C20orf77 (RPRD1B) (NM_021215) Human Tagged ORF Clone

SKU RC207481

RPRD1B (Myc-DDK-tagged)-Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: C20orf77
• Synonyms: C20orf77; CREPT; dJ1057B20.2; K-H; Kub5-Hera; NET60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207481 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCCTCCTTCTCTGAGTCGGCGCTGGAGAAGAAGCTCTCGGAGCTGAGCAACTCTCAGCACAGCGTGC; AGACCCTGTCCCTTTGGCTCATCCACCACCGCAAGCACGCGGGACCCATCGTCTCCGTGTGGCACCGCGA; GCTCCGCAAAGCCAAATCAAATAGAAAGCTTACTTTTCTGTATTTAGCGAATGATGTCATCCAAAACAGT; AAAAGGAAAGGACCTGAATTCACTAGAGAATTTGAATCTGTCCTTGTGGATGCTTTTTCTCATGTTGCCA; GAGAGGCAGATGAAGGCTGTAAAAAACCTTTAGAAAGATTGCTGAACATCTGGCAAGAACGAAGTGTGTA; TGGCGGCGAGTTCATACAGCAGCTGAAGCTGTCTATGGAGGACTCCAAGAGCCCTCCCCCCAAAGCAACA; GAAGAGAAGAAATCTCTGAAACGAACTTTTCAGCAAATTCAGGAGGAGGAGGATGACGACTACCCTGGCA; GCTACTCTCCTCAGGATCCTTCTGCAGGACCCCTCTTGACTGAGGAACTAATCAAAGCTTTGCAGGATCT; GGAAAATGCCGCATCAGGGGATGCTACTGTCCGACAGAAAATTGCTTCTCTGCCCCAGGAAGTGCAAGAT; GTTTCTCTATTGGAAAAAATAACAGACAAAGAGGCAGCTGAACGTCTTTCAAAAACAGTAGATGAAGCAT; GTCTGTTACTAGCAGAATATAACGGGCGCCTGGCAGCAGAACTGGAGGACCGTCGCCAGCTGGCTCGGAT; GTTGGTGGAGTATACCCAGAATCAGAAAGATGTTTTGTCGGAGAAGGAGAAAAAACTAGAGGAATACAAA; CAGAAGCTTGCACGAGTAACCCAGGTCCGCAAGGAACTGAAATCCCATATTCAGAGCTTGCCAGACCTCT; CACTGCTGCCCAACGTCACAGGGGGCTTAGCCCCCCTGCCCTCTGCTGGGGACCTGTTTTCAACTGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207481 protein sequence; Red=Cloning site Green=Tags(s); ; MSSFSESALEKKLSELSNSQHSVQTLSLWLIHHRKHAGPIVSVWHRELRKAKSNRKLTFLYLANDVIQNS; KRKGPEFTREFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKAT; EEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELIKALQDLENAASGDATVRQKIASLPQEVQD; VSLLEKITDKEAAERLSKTVDEACLLLAEYNGRLAAELEDRRQLARMLVEYTQNQKDVLSEKEKKLEEYK; QKLARVTQVRKELKSHIQSLPDLSLLPNVTGGLAPLPSAGDLFSTD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021215
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021215.4
• RefSeq Size: 3895 bp
• RefSeq ORF: 981 bp
• Locus ID: 58490
• UniProt ID: Q9NQG5
• Cytogenetics: 20q11.23
• Domains: DUF618, RPR
• MW: 36.9 kDa
• Summary: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.[UniProtKB/Swiss-Prot Function]