C20orf77 (RPRD1B) (NM_021215) Human Untagged Clone

SKU SC112934

RPRD1B (untagged)-Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: C20orf77
• Synonyms: C20orf77; CREPT; dJ1057B20.2; K-H; Kub5-Hera; NET60
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112934 sequence for NM_021215 edited (data generated by NextGen Sequencing); ATGTCCTCCTTCTCTGAGTCGGCGCTGGAGAAGAAGCTCTCGGAGCTGAGCAACTCTCAG; CAGAGCGTGCAGACCCTGTCCCTTTGGCTCATCCACCACCGCAAGCACGCGGGACCCATC; GTCTCCGTGTGGCACCGCGAGCTCCGCAAAGCCAAATCAAATAGAAAGCTTACTTTTCTG; TATTTAGCGAATGATGTCATCCAAAACAGTAAAAGGAAAGGACCTGAATTCACTAGAGAA; TTTGAATCTGTCCTTGTGGATGCTTTTTCTCATGTTGCCAGAGAGGCAGATGAAGGCTGT; AAAAAACCTTTAGAAAGATTGCTGAACATCTGGCAAGAACGAAGTGTGTATGGCGGCGAG; TTCATACAGCAGCTGAAGCTGTCTATGGAGGACTCCAAGAGCCCTCCCCCCAAAGCAACA; GAAGAGAAGAAATCTCTGAAACGAACTTTTCAGCAAATTCAGGAGGAGGAGGATGACGAC; TACCCTGGCAGCTACTCTCCTCAGGATCCTTCTGCAGGACCCCTCTTGACTGAGGAACTA; ATCAAAGCTTTGCAGGATCTGGAAAATGCCGCATCAGGGGATGCTACTGTCCGACAGAAA; ATTGCTTCTCTGCCCCAGGAAGTGCAAGATGTTTCTCTATTGGAAAAAATAACAGACAAA; GAGGCAGCTGAACGTCTTTCAAAAACAGTAGATGAAGCATGTCTGTTACTAGCAGAATAT; AACGGGCGCCTGGCAGCAGAACTGGAGGACCGTCGCCAGCTGGCTCGGATGTTGGTGGAG; TATACCCAGAATCAGAAAGATGTTTTGTCGGAGAAGGAGAAAAAACTAGAGGAATACAAA; CAGAAGCTTGCACGAGTAACCCAGGTCCGCAAGGAACTGAAATCCCATATTCAGAGCTTG; CCAGACCTCTCACTGCTGCCCAACGTCACAGGGGGCTTAGCCCCCCTGCCCTCTGCTGGG; GACCTGTTTTCAACTGACTAG; ; Clone variation with respect to NM_021215.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021215 unedited; GGGTGTTCAAATATTTGTATACGACTTCACTATAGGGCGGCCGCGAATCGGCACGAGGCG; GGGAACTACTCTCCTTGCCTCGCTCTGTCTCCTTCGAAGTGCTCTGCGCGAGGTTCAGAG; CGGCCGCCGCCTCCAAAGGGACGGTTTTCTAGAGCTCCGACGCCTCTCGGTGCCCCTCTG; CTCCGGCCCTTGCCCTTTGACCTCGCTCTCGCGGCAGGGTGAGAGGTCGGGTGGCCATCT; TGTGGCGGCGGCGCGGGCGGCTGTTACTGCGGAGACCCATCCCCTCCCCCTTCTCGCACC; CCTGGCAGTCTGTCAGTCGGTAAAAAGTCCCGCAGCCTGTCAGGTGAGGCCCCGGCCTCG; TGCCGTCGCTCTTCCCGCCGCACTGGGCGGCCCAGGCCGCTCCCTGCCGGGCCTCACTGC; CGCCACCATGTCCTCCTTCTCTGAGTCGGCGCTGGAGAAGAAGCTCTCGGAGCTGAGCAA; CTCTCAGCAGAGCGTGCAGACCCTGTCCCTTTGGCTCATCCACCACCGCAAGCACGCGGG; ACCCATCGTCTCCGTGTGGCACCGCGAGCTCCGCAAAGCCAAATCAAATAGAAAGCTTAC; TTTTCTGTATTTAGCGAATGATGTCATCCAAAACAGTAAAAGGAAAGGACCTGAATTCAC; TAGAGAATTTGAATCTGTCCTTGTGGATGCTTTTTCTCATGTTGCCAGAGAGGCAGATGA; AAGCTGTAAAAAACCTTTAGAAAGATTGCTGAACATCTGGCAAGAACGAAGTGTGTATGG; CCGGCGAGTTCATACAGCAGCTGAAGCTGTCTATTGCAGGACTCCAAGAGCCCTCCCCCC; ANAGCACCAGAAAGAGAAGAAATCTCTGAAACGAACTTTTAGCCAATCAGGAGGGAGGAG; GATGACGACTACCCTGCCAGCTACTCTCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021215 unedited; CGCGGCACGCTATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTAAACAACACCAT; CTTTATTCAAAAATATATATCTATGAAACATTTCAAAATATACTGCTGCCGCCAACGACA; GCCTATACTTCTAAATAGTCCTAAATTAATATACAAATTTAAGCTTTGAAAATATTCTTT; AAGAAAAACAAACAAACAAAAACAAGGGAAGATTGAAGACTTTCTGAGGAATATGACCTA; AAGGAAAGGTCCTTTGAGGCCTACAGATGCCCTAAGAAAGAAAAAACACAAAACCAAAAA; CCACCCACCCCACCCACCAAGTCACCTCATACATACAAGGAGGAAAGTTCATTTAAATTA; AACAAATGAAGTAAGCACACTTTGGGAGGATGTGGACAGCAGATTTAAGATTTAAAAAAC; GAAAGCATTTCATCAAAACAAAAAACTAACTTCCTCACAGGAAGTCCACTATTAACAGGT; ATTAAGAGGCCAACACATTCATTTACATAATTGCTACAGTTTCATTTCTTAACTTTTGCT; GAATAATTATGCACCATTTGCTGGCAAAAAACTCAGTGGGATAAAACAGAGCAGTGGGCT; AAATCCATCCGGCTTTATTTTCATCTAATTGATCAGAGCAAATATCTACAATCCTTTTTT; GGCTGGATTGGGGCGGCTAATTCGCACTTCCTTGGAGAAGCCTTAAAGGTCCAAAAGGGC; CTGCTCCAAACACCCTCCTCTAAAAGCCCCACCCCCGGCGGGCCGGGGATAGGTGCCGCC; TCACCCTCCATTTCATTCAACACCTCCTCGCCTTTTTAAGTATCCACAGTACCAGAGACG; TACACCTCTCGCCCAATCTACCCCGATGGCGACATACATCGCTACTTCACTATCCACCAC; GAGCACTNTACTTCCATCCATATCTTTAAACACTTATTCTGATCTTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_021215
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021215.2, NP_067038.1
• RefSeq Size: 4219 bp
• RefSeq ORF: 981 bp
• Locus ID: 58490
• UniProt ID: Q9NQG5
• Cytogenetics: 20q11.23
• Domains: DUF618, RPR
• Summary: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.[UniProtKB/Swiss-Prot Function]