TIGAR (NM_020375) Human Untagged Clone

SKU SC113108

C12orf5 (untagged)-Human chromosome 12 open reading frame 5 (C12orf5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TIGAR
• Synonyms: C12orf5; FR2BP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113108 sequence for NM_020375 edited (data generated by NextGen Sequencing); ATGGCTCGCTTCGCTCTGACTGTTGTCCGGCATGGAGAAACAAGATTTAACAAGGAGAAA; ATAATCCAAGGACAAGGAGTAGATGAACCTCTTTCAGAAACTGGATTTAAACAAGCAGCA; GCTGCTGGTATATTTCTGAATAATGTGAAGTTTACTCATGCTTTCTCCAGTGATCTCATG; AGGACAAAGCAGACCATGCATGGAATTTTGGAGAGAAGCAAATTTTGCAAAGATATGACG; GTAAAGTATGACTCAAGACTTCGGGAAAGGAAATACGGGGTTGTAGAAGGCAAAGCGCTA; AGTGAGCTGAGGGCCATGGCCAAAGCAGCCAGGGAAGAGTGCCCTGTGTTTACACCGCCC; GGAGGAGAGACGCTGGACCAGGTGAAAATGCGTGGAATAGACTTTTTTGAATTTCTTTGT; CAACTAATCCTGAAAGAAGCGGATCAAAAAGAACAGTTTTCCCAAGGATCTCCAAGCAAC; TGTCTGGAAACTTCTTTGGCAGAGATATTTCCTTTAGGAAAAAATCACAGCTCTAAAGTT; AATTCAGACAGCGGTATTCCAGGATTAGCAGCCAGTGTCTTAGTTGTGAGTCACGGTGCT; TACATGAGAAGTCTGTTTGATTATTTTCTGACTGACCTTAAGTGTTCCTTACCAGCCACT; CTGAGCAGATCTGAACTTATGTCAGTCACTCCCAATACAGGGATGAGTCTCTTTATCATA; AACTTTGAGGAAGGAAGAGAAGTTAAACCAACGGTTCAGTGTATTTGTATGAACCTACAG; GATCATCTAAATGGACTGACTGAAACTCGCTAA; ; Clone variation with respect to NM_020375.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020375 unedited; NTGTTCAGATTTTGNTATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGGCC; CGCAGTGCAGGGGCAGCGCGGCGCGGGGCCACCGACGGGACGCGGCTCCGGGAACATGGC; TCGCTTCGCTCTGACTGTTGTCCGGCATGGAGAAACAAGATTTAACAAGGAGAAAATAAT; CCAAGGACAAGGAGTAGATGAACCTCTTTCAGAAACTGGATTTAAACAAGCAGCAGCTGC; TGGTATATTTCTGAATAATGTGAAGTTTACTCATGCTTTCTCCAGTGATCTCATGAGGAC; AAAGCAGACCATGCATGGAATTTTGGAGAGAAGCAAATTTTGCAAAGATATGACGGTAAA; GTATGACTCAAGACTTCGGGAAAGGAAATACGGGGTTGTAGAAGGCAAAGCGCTAAGTGA; GCTGAGGGCCATGGCCAAAGCAGCCAGGGAAGAGTGCCCTGTGTTTACACCGCCCGGAGG; AGAGACGCTGGACCAGGTGAAAATGCGTGGAATAGACTTTTTTGAATTTCTTTGTCAACT; AATCCTGAAAGAAGCGGATCAAAAAGAACAGCTTTCCCAAGGATCTCCAAGCAACTGTCT; GGAAACTTCTTTGGCAGAGATATTTCCTTTACGAAAAAATCACAGCTCTAAAGTTAATTC; AGACAGCGGTATTCCAGGATTAGCAGCCAGTGTCTTAGTTGTGAGTCACGGTGCTCACAT; GAGAAGCCTGTTTGATTATTTTCTGACTGACCTTAAGTGTTCCCTACCAGCCACTCTGAG; CAGATCTGAACTTATGTCAGTCACCTCCCATACAGGGATGAGTCCTTTTATTATAACCTT; GAAGAAGGAAGAGAATTAAACCACCGGCCCATGGAATTGAATGAACCAACGGATCTCTAA; TGGACTGACG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020375 unedited; CGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTAAACTTATAAGGATT; TTTTATTTAAAGGATTTAAAAATATAACACAGTCAATATAAACATGTACTGGGAATTATA; AACCATTCTTTCTTCTAAGCACTGGATGAGATACTAAAAACATACAGTATCTTACCAATA; GCCATTAAAATAGGCTAAAATGAAAAAGAAACCGTTGTAACAAGGTTACTAATCCCCCAA; CTTTCAATGCTGAGTTCCTTCATCATCCATGTGCAATCCAGAGATGACATCTAGCAGGGT; GGTAAAATTATTCTGGAAAATGCCAACTGTACTTGGACAAAATAAGTTAATTCTATATGG; TTGTCCATTAAAGTTTTATGTGGCTATGGTTCCACTGGAGCTAAAAATTGGCTTTTAACT; GTTTCCAAATCAGAACTAGCAGAGGAGAGAAGTAAATAAAGCCAATGGCACTCCCTTCAG; AGGCTCAAAATGGTTAGATTTTGATGCAGATTTAACCTTAGCGAGTTTCAGTCAGTCCAT; TTAGATGATCCTGTAGGTTCATACAAATACACTGAACCGTTGGTTTAACTTCTCTTCCTT; CCTCAAAGTTTATGATAAAGAGACTCATCCCTGTATTGGGAGTGACTGACATAAGTTCAG; ATCTGCTCAGAGTGGCTGGTAAGGAACACTTAAGGTCAGTCAGAAAATAATCAAACAGAC; TTCTCATGTAAGCACCGTGACTCACAACTAAGACACTGGCTGCTAATCCTGGAATACCGC; TGTCTGAATTAACTTAGAGCTGTGATTTTTTCCTAAAGGAATATCTCTGCCAAAGAAGTT; TCAGACAGTTGCTTGGAGATCCTTGGGAAAACTGTCTTTTTGATCCGCTTCTTCAGGGAT; AGTTGACAAAGAATCAAAAAGNCTATTCCAGCTTTTCACTGGCCAGCGTTTTCCTCGGGC; GTGTAAACCAGGCACTCTTCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_020375
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020375.1, NP_065108.1
• RefSeq Size: 1361 bp
• RefSeq ORF: 813 bp
• Locus ID: 57103
• UniProt ID: Q9NQ88
• Cytogenetics: 12p13.32
• Domains: PGAM
• Summary: This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]