;
• Restriction Sites: NotI-NotI
• ACCN: NM_015675
• Insert Size: 1420 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015675.1, NP_056490.1
• RefSeq Size: 1121 bp
• RefSeq ORF: 486 bp
• Locus ID: 4616
• UniProt ID: O75293
• Cytogenetics: 19p13.3
• Domains: Ribosomal_L7Ae
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, MAPK signaling pathway, p53 signaling pathway
• Summary: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond to environmental stresses by mediating activation of the p38/JNK pathway. This activation is mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. The function of these genes or their protein products is involved in the regulation of growth and apoptosis. These genes are regulated by different mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting cell growth. [provided by RefSeq, Jul 2008]