NCKX2 (SLC24A2) (NM_020344) Human Untagged Clone

SKU SC113130

SLC24A2 (untagged)-Human solute carrier family 24 (sodium/potassium/calcium exchanger), member 2 (SLC24A2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NCKX2
• Synonyms: NCKX2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020344, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGATCTGCAACAAAGCACCACCATCACTTCCCTAGAGAAATGGTGTTTGGATGAGTCACTGTCTGGCT; GCAGAAGACATTATAGTGTCAAGAAAAAACTGAAGTTAATTCGAGTCTTAGGCCTTTTCATGGGTCTGGT; AGCCATTAGCACTGTCTCATTTTCAATCAGTGCCTTTTCTGAGACAGATACACAGAGCACAGGAGAGGCC; AGTGTTGTAAGTGGCCCTAGGGTAGCACAGGGTTACCATCAGAGAACTCTCTTAGATTTAAATGACAAGA; TTCTGGATTATACTCCACAGCCACCTCTTTCTAAGGAAGGCGAGTCTGAGAATAGTACAGATCACGCCCA; AGGAGACTACCCGAAAGACATCTTTTCCCTTGAGGAGAGAAGAAAAGGTGCGATCATTCTGCATGTCATT; GGAATGATCTACATGTTCATAGCCTTAGCCATTGTCTGTGATGAGTTCTTTGTTCCTTCTTTGACTGTCA; TCACTGAAAAACTGGGCATCTCTGATGATGTGGCTGGAGCCACCTTCATGGCTGCAGGAGGGTCAGCCCC; AGAACTTTTCACATCTCTCATAGGGGTATTTATCGCTCACAGCAACGTTGGCATAGGCACAATTGTAGGT; TCAGCAGTATTCAACATCCTCTTTGTTATTGGCATGTGTGCTCTGTTTTCTAGAGAAATCTTAAACCTGA; CATGGTGGCCGCTCTTTCGAGATGTGTCTTTCTACATTGTTGACTTGATCATGCTGATCATATTTTTCCT; GGATAATGTCATCATGTGGTGGGAAAGCTTGCTTCTCTTAACAGCTTATTTTTGCTATGTGGTTTTCATG; AAATTCAACGTCCAAGTAGAAAAATGGGTGAAGCAAATGATAAACCGCAATAAGGTCGTCAAGGTGACAG; CACCAGAAGCCCAAGCAAAGCCATCTGCAGCCAGGGACAAGGATGAACCAACTCTACCGGCTAAGCCGCG; TCTCCAGCGAGGTGGAAGCTCTGCCTCCCTCCACAACAGTCTCATGAGGAATAGCATCTTCCAACTCATG; ATACACACCCTTGACCCACTCGCCGAAGAACTTGGATCATATGGAAAACTAAAATATTATGACACAATGA; CTGAAGAAGGGAGGTTCAGAGAAAAGGCTTCAATTCTCCACAAGATCGCCAAGAAGAAATGTCATGTGGA; TGAGAACGAGAGGCAGAATGGGGCTGCCAACCACGTGGAAAAAATTGAGCTTCCAAACAGCACCAGCACA; GATGTTGAAATGACACCATCCAGTGATGCTTCAGAACCTGTACAAAATGGAAATCTCTCCCACAACATTG; AAGGTGCAGAAGCCCAGACCGCTGATGAGGAGGAGGACCAGCCTCTCAGCCTTGCCTGGCCTTCTGAAAC; CCGCAAGCAAGTCACGTTTCTGATTGTTTTCCCCATAGTGTTTCCTCTCTGGATTACGTTACCTGACGTT; CGCAAACCTTCATCGAGGAAGTTTTTTCCCATCACGTTCTTTGGCTCCATTACCTGGATTGCAGTATTCT; CTTACTTGATGGTCTGGTGGGCGCACCAGGTTGGAGAGACAATTGGCATCAGTGAAGAGATTATGGGCCT; GACCATCTTGGCTGCTGGGACCTCCATCCCTGATCTTATCACCAGTGTCATAGTGGCCCGGAAGGGGTTA; GGGGACATGGCTGTGTCCAGCTCTGTTGGAAGCAACATTTTTGACATCACTGTAGGGCTCCCACTGCCCT; GGCTCCTGTACACCGTCATTCACAGATTCCAGCCAGTGGCTGTCAGCAGCAATGGCCTTTTCTGTGCCAT; CGTCCTTCTCTTCATCATGCTGCTCTTCGTCATCCTCTCTATCGCCCTCTGCAAGTGGCGAATGAACAAA; ATCCTGGGCTTCATCATGTTTGGCCTCTACTTTGTGTTCCTGGTGGTGAGCGTTCTCCTAGAAGACAGAA; TTCTTACATGCCCCGTCTCCATCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020344 unedited; GGGGGGGNNNNGGGGGNNNNNNNNNNNNTNNNNNNNNNTTTTTTACACCCGCCCGTTGNC; NCAAAGGGCGGTAGGCGTGTACGGTGGGNGGTCTATATAAGCAGAGCTCATTTAGGTGAC; ACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCATA; AGATGACCCCAAAGTTGACAGCACTTTCAGGCTTTGTGAATTAGGCGCGTCACCGACCTG; TACGATTTCTCCTCTCTGCCCTTGCTCGCCTGCAAATCCTGACCCCCGTCCACACTGCGG; AGAAATCTCCTAAGAGACGGCTGCCAGCGTCGCCTCTGCGCCCAGGAGGCGTGGAGCAAA; GGACTCGGGAGCTGAGCGCGCTGCGGCCCCGCTCCCAGCGCACAGCGCACAGCCCAGAGG; GAGGGGCCGGCGGGGGCCGGGCAGCGCCAGACTCGGAGCGCCGGGCTCACAGCGGCTCAG; TCTCCTCCCGAACCCAGCCCGGCTGGCGAGAGCGGCAGCCAGTGCGAGCCAGAGCCCGGG; CCTCAGAGCGGGTAGGCGGAGGGCCGGCGGACTGGAGCGGCGGGCCGCGCTCGGGGGCCC; CGCCGTGTGCGCGTCCTCCCATCTTATCCTGCATAAATGATGAAAGTTTCCTGTGAAGCA; TAACAATCCCTGATTGTGAAAGTACCCCACAAGCATCGNTAACTATAAAGTAAGGAAAAG; TATGAAAGAACCATCTGGTTGAGTCTAAAGTTGGGAAGATCACCATATCCACCAGAAGAC; CCAGNATGGATCTGNCACANAGCACCACCATCACTTCCCTAGAAAAATGGTGTTTGGATG; AGTCACTGTCTGGCTGCAGAAGACTTATAGTGTCAGGAAAAACGGAAGNTAATTCGAGTC; TTAGCCTTTTCATGGGNTCTGGTAGCCATTACCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020344 unedited; CGTACATTATGNACGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGGGCCACCCATTTTTTTTAAATTGGGGAAT; ACCATGGTGGCCCTTGAATTGTTGATATCTTTTATACCCCGCCCCCAAAAAACAAAAAAA; GTTTCTGCCGCCCAAATTTTTGCTTTGGATTTTCTGGGGGGGTTTCTTTGCCCCTTTTTC; AACATTAGGGATATGGCTGACCCATTTCCTTAAGGCTTTAAATTGGTTTTCTTAAGGGGG; GAAAAAATGGGGCCCAATGGGGATATTTGGAAAAATATGGGCCGGGAACAAACAACCTTT; TTAAATCCGGAAAAAATGTTCCCTTTGGGTTGGGGGCAAGAATTTCCTTTTCCTTGACTT; TGGCAAACTTGATGCCCCCCGCTTTAAAATCCCGAATGGGAAAGGGCCAAAAGGGGATGA; TGTAAACCAAATTATATGGGGCCCAATAAATCCGGGATAAAAATTTTTTTATATATAGGG; GTATATTTAAAAATGGGAATACCCCGGCCTGCCGGAAACCAGTTAAAAATGACCCGGTTT; GGAAACCCCGGGGGGGAAAGTTCTTATAATTATGCCCATGATTTGAATGGGTTAAAGACC; CCTTGGTGAACTCTGGACCCATCTCTCTGAATTTACCCTGGCTCAAACATAACCCCTTTC; CGACCCCAAAATTAAGGGAAAGCCAGGGGCCGGCCGGGGAATCCCTTGGGGCCCCATCCA; ATGAAGGAACAAGGCTTGTGTTAGTCAAGCCCAATGCACATGACTGATTTGGGAACGGGG; CTTGGAAAATTTTGTCCTCCCAAAAGCACCTTCACCTGAAAAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_020344
• Insert Size: 6000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020344.1, NP_065077.1
• RefSeq Size: 10857 bp
• RefSeq ORF: 10857 bp
• Locus ID: 25769
• UniProt ID: Q9UI40
• Cytogenetics: 9p22.1-p21.3
• Domains: Na_Ca_Ex
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a member of the calcium/cation antiporter superfamily of transport proteins. The encoded protein belongs to the SLC24 branch of exchangers, which can mediate the extrusion of one Ca2+ ion and one K+ ion in exchange for four Na+ ions. This family member is a retinal cone/brain exchanger that can mediate a light-induced decrease in free Ca2+ concentration. This protein may also play a neuroprotective role during ischemic brain injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.