HSP90AA1 (NM_005348) Human Untagged Clone

SKU SC112678

HSP90AA1 (untagged)-Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: HSP90AA1
• Synonyms: EL52; HEL-S-65p; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; Hsp103; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112678 sequence for NM_005348 edited (data generated by NextGen Sequencing); ATGCCTGAGGAAACCCAGACCCAAGACCAACCGATGGAGGAGGAGGAGGTTGAGACGTTC; GCCTTTCAGGCAGAAATTGCCCAGTTGATGTCATTGATCATCAATACTTTCTACTCGAAC; AAAGAGATCTTTCTGAGAGAGCTCATTTCAAATTCATCAGATGCATTGGACAAAATCCGG; TATGAAAGCTTGACAGATCCCAGTAAATTAGACTCTGGGAAAGAGCTGCATATTAACCTT; ATACCGAACAAACAAGATCGAACTCTCACTATTGTGGATACTGGAATTGGAATGACCAAG; GCTGACTTGATCAATAACCTTGGTACTATCGCCAAGTCTGGGACCAAAGCGTTCATGGAA; GCTTTGCAGGCTGGTGCAGATATCTCTATGATTGGCCAGTTCGGTGTTGGTTTTTATTCT; GCTTATTTGGTTGCTGAGAAAGTAACTGTGATCACCAAACATAACGATGATGAGCAGTAC; GCTTGGGAGTCCTCAGCAGGGGGATCATTCACAGTGAGGACAGACACAGGTGAACCTATG; GGTCGTGGAACAAAAGTTATCCTACACCTGAAAGAAGACCAAACTGAGTACTTGGAGGAA; CGAAGAATAAAGGAGATTGTGAAGAAACATTCTCAGTTTATTGGATATCCCATTACTCTT; TTTGTGGAGAAGGAACGTGATAAAGAAGTAAGCGATGATGAGGCTGAAGAAAAGGAAGAC; AAAGAAGAAGAAAAAGAAAAAGAAGAGAAAGAGTCGGAAGACAAACCTGAAATTGAAGAT; GTTGGTTCTGATGAGGAAGAAGAAAAGAAGGATGGTGACAAGAAGAAGAAGAAGAAGATT; AAGGAAAAGTACATCGATCAAGAAGAGCTCAACAAAACAAAGCCCATCTGGACCAGAAAT; CCCGACGATATTACTAATGAGGAGTACGGAGAATTCTATAAGAGCTTGACCAATGACTGG; GAAGATCACTTGGCAGTGAAGCATTTTTCAGTTGAAGGACAGTTGGAATTCAGAGCCCTT; CTATTTGTCCCACGACGTGCTCCTTTTGATCTGTTTGAAAACAGAAAGAAAAAGAACAAY; ATCAAATTGTATGTACGCAGAGTTTTCATCATGGATAACTGTGAGGAGCTAATCCCTGAA; TATCTGAACTTCATTAGAGGGGTGGTAGACTCGGAGGATCTCCCTCTAAACATATCCCGT; GAGATGTTGCAACAAAGCAAAATTTTGAAAGTTATCAGGAAGAATTTGGTCAAAAAATGC; TTAGAACTCTTTACTGAACTGGCGGAAGATAAAGAGAACTACAAGAAATTCTATGAGCAG; TTCTCTAAAAACATAAAGCTTGGAATACACGAAGACTCTCAAAATCGGAAGAAGCTTTCA; GAGCTGTTAAGGTACTACACATCTGCCTCTGGTGATGAGATGGTTTCTCTCAAGGACTAC; TGCACCAGAATGAAGGAGAACCAGAAACATATCTATTATATCACAGGTGAGACCAAGGAC; CAGGTAGCTAACTCAGCCTTTGTGGAACGTCTTCGGAAACATGGCTTAGAAGTGATCTAT; ATGATTGAGCCCATTGATGAGTACTGTGTCCAACAGCTGAAGGAATTTGAGGGGAAGACT; TTAGTGTCAGTCACCAAAGAAGGCCTGGAACTTCCAGAGGATGAAGAAGAGAAAAAGAAG; CAGGAAGAGAAAAAAACAAAGTTTGAGAACCTCTGCAAAATCATGAAAGACATATTGGAG; AAAAAAGTTGAAAAGGTGGTTGTGTCAAACCGATTGGTGACATCTCCATGCTGTATTGTC; ACAAGCACATATGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCTAAGA; GACAACTCAACAATGGGTTACATGGCAGCAAAGAAACACCTGGAGATAAACCCTGACCAT; TCCATTATTGAGACCTTAAGGCAAAAGGCAGAGGCTGATAAGAACGACAAGTCTGTGAAG; GATCTGGTCATCTTGCTTTATGAAACTGCGCTCCTGTCTTCTGGCTTCAGTCTGGAAGAT; CCCCAGACACATGCTAACAGGATCTACAGGATGATCAAACTTGGTCTGGGTATTGATGAA; GATGACCCTACTGCTGATGATACCAGTGCTGCTGTAACTGAAGAAATGCCACCCCTTGAA; GGAGATGACGACACATCACGCATGGAAGAAGTAGACTAA; ; Clone variation with respect to NM_005348.3; 1080 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005348 unedited; GCCTATTCCCACGAGGGCTGTGCTGTTGGTCCTGTGCGGTCACTTAGCCAATTGCCTGAG; GAAACCCATACCCAANACCAACCGATGGAGGAGGAGGAGGTTGAGACGTTCGCCTTTCAG; CAGAATTGCCCAGTTGTGTCATTGTCATCAATACTTTCTACTCGTCAAATAGATTTTCTG; AGAGAGCTCATTTCAAATTCATCAGATGCATTGGACAAAATCCGGTATGAAAGCTTGACA; GATCCCAGTAAATTATACTCTGGGAAAGAGCTGCATATTAACCTTATACCGAACAAACAA; GATCGAACTCTCACTATTGTGGATACTGGAATTGGAATGACCAAGGCTGACTTGATCAAT; AACCTTGGTACTATCGCCAAGTCTGGGACCAAAGCGTTCATGGAAGCTTTGCAGGCTGGT; GCAGATATCTCTATGATTGGCCAGTTCGGTGTTGGTTTTTATTCTGCTTATTTGGTTGCT; GAGAAAGTAACTGTGATCACCAAACATAACGATGATGAGCAGTACGCTTGGGAGTCCTCA; GCAGGGGGATCATTCACAGTGAGGACAGACACAGGTGAACCTATGGGTCGTGGAACAAAA; GTTATCCTACACCTGAAAGAAGACCAAACTGAGTACTTGGAGGAACGAAGAATAAAGGAG; ATTGTGAAGAAACATTCTCAGTTTATTGGATATCCCATTACTCTTTTTGTGGAGAAGGGA; CGTGATTAAGAAGTAAGCGATGATGAGGCCTGAGAAAAGGAAGACAAGNAGGAGAAAAAG; AAAAGAGGAGAAGAGTCGGAAAACA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005348 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTAACT; CATCTGTATTTGTTACAACTTTAAGCAAATGGGACTCGAGCACTACATTTCCATCCACAA; GACTGGGTCTGAGTTATTTTTGAACAGCTTTACGATATGCTTAGGTAGGCTTTTAACTTT; GCTCCTCCAAACAATACTTTTCTTTGGAAAACAAGCCCTGGGGAGAGATCCTTCCATCAA; GTTGCTTCAGTTTAACCTATTTCTAGAGGACTAGTACATGCAGAATTGTCAACTACAGGG; AATGAAAAGTTCGAAAAGTAGATCCTACAAGATGTAACGAATACTTTTCTAAACATCAAG; ATACAGCTCAAAACACTTCAATAACAAGATTTGGTCTACTTAGGCATCCGGCTTGACAGC; TAAACACTTTAGACCACAAAGTTAACATCATGTTACATACGTCTTACAGGGCACGTTACC; CCAATCTGTGAAAATAAACCAACATGAAACTCAAAAAGCATTACTAGCTCTGCTTTAGTG; CCTAAGGTATCACAGCATCACTTAGTAGACAGAAATCTTATCTTCCCCTTAAAGTAGTTG; TCATGCCATACAGACTTTTTAATATTACAAAAATAAAGAAAAACATCCTTGAAAATATAT; TATCAGAGGAATTGTAGAGTACTGAACAGGTAAGTCATCCCTCAGCCAGAGATTAGTCTA; CTTCTTCCATGCGGGATGTGTCGTCATCTCCTTCAGGGGTGGCATTTCTTCATTACAGCA; GCACTGGTATATCAGCAGTAGGGTCATCTTCATCATACCCAGACAAGNNTGATCTCCTGT; ANAATCTGTANCATGTGTCTGGGATCTTCAACTGAGCCANAGACAGACGCAGTTCTAAGC; AGAGACAGATCTTCAGACTTGCGTCTATAGCTTGCCTTGCCTAGGCTATATGGAGGGAGG; TTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005348
• Insert Size: 2950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005348.2, NP_005339.2
• RefSeq Size: 2912 bp
• RefSeq ORF: 2199 bp
• Locus ID: 3320
• UniProt ID: P07900
• Cytogenetics: 14q32.31
• Domains: HATPase_c, HSP90
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer
• Summary: The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]; Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.