HEPC (HAMP) (NM_021175) Human Untagged Clone

CAT#: SC112995

HAMP (untagged)-Human hepcidin antimicrobial peptide (HAMP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HEPC
• Synonyms: HEPC; HFE2B; LEAP1; PLTR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_021175 edited
  ATGGCACTGAGCTCCCAGATCTGGGCCGCTTGCCTCCTGCTCCTCCTCCTCCTCGCCAGC
  CTGACCAGTGGCTCTGTTTTCCCACAACAGACGGGACAACTTGCAGAGCTGCAACCCCAG
  GACAGAGCTGGAGCCAGGGCCAGCTGGATGCCCATGTTCCAGAGGCGAAGGAGGCGAGAC
  ACCCACTTCCCCATCTGCATTTTCTGCTGCGGCTGCTGTCATCGATCAAAGTGTGGGATG
  TGCTGCAAGACGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021175 unedited
  NGGGTCAGATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACCAAAAGACCCAG
  CAGTGGGACAGCCAGACAGACGGCACGATGGCACTGAGCTCCCAGATCTGGGCCGCTTGC
  CTCCTGCTCCTCCTCCTCCTCGCCAGCCTGACCAGTGGCTCTGTTTTCCCACAACAGACG
  GGACAACTTGCAGAGCTGCAACCCCAGGACAGAGCTGGAGCCAGGGCCAGCTGGATGCCC
  ATGTTCCAGAGGCGAAGGAGGCGAGACACCCACTTCCCCATCTGCATTTTCTGCTGCGGC
  TGCTGTCATCGATCAAAGTGTGGGATGTGCTGCAAGACGTAGAACCTACCTGCCCTGCCC
  CCGTCCCCTCCCTTCCTTATTTATTCCTGCTGCCCCAGAACATAGGTCTTGGAATAAAAT
  GGCTGGTTCTTTTGTTTTCCAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCG
  CGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGC
  CTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAA
  GTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGNGGTG
  GTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGNGTCTATTGGGAA
  CCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAA
  GCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGNCATGCATNGACCAGCTC
  AGCTAATTTTTGGTTTTTTGGTAGAGACGGGGNTTCACCATATTGGGCCAGCTGGTCTCC
  AACTCTAATCTCAGTGATCTACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021175
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021175.2, NP_066998.1
• RefSeq Size: 430 bp
• RefSeq ORF: 255 bp
• Locus ID: 57817
• UniProt ID: P81172
• Cytogenetics: 19q13.12
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Oct 2014]