HDAC3 (NM_003883) Human Untagged Clone

CAT#: SC112704

HDAC3 (untagged)-Human histone deacetylase 3 (HDAC3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HDAC3
• Synonyms: HD3; KDAC3; RPD3; RPD3-2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112704 sequence for NM_003883 edited (data generated by NextGen Sequencing)
  ATGGCCAAGACCGTGGCCTATTTCTACGACCCCGACGTGGGCAACTTCCACTACGGAGCT
  GGACACCCTATGAAGCCCCATCGCCTGGCATTGACCCATAGCCTGGTCCTGCATTACGGT
  CTCTATAAGAAGATGATCGTCTTCAAGCCATACCAGGCCTCCCAGCATGACATGTGCCGC
  TTCCACTCCGAGGACTACATTGACTTCCTGCAGAGAGTCAGCCCCACCAATATGCAAGGC
  TTCACCAAGAGTCTTAATGCCTTCAACGTAGGCGATGACTGCCCAGTGTTTCCCGGGCTC
  TTTGAGTTCTGCTCGCGTTACACAGGCGCATCTCTGCAAGGAGCAACCCAGCTGAACAAC
  AAGATCTGTGATATTGCCATTAACTGGGCTGGTGGTCTGCACCATGCCAAGAAGTTTGAG
  GCCTCTGGCTTCTGCTATGTCAACGACATTGTGATTGGCATCCTGGAGCTGCTCAAGTAC
  CACCCTCGGGTGCTCTACATTGACATTGACATCCACCATGGTGACGGGGTTCAAGAAGCT
  TTCTACCTCACTGACCGGGTCATGACGGTGTCCTTCCACAAATACGGAAATTACTTCTTC
  CCTGGCACAGGTGACATGTATGAAGTCGGGGCAGAGAGTGGCCGCTACTACTGTCTGAAC
  GTGCCCCTGCGGGATGGCATTGATGACCAGAGTTACAAGCACCTTTTCCAGCCGGTTATC
  AACCAGGTAGTGGACTTCTACCAACCCACGTGCATTGTGCTCCAGTGTGGAGCTGACTCT
  CTGGGCTGTGATCGATTGGGCTGCTTTAACCTCAGCATCCGAGGGCATGGGGAATGCGTT
  GAATATGTCAAGAGCTTCAATATCCCTCTACTCGTGCTGGGTGGTGGTGGTTATACTGTC
  CGAAATGTTGCCCGCTGCTGGACATATGAGACATCGCTGCTGGTAGAAGAGGCCATTAGT
  GAGGAGCTTCCCTATAGTGAATACTTCGAGTACTTTGCCCCAGACTTCACACTTCATCCA
  GATGTCAGCACCCGCATCGAGAATCAGAACTCACGCCAGTATCTGGACCAGATCCGCCAG
  ACAATCTTTGAAAACCTGAAGATGCTGAACCATGCACCTAGTGTCCAGATTCATGACGTG
  CCTGCAGACCTCCTGACCTATGACAGGACTGATGAGGCTGATGCAGAGGAGAGGGGTCCT
  GAGGAGAACTATAGCAGGCCAGAGGCACCCAATGAGTTCTATGATGGAGACCATGACAAT
  GACAAGGAAAGCGATGTGGAGATTTAA
  
  Clone variation with respect to NM_003883.3
  165 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003883 unedited
  NNNCCTGTCANAAATTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACCAGGCCGC
  GGGCGGCGGGCGGCGGAGGTGCGGGGCCTGCTCCCGCCGGCACCATGGCCAAGACCGTGG
  CCTATTTCTACGACCCCGACGTGGGCAACTTCCACTACGGAGCTGGACACCCTATGAAGC
  CCCATCGCCTGGCATTGACCCATAGCCTGGTCCTGCATTACGGTCTCTATAAGAAGATGA
  TCGTCTTCAAGCCATACCAGGCCTCCCAGCATGACATGTGCCGCTTCCACTCCGAGGACT
  ACATTGACTTCCTGCAGAGAGTCAGCCCCACCAATATGCAAGGCTTCACCAAGAGTCTTA
  ATGCCTTCAACGTAGGCGATGACTGCCCAGTGTTTCCCGGGCTCTTTGAGTTCTGCTCGC
  GTTACACAGGCGCATCTCTGCAAGGAGCAACCCAGCTGAACAACAAGATCTGTGATATTG
  CCATTAACTGGGCTGGTGGTCTGCACCATGCCAAGAAGTTTGAGGCCTCTGGCTTCTGCT
  ATGTCAACGACATTGTGATTGGCATCCTGGAGCTGCTCAAGTACCACCCTCGGGTGCTCT
  ACATTGACATTGACATCCACCATGGTGACGGNGTTCAAGAAGCTTTCTACCTCACTGACC
  GNGTCATGACGGTGTCCTTCCACAAATACGGAAATTACTTCTTCCCTGGCACAGGTGACA
  TGTATGAAGTCGGNGCAGAGAGTGGCCGCTACTACTGTCTGAACGTGCCCCTGCGGNATG
  GCATTGATGACCAGAGNTACAAGCACCTTTTCCAGCCGGNTATCAACCAGNTAGTGGACT
  TCTACCAACCCACGTGCATTGTGCTTCAGTGTGGAGCTGACTCTCTGGCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003883 unedited
  CCAAAAGTGAGTTTCAGACATTTTCATATCCTCCCCACACTTGAAAACATACTTCCCATC
  ATCTGGGATTCAGGTGTTAGGGAGCCAGAGCCCCTTCCAAATCTCTCTCTCTTCATCTTC
  CCTGGAAGCAAGGGGAGGAAGAAGTCAGAACCCAGAGGATGAGGTANNTANAGCAATCTC
  AGTCCTTGTCTACCCGTTCATCCCTCAAAAATCTCTGGGTTCGAGGGAAGCAGGGAAGAA
  ATAAGGGGCAAGGGGGGCTAGGGACTGGCCTCCAGGGCCCACTGCCAATAATGTCCCAGA
  TAGCTATCCTTGTCTGTATCTCATCCCTAATAGGTACCATTGTCAGGCCTTGGGAGAGAG
  AGGAAAAGCAGGTAGATGGTTCGAGAACCAAATGTGGTCTCCAGACTCTTTCCCAGAGTC
  AGCAAAAGCCCTGGGGTGAACCCCAGGACTCTAGGAGCCACTCCTTTTCCCTCCAGCCCA
  ACCAAGAGGTGAAAAGAAATTCCTTGGGACACAGCATCCCAAGCCACTCTTAAATCTCCA
  CATCGCTTTCCTTGTCATTGTCATGGTCTCCATCATAGAACTCATTGGGTGCCTCTGGCC
  TGCTATAGTTCTCCTCAGGACCCCTCTCCTCTGCATCAGCCTCATCAGTCCTGTCATAGG
  TCAGGAGGTCTGCAGGCACGTCATGAATCTGGACACTAGGTGCATGGTTCAGCATCTTCA
  GGTTTTCAAAGATTGTCTGGCGGATCTGGTCCAGATACTGGCGTGAGTTCTGATTCTCGA
  TGCCGGTGCTGACATCTGGATGAAGTGTGAAGTCTGGGGCAAAGTACTCGAAGTATTCCC
  TAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003883
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003883.2, NP_003874.2
• RefSeq Size: 1955 bp
• RefSeq ORF: 1287 bp
• Locus ID: 8841
• UniProt ID: O15379
• Cytogenetics: 5q31.3
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.