C20orf20 (MRGBP) (NM_018270) Human Untagged Clone

SKU
SC113588
MRGBP (untagged)-Human chromosome 20 open reading frame 20 (C20orf20)
$300.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol C20orf20
Synonyms C20orf20; Eaf7; MRG15BP; URCC4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_018270, the custom clone sequence may differ by one or more nucleotides


ATGGGAGAGGCCGAGGTGGGCGGCGGGGGCGCCGCAGGCGACAAGGGCCCGGGGGAGGCGGCCACCAGCC
CGGCGGAGGAGACAGTGGTGTGGAGCCCCGAGGTGGAGGTGTGCCTCTTCCACGCCATGCTGGGCCACAA
GCCCGTCGGTGTGAACCGACACTTCCACATGATTTGTATTCGGGACAAGTTCAGCCAGAACATCGGGCGG
CAGGTCCCATCCAAGGTCATCTGGGACCATCTGAGCACCATGTACGACATGCAGGCGCTGCATGAGTCTG
AGATTCTTCCATTCCCGAATCCAGAGAGGAACTTCGTCCTTCCAGAAGAGATCATTCAGGAGGTCCGAGA
AGGAAAAGTGATGATAGAAGAGGAGATGAAAGAGGAGATGAAGGAAGACGTGGACCCCCACAATGGGGCT
GACGATGTTTTTTCATCTTCAGGGAGTTTGGGGAAAGCATCAGAAAAATCCAGCAAAGACAAAGAGAAGA
ACTCCTCAGACTTGGGGTGCAAAGAAGGCGCAGACAAGCGGAAGCGCAGCCGGGTCACCGACAAAGTCCT
GACCGCAAACAGCAACCCTTCCAGTCCCAGTGCTGCCAAGCGGCGCCGCACGTAG


5' Read Nucleotide Sequence
>OriGene 5' read for NM_018270 unedited
TTCGAATTTTGTAATACGACTTACTATTAGGGCGGCCGCGAATTCGCACCAGGGCCGCGC
CTGCTCCCGCCGGGGGCTCCTTGCTCGGCCGGGCCGCGGCCATGGGAGAGGCCGAGGTGG
GCGGCGGGGGCGCCGCAGGCGACAAGGGCCCGGGGGAGGCGGCCACCAGCCCGGCGGAGG
AGACAGTGGTGTGGAGCCCCGAGGTGGAGGTGTGCCTCTTCCACGCCATGCTGGGCCACA
AGCCCGTCGGTGTGAACCGACACTTCCACATGATTTGTATTCGGGACAAGTTCAGCCAGA
ACATCGGGCGGCAGGTCCCATCCAAGGTCATCTGGGACCATCTGAGCACCATGTACGACA
TGCAGGCGCTGCATGAGTCTGAGATTCTTCCATTCCCGAATCCAGAGAGGAACTTCGTCC
TTCCAGAAGAGATCATTCAGGAGGTCCGAGAAGGAAAAGTGATGATAGAAGAGGAGATGA
AAGAGGAGATGAAGGAAGACGTGGACCCCCACAATGGGGCTGACGATGTTTTTTCATCTT
CAGGGAGTTTGGGGAAAGCATCAGAAAAATCCAGCAAAGACAAAGAGAAGAACTCCTCAG
ACTTGGGGTGCAAAGAAGGCGCAGACAAGCGGAAGCGCAGCCGGGTCACCGACAAAGTCC
TGACCGCAAACAGCAACCCTTCCAGTCCCAGTGCTGCCAAGCGGCGCCGCACGTAGACCC
TCAGCCCTGGTGGCGGCAGAGAAGCGGGCGAGGCACTGTGGTCGCTGAGGGGGTTGGCTG
GGTCTGAGTGCCACCCNCCAGCCACAGTGATACCATCCCAGTGCCATGAGCCCACACTGG
CCCGNCCCCTCAGCTCTCAGTGAAACGTGCCGTCAGCGGGGAAACGTGTGTGTCAGTTGG
ACATGTGGGACCCTGATGGACCTGAAGACCAGGATCGTCCACTCAGATATGAGGGCTCTG
AGCTAGTCTGTCTCTCTGGACACTT
Restriction Sites NotI-NotI
ACCN NM_018270
Insert Size 1650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018270.3, NP_060740.1
RefSeq Size 1685 bp
RefSeq ORF 615 bp
Locus ID 55257
UniProt ID Q9NV56
Cytogenetics 20q13.33
Protein Families Transcription Factors
Summary Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.[UniProtKB/Swiss-Prot Function]
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Citations

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