Shwachman Bodian Diamond syndrome (SBDS) (NM_016038) Human Untagged Clone

SKU SC114508

SBDS (untagged)-Human Shwachman-Bodian-Diamond syndrome (SBDS)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Shwachman Bodian Diamond syndrome
• Synonyms: CGI-97; SDO1; SDS; SWDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114508 sequence for NM_016038 edited (data generated by NextGen Sequencing); ATGTCGATCTTCACCCCCACCAACCAGATCCGCCTAACCAATGTGGCCGTGGTACGGATG; AAGCGTGCCGGGAAGCGCTTCGAAATCGCCTGCTACAAAAACAAGGTCGTCGGCTGGCGG; AGCGGCGTGGAAAAAGACCTCGATGAAGTTCTGCAGACCCACTCAGTGTTTGTAAATGTT; TCTAAAGGTCAGGTTGCCAAAAAGGAAGATCTCATCAGTGCGTTTGGAACAGATGACCAA; ACTGAAATCTGTAAGCAGATTTTGACTAAAGGAGAAGTTCAAGTATCAGATAAAGAAAGA; CACACACAACTGGAGCAGATGTTTAGGGACATTGCAACTATTGTGGCAGACAAATGTGTG; AATCCTGAAACAAAGAGACCATACACCGTGATCCTTATTGAGAGAGCCATGAAGGACATC; CACTATTCGGTGAAAACCAACAAGAGTACAAAACAGCAGGCTTTGGAAGTGATAAAGCAG; TTAAAAGAGAAAATGAAGATAGAACGTGCTCACATGAGGCTTCGGTTCATCCTTCCAGTC; AATGAAGGCAAGAAGCTGAAAGAAAAGCTCAAGCCACTGATCAAGGTCATAGAAAGTGAA; GATTATGGCCAACAGTTAGAAATCGTATGTCTGATTGACCCGGGCTGCTTCCGAGAAATT; GATGAGCTAATAAAAAAGGAAACTAAAGGCAAAGGTTCTTTGGAAGTACTCAATCTGAAA; GATGTAGAAGAAGGAGATGAGAAATTTGAATGA; ; Clone variation with respect to NM_016038.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016038 unedited; GTGCACCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCAGAC; ACACTGTGACGGCTGCCTGAAGCTAGTGAGTCGCGGCGCCGCGCACTGGTGGTTGGGTCA; GTGCCGCGCGCCGATCGGTCGTTACCGCGAGGCGCTGGTGGCCTTCAGGCTGGACGGCGC; GGGTCAGCCCTGGTTCGCCGGCTTCTGGGTCTTTGAACAGCCGCGATGTCGATCTTCACC; CCCACCAACCAGATCCGCCTAACCAATGTGGCCGTGGTACGGATGAAGCGTGCCGGGAAG; CGCTTCGAAATCGCCTGCTACAAAAACAAGGTCGTCGGCTGGCGGAGCGGCGTGGAAAAA; GACCTCGATGAAGTTCTGCAGACCCACTCAGTGTTTGTAAATGTTTCTAAAGGTCAGGTT; GCCAAAAAGGAAGATCTCATCAGTGCGTTTGGAACAGATGACCAAACTGAAATCTGTAAG; CAGATTTTGACTAAAGGAGAAGTTCAAGTATCAGATAAAGAAAGACACACACAACTGGAG; CAGATGTTTAGGGACATTGCAACTATTGTGGCAGACAAATGTGTGAATCCTGAAACAAAG; AGACCATACACCGTGATCCTTATTGAGAGAGCCATGAAGGACATCCACTATTCGGTGAAA; ACCAACAAGAGTACAAAACAGCAGGCTTTGGAAGTGATAAAGCAGTTAAAAGAGAAAATG; AAGATAGAACGTGCTCACATGAGGCCTTCGGTTCATCCTTCCAGTCAATGAAAGCAAGAA; GCTGAAAGAAAGCTCAAGCACTGATCAAGTCATAGAAGTGAAGATTATGCCCACAGTTAG; AAACGTATGTCTGATTGACCCGGGCTGCTTTCGAGAAATTGATGAGCTCATTAAAAAGGA; ACTAAGGCCAAGGTTCCTTTGGAGTACTCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016038 unedited; GTACCGCGGGCCGCAATCTANAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTAATAA; ACATAAGAGTGGTTTTATTGATTACATACAATTTTAGCTATATTAATATATATTATAAAC; TTTAAGAATTAGAAATAAGTGACTTTTATTTTTTAACCAAGAATAATCTAAGTTATGGCA; GCATGTTCAATGAAAGGTAAGTCCGGCACAATTTTTCTATATCTGTTTCTCAGATAATCA; GGAACATCATCCAAGCTTTACATTACGATACCATAATGACCCTCAGAACACAAGTTCCAT; TAAGTAGAAATGAAGCATCATATGTTTTCTTTTTTAGGAAAGACCCCCCCTTTTGTTGTA; TAGACATACCCCTAATAATCTTACTCTACTGTACAAATAACTTTTCACCCACAAGAGCTG; CCTCAAGTAACTTTCATTTTGGAAAGCTATCAAGGCATGAGACAGAGTAGCAAAATGCCA; CTCTGGACTTTGCATCTTGGAGTTTCAATTTTGCTTTAGGATTTAGACCCCAGTTTATAA; TTAAAACCAAATCATTCCCACATTATTATACTTATGTAGGAAAGCCTTGCTGTGTCCACT; GTTAACACAAAGATAGAAAATGTCAAATAGTTTAAGCAAGTATTTGGCAGACCACAGACA; TGAAACAGTGCCGTCGGAAACGGAACACTTTAGTGTTTAGAGGTGAACAGATGACCGGTG; TCATTCAAATTCTAATCTCCTTCTTCTACTCTTTCAGATTGAGTACTTCCAAGCACCTTT; GCCTTTAGTTCCTTTTTAATACCTATCAATTCCTCGGAGCAGCCCGGNTCATCAAAATAC; CATTTCTCACTGTTGGCCATAATCTCCACTTTCTATGACCTTGATCAGGGGCTGGAGCCC; TTCTTTAAGTTTCTGCCTCCATGCCTGGAGGATAACCCAACCTCTGTGAGCACGTTTATC; TATTTCTCTTAACTGCTTAACCTCCAAGCCCCCGTTGGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_016038
• Insert Size: 1570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016038.2, NP_057122.2
• RefSeq Size: 1605 bp
• RefSeq ORF: 753 bp
• Locus ID: 51119
• UniProt ID: Q9Y3A5
• Cytogenetics: 7q11.21
• Domains: UPF0023
• Summary: This gene encodes a highly conserved protein that plays an essential role in ribosome biogenesis. The encoded protein interacts with elongation factor-like GTPase 1 to disassociate eukaryotic initiation factor 6 from the late cytoplasmic pre-60S ribosomal subunit allowing assembly of the 80S subunit. Mutations within this gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome. This gene has a closely linked pseudogene that is distally located. [provided by RefSeq, Jan 2017]