Sulfatase 1 (SULF1) (NM_015170) Human Untagged Clone

SKU SC111204

SULF1 (untagged)-Human sulfatase 1 (SULF1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: Sulfatase 1
• Synonyms: SULF-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015170, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAAGTATTCTTGCTGTGCTCTGGTTTTGGCTGTCCTGGGCACAGAATTGCTGGGAAGCCTCTGTTCGA; CTGTCAGATCCCCGAGGTTCAGAGGACGGATACAGCAGGAACGAAAAAACATCCGACCCAACATTATTCT; TGTGCTTACCGATGATCAAGATGTGGAGCTGGGGTCCCTGCAAGTCATGAACAAAACGAGAAAGATTATG; GAACATGGGGGGGCCACCTTCATCAATGCCTTTGTGACTACACCCATGTGCTGCCCGTCACGGTCCTCCA; TGCTCACCGGGAAGTATGTGCACAATCACAATGTCTACACCAACAACGAGAACTGCTCTTCCCCCTCGTG; GCAGGCCATGCATGAGCCTCGGACTTTTGCTGTATATCTTAACAACACTGGCTACAGAACAGCCTTTTTT; GGAAAATACCTCAATGAATATAATGGCAGCTACATCCCCCCTGGGTGGCGAGAATGGCTTGGATTAATCA; AGAATTCTCGCTTCTATAATTACACTGTTTGTCGCAATGGCATCAAAGAAAAGCATGGATTTGATTATGC; AAAGGACTACTTCACAGACTTAATCACTAACGAGAGCATTAATTACTTCAAAATGTCTAAGAGAATGTAT; CCCCATAGGCCCGTTATGATGGTGATCAGCCACGCTGCGCCCCACGGCCCCGAGGACTCAGCCCCACAGT; TTTCTAAACTGTACCCCAATGCTTCCCAACACATAACTCCTAGTTATAACTATGCACCAAATATGGATAA; ACACTGGATTATGCAGTACACAGGACCAATGCTGCCCATCCACATGGAATTTACAAACATTCTACAGCGC; AAAAGGCTCCAGACTTTGATGTCAGTGGATGATTCTGTGGAGAGGCTGTATAACATGCTCGTGGAGACGG; GGGAGCTGGAGAATACTTACATCATTTACACCGCCGACCATGGTTACCATATTGGGCAGTTTGGACTGGT; CAAGGGGAAATCCATGCCATATGACTTTGATATTCGTGTGCCTTTTTTTATTCGTGGTCCAAGTGTAGAA; CCAGGATCAATAGTCCCACAGATCGTTCTCAACATTGACTTGGCCCCCACGATCCTGGATATTGCTGGGC; TCGACACACCTCCTGATGTGGACGGCAAGTCTGTCCTCAAACTTCTGGACCCAGAAAAGCCAGGTAACAG; GTTTCGAACAAACAAGAAGGCCAAAATTTGGCGTGATACATTCCTAGTGGAAAGAGGCAAATTTCTACGT; AAGAAGGAAGAATCCAGCAAGAATATCCAACAGTCAAATCACTTGCCCAAATATGAACGGGTCAAAGAAC; TATGCCAGCAGGCCAGGTACCAGACAGCCTGTGAACAACCGGGGCAGAAGTGGCAATGCATTGAGGATAC; ATCTGGCAAGCTTCGAATTCACAAGTGTAAAGGACCCAGTGACCTGCTCACAGTCCGGCAGAGCACGCGG; AACCTCTACGCTCGCGGCTTCCATGACAAAGACAAAGAGTGCAGTTGTAGGGAGTCTGGTTACCGTGCCA; GCAGAAGCCAAAGAAAGAGTCAACGGCAATTCTTGAGAAACCAGGGGACTCCAAAGTACAAGCCCAGATT; TGTCCATACTCGGCAGACACGTTCCTTGTCCGTCGAATTTGAAGGTGAAATATATGACATAAATCTGGAA; GAAGAAGAAGAATTGCAAGTGTTGCAACCAAGAAACATTGCTAAGCGTCATGATGAAGGCCACAAGGGGC; CAAGAGATCTCCAGGCTTCCAGTGGTGGCAACAGGGGCAGGATGCTGGCAGATAGCAGCAACGCCGTGGG; CCCACCTACCACTGTCCGAGTGACACACAAGTGTTTTATTCTTCCCAATGACTCTATCCATTGTGAGAGA; GAACTGTACCAATCGGCCAGAGCGTGGAAGGACCATAAGGCATACATTGACAAAGAGATTGAAGCTCTGC; AAGATAAAATTAAGAATTTAAGAGAAGTGAGAGGACATCTGAAGAGAAGGAAGCCTGAGGAATGTAGCTG; CAGTAAACAAAGCTATTACAATAAAGAGAAAGGTGTAAAAAAGCAAGAGAAATTAAAGAGCCATCTTCAC; CCATTCAAGGAGGCTGCTCAGGAAGTAGATAGCAAACTGCAACTTTTCAAGGAGAACAACCGTAGGAGGA; AGAAGGAGAGGAAGGAGAAGAGACGGCAGAGGAAGGGGGAAGAGTGCAGCCTGCCTGGCCTCACTTGCTT; CACGCATGACAACAACCACTGGCAGACAGCCCCGTTCTGGAACCTGGGATCTTTCTGTGCTTGCACGAGT; TCTAACAATAACACCTACTGGTGTTTGCGTACAGTTAATGAGACGCATAATTTTCTTTTCTGTGAGTTTG; CTACTGGCTTTTTGGAGTATTTTGATATGAATACAGATCCTTATCAGCTCACAAATACAGTGCACACGGT; AGAACGAGGCATTTTGAATCAGCTACACGTACAACTAATGGAGCTCAGAAGCTGTCAAGGATATAAGCAG; TGCAACCCAAGACCTAAGAATCTTGATGTTGGAAATAAAGATGGAGGAAGCTATGACCTACACAGAGGAC; AGTTATGGGATGGATGGGAAGGTTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015170 unedited; TAGATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGACGGAGA; CATTTTGTCAGTTTTGCAACATTGGACCAAATACAATGAAGTATTCTTGCTGTGCTCTGG; TTTTGGCTGTCCTGGGCACAGAATTGCTGGGAAGCCTCTGTTCGACTGTCAGATCCCCGA; GGTTCAGAGGACGGATACAGCAGGAACGAAAAAACATCCGACCCAACATTATTCTTGTGC; TTACCGATGATCAAGATGTGGAGCTGGGGTCCCTGCAAGTCATGAACAAAACGAGAAAGA; TTATGGAACATGGGGGGGCCACCTTCATCAATGCCTTTGTGACTACACCCATGTGCTGCC; CGTCACGGTCCTCCATGCTCACCGGGAAGTATGTGCACAATCACAATGTCTACACCAACA; ACGAGAACTGCTCTTCCCCCTCGTGGCAGGCCATGCATGAGCCTCGGACTTTTGCTGTAT; ATCTTAACAACACTGGCTACAGAACAGCCTTTTTTGGAAAATACCTCAATGAATATAATG; GCAGCTACATCCCCCCTGNGTGGCGAGAATGGCTTGGATTAATCAAGAATTCTCGCTTCT; ATAATTACACTGTTTGTCGCAATGGCATCAAAGAAAAGCATGGATTTGATTATGCAAAGG; ACTACTTCACAGACTTAATCACTAACGAGAGCATTAATTACTTCAAAATGTCTAAGAGAA; TGTATCCCCATAGGCCCGTTATGATGGTGATCAGCCACGCTGCGCCCCACGGCCCCGAGG; ACTCAGCCCCACAGTTTTCTAAACTGTACCCCATGCTTTCCCACACATAACTCCTAGTTA; TAACTATGCACCAAATATGGGATAAACCCTGGNATTATGCAGTACACAGGGACCAATGCT; GCCCATCCACATGGANATTTACCAACATTCTACAGGGCAAAAGGCTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015170 unedited; ATCTTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTTTACTTA; TTTATTTATTTTCACCACCAACATTATTAGCCATGCCTTTCTGCTAATCGATTTTAGCAA; GTCGAGGTAAAACACATGCAACATTTTCTGGCAAAAGCTTAATGTCAAACAATATGTGAT; CCATACTGTGTGTCGTCCTTGGGGGTTTATTTGACTTTGTCACAATGACAGCCAACAGTG; AGACTGATAAGCCTGTAAAAATAAAAAAATAAGACTAATCAAATAGACATGGCATTTTAA; TCTCAAAGTGCAAAATCATCTAACTGAAAATGACGGCATTGAAAAATTCCAGTGGTTAAA; AATGAATCAAAACTTCATTACGCAGGCAGTGGAAGTGTGTTGAAAGATTTACCAGGGGTG; TCAAGTTTTAGACACTCAGAAAGGCACCATTCTAGCCATCTTGATTGGATAACATGTATA; TACTTATGTCCCTACGATATTCAAAAGATAATACTGTTTTAGTACAAAACAAACAAACAA; GCAAAAAATCAAAACCAAGCCAACCCAAATATCCCCAGCCTTTCTTTCTACTCTTGGCAG; ATAGTAAATTATAACGATGAGTCTCCGTGTGCACACCGCTTGCTCACATGCTCACTAGCT; TCTACTGCACAAAGGTACCCAGGGTAGCTTGGAAATGTTGTGGCTGTGAATACCTTTTAT; AGNNTACAAATAAAAAGTTAANAAAGAATACTGTGNTTNAGGNNTAAGTANCAGTTTCAT; CTAATCANNAGAGAGTGAANAAGAAGCGCTGCCTTTCTAGNGCTGNGACTTCTCCTTTTC; GNNGATCTCTTCACCTTGTCAACATCTTNCCCGCTATGCTGAAATACTTNGNGTTCCTGC; GTGGCCATGGGGAACATCTGATGAACTGAATNTNCATCGNATN;
• Restriction Sites: NotI-NotI
• ACCN: NM_015170
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015170.1, NP_055985.1
• RefSeq Size: 5699 bp
• RefSeq ORF: 2616 bp
• Locus ID: 23213
• UniProt ID: Q8IWU6
• Cytogenetics: 8q13.2-q13.3
• Protein Families: Druggable Genome
• Summary: This gene encodes an extracellular heparan sulfate endosulfatase. The encoded enzyme selectively removes 6-O-sulfate groups from heparan sulfate chains of heparan sulfate proteoglycans (HSPGs). The enzyme is secreted through the Golgi and is subsequently localized to the cell surface. The expression of this gene may be down-regulated in several types of cancer, including hepatocellular (HCC), ovarian and breast cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]; Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein.