HSP90AA1 (NM_005348) Human Untagged Clone

CAT#: SC112678

HSP90AA1 (untagged)-Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSP90AA1
• Synonyms: EL52; HEL-S-65p; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; Hsp103; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112678 sequence for NM_005348 edited (data generated by NextGen Sequencing)
  ATGCCTGAGGAAACCCAGACCCAAGACCAACCGATGGAGGAGGAGGAGGTTGAGACGTTC
  GCCTTTCAGGCAGAAATTGCCCAGTTGATGTCATTGATCATCAATACTTTCTACTCGAAC
  AAAGAGATCTTTCTGAGAGAGCTCATTTCAAATTCATCAGATGCATTGGACAAAATCCGG
  TATGAAAGCTTGACAGATCCCAGTAAATTAGACTCTGGGAAAGAGCTGCATATTAACCTT
  ATACCGAACAAACAAGATCGAACTCTCACTATTGTGGATACTGGAATTGGAATGACCAAG
  GCTGACTTGATCAATAACCTTGGTACTATCGCCAAGTCTGGGACCAAAGCGTTCATGGAA
  GCTTTGCAGGCTGGTGCAGATATCTCTATGATTGGCCAGTTCGGTGTTGGTTTTTATTCT
  GCTTATTTGGTTGCTGAGAAAGTAACTGTGATCACCAAACATAACGATGATGAGCAGTAC
  GCTTGGGAGTCCTCAGCAGGGGGATCATTCACAGTGAGGACAGACACAGGTGAACCTATG
  GGTCGTGGAACAAAAGTTATCCTACACCTGAAAGAAGACCAAACTGAGTACTTGGAGGAA
  CGAAGAATAAAGGAGATTGTGAAGAAACATTCTCAGTTTATTGGATATCCCATTACTCTT
  TTTGTGGAGAAGGAACGTGATAAAGAAGTAAGCGATGATGAGGCTGAAGAAAAGGAAGAC
  AAAGAAGAAGAAAAAGAAAAAGAAGAGAAAGAGTCGGAAGACAAACCTGAAATTGAAGAT
  GTTGGTTCTGATGAGGAAGAAGAAAAGAAGGATGGTGACAAGAAGAAGAAGAAGAAGATT
  AAGGAAAAGTACATCGATCAAGAAGAGCTCAACAAAACAAAGCCCATCTGGACCAGAAAT
  CCCGACGATATTACTAATGAGGAGTACGGAGAATTCTATAAGAGCTTGACCAATGACTGG
  GAAGATCACTTGGCAGTGAAGCATTTTTCAGTTGAAGGACAGTTGGAATTCAGAGCCCTT
  CTATTTGTCCCACGACGTGCTCCTTTTGATCTGTTTGAAAACAGAAAGAAAAAGAACAAY
  ATCAAATTGTATGTACGCAGAGTTTTCATCATGGATAACTGTGAGGAGCTAATCCCTGAA
  TATCTGAACTTCATTAGAGGGGTGGTAGACTCGGAGGATCTCCCTCTAAACATATCCCGT
  GAGATGTTGCAACAAAGCAAAATTTTGAAAGTTATCAGGAAGAATTTGGTCAAAAAATGC
  TTAGAACTCTTTACTGAACTGGCGGAAGATAAAGAGAACTACAAGAAATTCTATGAGCAG
  TTCTCTAAAAACATAAAGCTTGGAATACACGAAGACTCTCAAAATCGGAAGAAGCTTTCA
  GAGCTGTTAAGGTACTACACATCTGCCTCTGGTGATGAGATGGTTTCTCTCAAGGACTAC
  TGCACCAGAATGAAGGAGAACCAGAAACATATCTATTATATCACAGGTGAGACCAAGGAC
  CAGGTAGCTAACTCAGCCTTTGTGGAACGTCTTCGGAAACATGGCTTAGAAGTGATCTAT
  ATGATTGAGCCCATTGATGAGTACTGTGTCCAACAGCTGAAGGAATTTGAGGGGAAGACT
  TTAGTGTCAGTCACCAAAGAAGGCCTGGAACTTCCAGAGGATGAAGAAGAGAAAAAGAAG
  CAGGAAGAGAAAAAAACAAAGTTTGAGAACCTCTGCAAAATCATGAAAGACATATTGGAG
  AAAAAAGTTGAAAAGGTGGTTGTGTCAAACCGATTGGTGACATCTCCATGCTGTATTGTC
  ACAAGCACATATGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCTAAGA
  GACAACTCAACAATGGGTTACATGGCAGCAAAGAAACACCTGGAGATAAACCCTGACCAT
  TCCATTATTGAGACCTTAAGGCAAAAGGCAGAGGCTGATAAGAACGACAAGTCTGTGAAG
  GATCTGGTCATCTTGCTTTATGAAACTGCGCTCCTGTCTTCTGGCTTCAGTCTGGAAGAT
  CCCCAGACACATGCTAACAGGATCTACAGGATGATCAAACTTGGTCTGGGTATTGATGAA
  GATGACCCTACTGCTGATGATACCAGTGCTGCTGTAACTGAAGAAATGCCACCCCTTGAA
  GGAGATGACGACACATCACGCATGGAAGAAGTAGACTAA
  
  Clone variation with respect to NM_005348.3
  1080 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005348 unedited
  GCCTATTCCCACGAGGGCTGTGCTGTTGGTCCTGTGCGGTCACTTAGCCAATTGCCTGAG
  GAAACCCATACCCAANACCAACCGATGGAGGAGGAGGAGGTTGAGACGTTCGCCTTTCAG
  CAGAATTGCCCAGTTGTGTCATTGTCATCAATACTTTCTACTCGTCAAATAGATTTTCTG
  AGAGAGCTCATTTCAAATTCATCAGATGCATTGGACAAAATCCGGTATGAAAGCTTGACA
  GATCCCAGTAAATTATACTCTGGGAAAGAGCTGCATATTAACCTTATACCGAACAAACAA
  GATCGAACTCTCACTATTGTGGATACTGGAATTGGAATGACCAAGGCTGACTTGATCAAT
  AACCTTGGTACTATCGCCAAGTCTGGGACCAAAGCGTTCATGGAAGCTTTGCAGGCTGGT
  GCAGATATCTCTATGATTGGCCAGTTCGGTGTTGGTTTTTATTCTGCTTATTTGGTTGCT
  GAGAAAGTAACTGTGATCACCAAACATAACGATGATGAGCAGTACGCTTGGGAGTCCTCA
  GCAGGGGGATCATTCACAGTGAGGACAGACACAGGTGAACCTATGGGTCGTGGAACAAAA
  GTTATCCTACACCTGAAAGAAGACCAAACTGAGTACTTGGAGGAACGAAGAATAAAGGAG
  ATTGTGAAGAAACATTCTCAGTTTATTGGATATCCCATTACTCTTTTTGTGGAGAAGGGA
  CGTGATTAAGAAGTAAGCGATGATGAGGCCTGAGAAAAGGAAGACAAGNAGGAGAAAAAG
  AAAAGAGGAGAAGAGTCGGAAAACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005348 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTAACT
  CATCTGTATTTGTTACAACTTTAAGCAAATGGGACTCGAGCACTACATTTCCATCCACAA
  GACTGGGTCTGAGTTATTTTTGAACAGCTTTACGATATGCTTAGGTAGGCTTTTAACTTT
  GCTCCTCCAAACAATACTTTTCTTTGGAAAACAAGCCCTGGGGAGAGATCCTTCCATCAA
  GTTGCTTCAGTTTAACCTATTTCTAGAGGACTAGTACATGCAGAATTGTCAACTACAGGG
  AATGAAAAGTTCGAAAAGTAGATCCTACAAGATGTAACGAATACTTTTCTAAACATCAAG
  ATACAGCTCAAAACACTTCAATAACAAGATTTGGTCTACTTAGGCATCCGGCTTGACAGC
  TAAACACTTTAGACCACAAAGTTAACATCATGTTACATACGTCTTACAGGGCACGTTACC
  CCAATCTGTGAAAATAAACCAACATGAAACTCAAAAAGCATTACTAGCTCTGCTTTAGTG
  CCTAAGGTATCACAGCATCACTTAGTAGACAGAAATCTTATCTTCCCCTTAAAGTAGTTG
  TCATGCCATACAGACTTTTTAATATTACAAAAATAAAGAAAAACATCCTTGAAAATATAT
  TATCAGAGGAATTGTAGAGTACTGAACAGGTAAGTCATCCCTCAGCCAGAGATTAGTCTA
  CTTCTTCCATGCGGGATGTGTCGTCATCTCCTTCAGGGGTGGCATTTCTTCATTACAGCA
  GCACTGGTATATCAGCAGTAGGGTCATCTTCATCATACCCAGACAAGNNTGATCTCCTGT
  ANAATCTGTANCATGTGTCTGGGATCTTCAACTGAGCCANAGACAGACGCAGTTCTAAGC
  AGAGACAGATCTTCAGACTTGCGTCTATAGCTTGCCTTGCCTAGGCTATATGGAGGGAGG
  TTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005348
• Insert Size: 2950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005348.2, NP_005339.2
• RefSeq Size: 2912 bp
• RefSeq ORF: 2199 bp
• Locus ID: 3320
• UniProt ID: P07900
• Cytogenetics: 14q32.31
• Domains: HSP90, HATPase_c
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer
• Gene Summary: The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.