N acetyl transferase 5 (NAA20) (NM_016100) Human Untagged Clone

SKU SC114456

NAA20 (untagged)-Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: N acetyl transferase 5
• Synonyms: dJ1002M8.1; NAT3; NAT3P; NAT5; NAT5P
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114456 sequence for NM_016100 edited (data generated by NextGen Sequencing); ATGACCACGCTACGGGCCTTTACCTGCGACGACCTGTTCCGCTTCAACAACATTAACTTG; GATCCACTTACAGAAACTTATGGGATTCCTTTCTACCTACAATACCTCGCCCACTGGCCA; GAGTATTTCATTGTTGCAGAGGCACCTGGTGGAGAATTAATGGGTTATATTATGGGTAAA; GCAGAAGGCTCAGTAGCTAGGGAAGAATGGCACGGGCACGTCACAGCTCTGTCTGTTGCC; CCAGAATTTCGACGCCTTGGTTTGGCTGCTAAACTTATGGAGTTACTAGAGGAGATTTCA; GAAAGAAAGGGTGGATTTTTTGTGGATCTCTTTGTAAGAGTATCTAACCAAGTTGCAGTT; AACATGTACAAGCAGTTGGGCTACAGTGTATATAGGACGGTCATAGAGTACTATTCGGCC; AGCAACGGGGAGCCTGATGAGGACGCTTATGATATGAGGAAAGCACTTTCCAGGGATACT; GAGAAGAAATCCATCATACCATTACCTCATCCTGTGAGGCCTGAAGACATTGAATAA; ; Clone variation with respect to NM_016100.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016100 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCTCGTGCCGATTCGGCA; CGAGGGTCTTCGGAAGCGGCGGCGGCGCGATGACCACGCTACGGGCCTTTACCTGCGACG; ACCTGTTCCGCTTCAACAACATTAACTTGGATCCACTTACAGAAACTTATGGGATTCCTT; TCTACCTACAATACCTCGCCCACTGGCCAGAGTATTTCATTGTTGCAGAGGCACCTGGTG; GAGAATTAATGGGTTATATTATGGGTAAAGCAGAAGGCTCAGTAGCTAGGGAAGAATGGC; ACGGGCACGTCACAGCTCTGTCTGTTGCCCCAGAATTTCGACGCCTTGGTTTGGCTGCTA; AACTTATGGAGTTACTAGAGGAGATTTCAGAAAGAAAGGGTGGATTTTTTGTGGATCTCT; TTGTAAGAGTATCTAACCAAGTTGCAGTTAACATGTACAAGCAGTTGGGCTACAGTGTAT; ATAGGACGGTCATAGAGTACTATTCGGCCAGCAACGGNGAGCCTGATGAGGACGCTTATG; ATATGAGGAAAGCACTTTCCAGGGATACTGAGAAGAAATCCATCATACCATTACCTCATC; CTGTGAGGCCTGAAGACATTGAATAACCCTGGGCAGTGGTTCTTAGGCAGATACTCTAGA; TGCTTTATGGACAATATTATTTTCATTGGATGATTCTGGAGCTCTATTANGAGAAAAAGT; ATCATTTTAGGTCTTAAAGACTTCAGAAAATACAGGTTATCAATNTATTNTAAATCTCAT; TGTTTCCAGTTAGCAATATCATACCTATTNAAGCTGTTCATTGGTACANAATNNNCATCA; AAAGCCAGCTAGTCAGAAGGAAACATACACTCTCATGGTNATAGAATCACTGTATGTATG; CTAGGGAAAAGACTGCTCCATCTCCCTCTCAGTCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016100 unedited; GCAATCTAGTATCGAGTTCTTTTTTTTTTTTTTTTTTTTTTAGAGTCAATAATGTATTAT; ACATCTTAAATTTATACATTTCATATATGCTTTTAAAGCTTCAATTAACAGTTCAATACA; AAATTTAAAAACAAATATATGCAGCAGTGGTTCTCAGGCACAGAACTGAGGAGGAGACTG; GAGCAAGTCTTTTCCCTAGCATACATACAGTGAATACTATGAACCATGAGAGTGGTATGT; TTCCTTCTGACCTAGCTGCCTTTTTGATTGAATTTTGTTACAATGAACAGCTTTAATAGG; TATGATATTGCTAACTGGAAACAATGAGATTTAAAATAAATTGATAACCTGTATTTTCTT; GAAGTCTTTAAGACCTAAAATGATTACTTTTCTCCTAATAGAGCTCCAGAATCATCCAAT; GAAAATAATATTGTCCATAAAGCATCTAGAGTATCTGCCTAAGAACCACTGCCCAGGGTT; ATTCAATGTCTTCAGGCCTCACAGGATGAGGTAATGGTATGATGGATTTCTTCTCAGTAT; CCCTGGAAAGAGCTTTCCTCATATCATAAGCGTCCTCATCAGGCTCCCCGTTGCTGGCCG; AATAGTACTCTATGACCGTCCTATATACACTGTAGCCCAACTGCTNGTACATGTTAACTG; CAACTTGGTTAGATACTCTTTTACAAGAGATCCACAAAAATCCACCCCTTTCTTTCTGAA; ATCTCCTCTAGTAACTCCATAAGTTTAGCAGCCCAACCAAGGCGTTGAAAATCCTGGGCA; ACAGACAGAGCTGTGACGTGCCCGTGCATTTTTTCCTACTACTGAGCCTTCTGCTTAACC; ATAATATACCCTTAATTCTCACCAGGTGCTCTGCACATGAAATACTCTGCCAGGGGCCAG; TATTGTAGAAAAGGATCCCTAAGTTCGTAATGGATCATTAATGTGTGACCGACACGTCTC; CAAGTAAGCCCTACGGGTATCCCCCCCCCGCTTCAAAACTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_016100
• Insert Size: 1070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016100.2, NP_057184.1
• RefSeq Size: 1360 bp
• RefSeq ORF: 537 bp
• Locus ID: 51126
• UniProt ID: A6NHA3
• Cytogenetics: 20p11.23
• Domains: Acetyltransf
• Protein Pathways: Glycerophospholipid metabolism, Limonene and pinene degradation, Phenylalanine metabolism, Tyrosine metabolism
• Summary: NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009]; Transcript Variant: This variant (1) encodes the longest isoform (a).