DNAJB12 (NM_017626) Human Untagged Clone

SKU SC114056

DNAJB12 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNAJB12
• Synonyms: DJ10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017626, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTCATCACTCCGCGCCCGGCTGCCCGCGACGCGCCGGCGGGTGGCGCAGCCCTTCGCTCGCCCGGCCT; CCCCCTCCCTGGTTCCGCGTTCTGGTTCCGCCATGGAATCCAACAAGGATGAAGCTGAGCGCTGTATCAG; CATCGCCCTCAAGGCCATCCAGAGCAACCAGCCCGACCGGGCGCTCCGCTTCCTGGAGAAGGCACAGCGG; CTGTATCCGACGCCGCGAGTTCGCGCCCTGATTGAGTCCCTCAACCAGAAACCACAGACTGCCGGTGACC; AACCCCCACCCACAGACACAACCCATGCCACCCACAGGAAAGCAGGTGGGACCGATGCCCCCTCGGCCAA; CGGTGAAGCTGGAGGAGAGAGCACCAAAGGCTACACTGCAGAACAGGTTGCAGCTGTGAAAAGGGTCAAG; CAATGTAAAGATTACTATGAGATCCTGGGGGTGAGCAGAGGGGCCTCGGATGAGGACCTGAAGAAGGCCT; ACCGCAGACTGGCCCTCAAATTCCACCCAGACAAGAACCACGCACCTGGTGCCACTGAAGCCTTCAAAGC; CATTGGCACAGCATATGCGGTACTCAGCAACCCGGAGAAGAGGAAGCAGTATGACCAGTTCGGCGATGAC; AAGAGCCAGGCGGCCCGGCACGGCCATGGGCATGGGGATTTCCACCGTGGCTTTGAGGCCGACATCTCCC; CTGAAGACCTCTTCAACATGTTCTTTGGCGGCGGCTTCCCTTCTAGTAACGTCCACGTCTACAGCAACGG; CCGCATGCGCTATACCTACCAGCAAAGGCAGGACCGCAGGGACAACCAGGGTGATGGCGGGCTAGGGGTG; TTTGTGCAGCTGATGCCTATCCTCATCCTGATTCTCGTGTCAGCTCTCAGCCAGCTCATGGTCTCCAGTC; CACCCTACAGTCTGAGTCCAAGACCGTCCGTGGGCCACATCCACAGGCGAGTCACTGACCACCTGGGTGT; CGTCTACTATGTGGGAGACACTTTCTCCGAAGAGTACACAGGCTCCAGCCTCAAAACAGTCGAGCGGAAT; GTGGAAGATGATTATATCGCCAACCTCCGGAACAACTGCTGGAAGGAGAAGCAGCAGAAGGAAGGCTTGC; TGTACCGGGCACGCTACTTTGGCGACACAGATATGTACCACAGAGCACAGAAGATGGGCACCCCCAGCTG; CAGCCGACTGTCAGAGGTGCAGGCCTCCCTGCATGGATAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017626 unedited; GTGTTCANATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAAGGGCTGGT; TCCGCCATGGNAATCCAACAAGNATGAAGCTGAGCGCTGTATCAGCATCGCCCTCAAGGC; CATCCAGAGCAACCAGCCCGACCGGGCGCTCCGCTTCCTGGAGAAGGCACAGCGGCTGTA; TCCGACGCCGCGAGTTCGCGCCCTGATTGAGTCCCTCAACCAGAAACCACAGACTGCCGG; TGACCAACCCCCACCCACAGACACAACCCATGCCACCCACAGGAAAGCAGGTGGGACCGA; TGCCCCCTCGGCCAACGGTGAAGCTGGAGGAGAGAGCACCAAAGGCTACACTGCAGAACA; GGTTGCAGCTGTGAAAAGGGTCAAGCAATGTAAAGATTACTATGAGATCCTGGGGGTGAG; CAGAGGGGCCTCGGATGAGGACCTGAAGAAGGCCTACCGCAGACTGGCCCTCAAATTCCA; CCCAGACAAGAACCACGCACCTGGTGCCACTGAAGCCTTCAAAGCCATTGGCACAGCATA; TGCGGTACTCAGCAACCCGGAGAAGAGGAAGCAGTATGACCAGTTCGGCGATGACAAGAG; CCAGGCGGCCCGGCACGGCCATGGGCATGGGGATTTCCACCGTGGCTTTGAGGCCGACAT; CTCCCCTGAAGACCTCTTCAACATGTTCTTTGGCGGCGGCTTCCCTTCTAGTAACGTCCA; CGTCTACAGCAACGGCCGCATGCGCTATACCTACCAGCAAAGGCAGGACCGCAGGGACAA; CCAGGGTGATGGCGGGCTAGGGGTGTTTGTGCAGCTGATGCCTATCCTCATCCTGATTCT; CGTGTCAGCTCTCAGCCAGCTCATGGTCTCCAGTNCACCCTACAGTCTGAGTCCAGACCG; TCCGTGGCCACANTCACAGCGAGTACTGACACCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017626 unedited; TGTACGCGGCCGCTTCTAAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA; CAATTTGAGAATTCTAATTCAAAAAAACAGCTTCCAAAGGATCCTTTAGGATCTATGTTC; TTATTGCACATGGCTCAACCCGTCTAGGGACATCTACCAAGACCATATGGTCGGGGCGTG; GGGGCAAATCACCGGGGCCACAGTGGGTAGGCACTCTGGCCACATCAGTTCTTATAATCT; CTCTGGGCTGAAGTGGCTGCTTCATTGCAGGGTTGGACCTTGTTTAAAAACCAAATTTGA; CATTTCCTTACCGAACTTCCTTTCCCAGCAAGAATCCTATTTGTTGGGGGACTTTTAAAA; AAAGAGCCGAGAGAAACTCTGGGAGGGTGGTTATCACCTCCTGGCTGGCAGCTTGGGGAA; GTAAGGCTTTGGAAGACGGCGGGCGCAATGACCCACCCACCGCCACAGGAAAACATGGTC; ATTCCATAAGGCCCAGGAGAAACTGGGGGAATAAATAAAACCTCCCTCCTCCCACTGGCG; GCAAGTGCTGTTTTAAGCCAAAATCCCTCATTTTCAATGGGAGGGTAAGAAAACTATTCT; GGTTCAGGTGTATCCCTTTGTCCAGGTACCCAGAGGAGGCCTTGGCGTCACTTAACAGCG; AGTGAATACAGAGGCAGCCACGGGCACTCGCCTTTCTTCAAGAGTCAAGGAGGTTGCCCT; GTGGTCCCGGCACCACTGACTGACTTGGGAAGAAAAACTTTTTGAAATCCTTTGTGGCAT; GACTTAGAAAAAACCATGGCAATGAAGGAGCTGTGCCCTGTGGGCCCTTTAAGGAAGCCC; CCTTGGGGACTTAAGGGGGGGAAAAGGTTAGTAAATCCTTGGCCCTCTGGAGGCCTTCTT; TTTTGGGCAGAGGCTGAAGCTCCTGGCCCAAAAAAAACCCACCCCTTTTGAAGGGAAAGA; GTCTCCCAACAGTCTTGGGGCCCTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017626
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017626.3, NP_060096.2
• RefSeq Size: 3215 bp
• RefSeq ORF: 1128 bp
• Locus ID: 54788
• UniProt ID: Q9NXW2
• Cytogenetics: 10q22.1
• Domains: DnaJ
• Protein Families: Transmembrane
• Summary: DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 4 encode the same protein. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.