CLN8 (NM_018941) Human Untagged Clone

SKU SC113338

CLN8 (untagged)-Human ceroid-lipofuscinosis, neuronal 8 (epilepsy, progressive with mental retardation) (CLN8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CLN8
• Synonyms: C8orf61; EPMR; TLCD6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113338 sequence for NM_018941 edited (data generated by NextGen Sequencing); ATGAATCCTGCGAGCGATGGGGGCACATCAGAGAGCATTTTTGACCTGGACTATGCATCC; TGGGGGATCCGCTCCACGCTGATGGTCGCTGGCTTTGTCTTCTACTTGGGCGTCTTTGTG; GTCTGCCACCAGCTGTCCTCTTCCCTGAATGCCACTTACCGTTCTTTGGTGGCCAGAGAG; AAGGTCTTCTGGGACCTGGCGGCCACGCGTGCAGTCTTTGGTGTTCAGAGCACAGCCGCA; GGCCTGTGGGCTCTGCTGGGGGACCCTGTGCTGCATGCCGACAAGGCGCGTGGCCAGCAG; AACTGGTGCTGGTTTCACATCACGACAGCAACGGGATTCTTTTGCTTTGAAAATGTTGCA; GTCCACCTGTCCAACTTGATCTTCCGGACATTTGACTTGTTTCTGGTTATCCACCATCTC; TTTGCCTTTCTTGGGTTTCTTGGCTGCTTGGTCAATCTCCAAGCTGGCCACTATCTAGCT; ATGACCACGTTGCTCCTGGAGATGAGCACGCCCTTTACCTGCGTTTCCTGGATGCTCTTA; AAGGCGGGCTGGTCCGAGTCTCTGTTTTGGAAGCTCAACCAGTGGCTGATGATTCACATG; TTTCACTGCCGCATGGTTCTAACCTACCACATGTGGTGGGTGTGTTTCTGGCACTGGGAC; GGCCTGGTCAGCAGCCTGTATCTGCCTCATTTGACACTGTTCCTTGTCGGACTGGCTCTG; CTTACGCTAATCATTAATCCATATTGGACCCATAAGAAGACTCAGCAGCTTCTCAATCCG; GTGGACTGGAACTTCGCACAGCCAGAAGCCAAGAGCAGGCCAGAAGGCAACGGGCAGCTG; CTGCGGAAGAAGAGGCCATAG; ; Clone variation with respect to NM_018941.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018941 unedited; AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCAGTGACTGCCGGGAGT; CCTGCANGGGCNTTNNNCCGCCAAGCGCAGGGAGCCCGGCTGAGTGGCAGCCCAGATTGA; AGATGGATACGTGACAATCCCAGGGACCGCTGCACTGACTTCATTTCCTTAGACAAGACA; CAGTGTAGGGCCCGGCCCGTGTTGGCCCCAGGACTCCTTTGGAATATAGCTGTGGACAAT; GAATCCTGCGAGCGATGGGGGCACATCAGAGAGCATTTTTGACCTGGACTATGCATCCTG; GGGGATCCGCTCCACGCTGATGGTCGCTGGCTTTGTCTTCTACTTGGGCGTCTTTGTGGT; CTGCCACCAGCTGTCCTCTTCCCTGAATGCCACTTACCGTTCTTTGGTGGCCAGAGAGAA; GGTCTTCTGGGACCTGGCGGCCACGCGTGCAGTCTTTGGTGTTCAGAGCACAGCCGCAGG; CCTGTGGGCTCTGCTGGGGACCCTGTGCTGCATGCCGACANGGCGCGTGGCCAGCAGAAC; TGGTGCTGGTTTACATCACGACAGCAACGGGATTTCTTTTGCTTTGAAATGTTGCAGTCC; ACCTGTCCAACTTGATCTTCCGGACATTGACTTGGTTCTGGTTATCCACCATCTCTTTGC; CTTTCTTGGGGTTCCTGCTGGCTGGTCAATCTCCAGCTTGCCACTAATTAGTTATGACAA; CGGGCTTCTGAGATGAGCACGCCCTTTACTTGCGTTTCTGGAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_018941
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018941.2, NP_061764.2
• RefSeq Size: 4817 bp
• RefSeq ORF: 861 bp
• Locus ID: 2055
• UniProt ID: Q9UBY8
• Cytogenetics: 8p23.3
• Domains: TLC
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a transmembrane protein belonging to a family of proteins containing TLC domains, which are postulated to function in lipid synthesis, transport, or sensing. The protein localizes to the endoplasmic reticulum (ER), and may recycle between the ER and ER-Golgi intermediate compartment. Mutations in this gene are associated with a disorder characterized by progressive epilepsy with cognitive disabilities (EPMR), which is a subtype of neuronal ceroid lipofuscinoses (NCL). Patients with mutations in this gene have altered levels of sphingolipid and phospholipids in the brain. [provided by RefSeq, Jul 2017]