HDAC3 (NM_003883) Human Untagged Clone

SKU SC112704

HDAC3 (untagged)-Human histone deacetylase 3 (HDAC3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HDAC3
• Synonyms: HD3; KDAC3; RPD3; RPD3-2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112704 sequence for NM_003883 edited (data generated by NextGen Sequencing); ATGGCCAAGACCGTGGCCTATTTCTACGACCCCGACGTGGGCAACTTCCACTACGGAGCT; GGACACCCTATGAAGCCCCATCGCCTGGCATTGACCCATAGCCTGGTCCTGCATTACGGT; CTCTATAAGAAGATGATCGTCTTCAAGCCATACCAGGCCTCCCAGCATGACATGTGCCGC; TTCCACTCCGAGGACTACATTGACTTCCTGCAGAGAGTCAGCCCCACCAATATGCAAGGC; TTCACCAAGAGTCTTAATGCCTTCAACGTAGGCGATGACTGCCCAGTGTTTCCCGGGCTC; TTTGAGTTCTGCTCGCGTTACACAGGCGCATCTCTGCAAGGAGCAACCCAGCTGAACAAC; AAGATCTGTGATATTGCCATTAACTGGGCTGGTGGTCTGCACCATGCCAAGAAGTTTGAG; GCCTCTGGCTTCTGCTATGTCAACGACATTGTGATTGGCATCCTGGAGCTGCTCAAGTAC; CACCCTCGGGTGCTCTACATTGACATTGACATCCACCATGGTGACGGGGTTCAAGAAGCT; TTCTACCTCACTGACCGGGTCATGACGGTGTCCTTCCACAAATACGGAAATTACTTCTTC; CCTGGCACAGGTGACATGTATGAAGTCGGGGCAGAGAGTGGCCGCTACTACTGTCTGAAC; GTGCCCCTGCGGGATGGCATTGATGACCAGAGTTACAAGCACCTTTTCCAGCCGGTTATC; AACCAGGTAGTGGACTTCTACCAACCCACGTGCATTGTGCTCCAGTGTGGAGCTGACTCT; CTGGGCTGTGATCGATTGGGCTGCTTTAACCTCAGCATCCGAGGGCATGGGGAATGCGTT; GAATATGTCAAGAGCTTCAATATCCCTCTACTCGTGCTGGGTGGTGGTGGTTATACTGTC; CGAAATGTTGCCCGCTGCTGGACATATGAGACATCGCTGCTGGTAGAAGAGGCCATTAGT; GAGGAGCTTCCCTATAGTGAATACTTCGAGTACTTTGCCCCAGACTTCACACTTCATCCA; GATGTCAGCACCCGCATCGAGAATCAGAACTCACGCCAGTATCTGGACCAGATCCGCCAG; ACAATCTTTGAAAACCTGAAGATGCTGAACCATGCACCTAGTGTCCAGATTCATGACGTG; CCTGCAGACCTCCTGACCTATGACAGGACTGATGAGGCTGATGCAGAGGAGAGGGGTCCT; GAGGAGAACTATAGCAGGCCAGAGGCACCCAATGAGTTCTATGATGGAGACCATGACAAT; GACAAGGAAAGCGATGTGGAGATTTAA; ; Clone variation with respect to NM_003883.3; 165 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003883 unedited; NNNCCTGTCANAAATTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACCAGGCCGC; GGGCGGCGGGCGGCGGAGGTGCGGGGCCTGCTCCCGCCGGCACCATGGCCAAGACCGTGG; CCTATTTCTACGACCCCGACGTGGGCAACTTCCACTACGGAGCTGGACACCCTATGAAGC; CCCATCGCCTGGCATTGACCCATAGCCTGGTCCTGCATTACGGTCTCTATAAGAAGATGA; TCGTCTTCAAGCCATACCAGGCCTCCCAGCATGACATGTGCCGCTTCCACTCCGAGGACT; ACATTGACTTCCTGCAGAGAGTCAGCCCCACCAATATGCAAGGCTTCACCAAGAGTCTTA; ATGCCTTCAACGTAGGCGATGACTGCCCAGTGTTTCCCGGGCTCTTTGAGTTCTGCTCGC; GTTACACAGGCGCATCTCTGCAAGGAGCAACCCAGCTGAACAACAAGATCTGTGATATTG; CCATTAACTGGGCTGGTGGTCTGCACCATGCCAAGAAGTTTGAGGCCTCTGGCTTCTGCT; ATGTCAACGACATTGTGATTGGCATCCTGGAGCTGCTCAAGTACCACCCTCGGGTGCTCT; ACATTGACATTGACATCCACCATGGTGACGGNGTTCAAGAAGCTTTCTACCTCACTGACC; GNGTCATGACGGTGTCCTTCCACAAATACGGAAATTACTTCTTCCCTGGCACAGGTGACA; TGTATGAAGTCGGNGCAGAGAGTGGCCGCTACTACTGTCTGAACGTGCCCCTGCGGNATG; GCATTGATGACCAGAGNTACAAGCACCTTTTCCAGCCGGNTATCAACCAGNTAGTGGACT; TCTACCAACCCACGTGCATTGTGCTTCAGTGTGGAGCTGACTCTCTGGCCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003883 unedited; CCAAAAGTGAGTTTCAGACATTTTCATATCCTCCCCACACTTGAAAACATACTTCCCATC; ATCTGGGATTCAGGTGTTAGGGAGCCAGAGCCCCTTCCAAATCTCTCTCTCTTCATCTTC; CCTGGAAGCAAGGGGAGGAAGAAGTCAGAACCCAGAGGATGAGGTANNTANAGCAATCTC; AGTCCTTGTCTACCCGTTCATCCCTCAAAAATCTCTGGGTTCGAGGGAAGCAGGGAAGAA; ATAAGGGGCAAGGGGGGCTAGGGACTGGCCTCCAGGGCCCACTGCCAATAATGTCCCAGA; TAGCTATCCTTGTCTGTATCTCATCCCTAATAGGTACCATTGTCAGGCCTTGGGAGAGAG; AGGAAAAGCAGGTAGATGGTTCGAGAACCAAATGTGGTCTCCAGACTCTTTCCCAGAGTC; AGCAAAAGCCCTGGGGTGAACCCCAGGACTCTAGGAGCCACTCCTTTTCCCTCCAGCCCA; ACCAAGAGGTGAAAAGAAATTCCTTGGGACACAGCATCCCAAGCCACTCTTAAATCTCCA; CATCGCTTTCCTTGTCATTGTCATGGTCTCCATCATAGAACTCATTGGGTGCCTCTGGCC; TGCTATAGTTCTCCTCAGGACCCCTCTCCTCTGCATCAGCCTCATCAGTCCTGTCATAGG; TCAGGAGGTCTGCAGGCACGTCATGAATCTGGACACTAGGTGCATGGTTCAGCATCTTCA; GGTTTTCAAAGATTGTCTGGCGGATCTGGTCCAGATACTGGCGTGAGTTCTGATTCTCGA; TGCCGGTGCTGACATCTGGATGAAGTGTGAAGTCTGGGGCAAAGTACTCGAAGTATTCCC; TAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003883
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003883.2, NP_003874.2
• RefSeq Size: 1955 bp
• RefSeq ORF: 1287 bp
• Locus ID: 8841
• UniProt ID: O15379
• Cytogenetics: 5q31.3
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Transcription Factors
• Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.