ELOB (NM_007108) Human Untagged Clone

SKU SC111036

TCEB2 (untagged)-Human transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B) (TCEB2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ELOB
• Synonyms: SIII; TCEB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111036 sequence for NM_007108 edited (data generated by NextGen Sequencing); ATGGACGTGTTCCTCATGATCCGGCGCCACAAGACCACCATCTTCACGGACGCCAAGGAG; TCCAGCACGGTGTTCGAACTGAAGCGCATCGTCGAGGGCATCCTCAAGCGGCCTCCTGAC; GAGCAGCGGCTGTACAAGGATGACCAACTCTTGGATGATGGCAAGACACTGGGCGAGTGT; GGCTTCACCAGTCAAACAGCACGGCCACAGGCCCCAGCCACAGTGGGGCTGGCCTTCCGG; GCAGATGACACCTTTGAGGCCCTGTGCATCGAGCCGTTTTCCAGCCCGCCAGAGCTGCCC; GATGTGATGAAGCCCCAGGACTCGGGAAGCAGTGCCAATGAACAAGCCGTGCAGTGA; ; Clone variation with respect to NM_007108.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007108 unedited; TCCGTGCAAAATTTGTNATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGGCA; GCAGCCGCGATGGACGTGTTCCTCATGATCCGGCGCCACAAGACCACCATCTTCACGGAC; GCCAAGGAGTCCAGCACGGTGTTCGAACTGAAGCGCATCGTCGAGGGCATCCTCAAGCGG; CCTCCTGACGAGCAGCGGCTGTACAAGGATGACCAACTCTTGGATGATGGCAAGACACTG; GGCGAGTGTGGCTTCACCAGTCAAACAGCACGGCCACAGGCCCCAGCCACAGTGGGGCTG; GCCTTCCGGGCAGATGACACCTTTGAGGCCCTGTGCATCGAGCCGTTTTCCAGCCCGCCA; GAGCTGCCCGATGTGATGAAGCCCCAGGACTCGGGAAGCAGTGCCAATGAACAAGCCGTG; CAGTGAGACCCCCAAGAGGCCCATTTCCCCCAATAAAAGAGATTTGGGAGTCTGAAAAAA; AAAAACCACCCAAATCAAAATACCCAAAAAACACCAAATACCAAAAAAAAACAAAAAACC; CTCGCCTTTAAATTGGGGCCGGGGTCATACTTGTTTCCTGAACAAATCCCGGGGGGCATC; CCTGTGACCCCTCCCAGTGCCTTTCCTGGCCTTGAAAGTGGCCATTCCAGGGCCCACCAG; CCTTGCCCTATTAAAATTAAGTGGCATCATTTTGTCTGACTAGGGGCCCTTCTATAATAT; AATGGGGTGAGGGGGCGTGGGTTATGACAACCCCGTTTCCATCTTCCCCATAACCCCTTT; TCCTGCCCCGCGGGTTCCTCTTTCCCCCATATCTCTGATATCGCTTCCCAAACTTCTTGC; ACTCCCGCATTCCTCCCCCCTTCGGCCCTACCCATCCTCTTCCTACTCTACCACCTCCGC; TCCCCCTCATTTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007108 unedited; CGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTAAAAACCCCCAAATTTTTTTTATTGGGGAAAATGG; CCCTTTTGGGGGTCTAACTGCACGGTTTGTTAATTGGCACTGTTTCCCAAGTCCTGGGGC; TTAATAAAATCGGGCAATTTTGGGGGGCTGAAAAACGGTTCAATCCACAGGGCCTAAAAG; GGGTCATTTCCCCGAAAGGCCACCCCCACTGGGGCTGGGCCCTGGGCCCCTCCTTTTTAA; CTGGAAAACCCACCCTCCCCCAGGGTTTTGCCATCATCCAAAAGTTGGTCATCCTTGAAC; ACCCCCTGCTCTTAAAAAGGCCCTTTGAGGATCCCCTCAACAATGCCCTTAATTTAAAAC; ACCGGGCTGAACTCCTTGGCGCCCGAAAAAATGGGGGTTTTGGGGCCCCGAATAATAAAA; AACACTTCCATCGCGGTTGCTGCCTCCTCGGGCCAAATTCCCGGCCCCCCTATAGGGAGC; CGTATAACAAAATTTTAACGGTTCACTAAACAACCTTTGTTTATATAAACCTCCCACCGT; ACACCCCTACCGCCCATTTGCGTAAACGGGGCGGGGTTATTACAACATTTTGAAAAGCCC; CGTTGATTTTGGGCCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAAACTTGAGAAT; CCCCGTAAGTCAAACCGTTATCCCCGCCCATTGGTGTCACTGCCAAAACCCCATCCACCA; TGGAAATAACCGATGACCTAAACTAAATGCACTGCCCAATAAGAAAACCCCCTAGGTCAT; GTCCTGGGCAATATGCAGGCGGGCCATTTACCGTAATTACCACAATAGGGGCGACCTTGC; ATAAAAACCTTGAGTCCTGCAAGGGCCTTTCACCAAAACCCCCCATGACGCAAGGAAAGC; CCTAGGCCTCATTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_007108
• Insert Size: 540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007108.2, NP_009039.1
• RefSeq Size: 998 bp
• RefSeq ORF: 357 bp
• Locus ID: 6923
• UniProt ID: Q15370
• Cytogenetics: 16p13.3
• Domains: UBQ
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
• Summary: This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008]; Transcript Variant: This variant (1) encodes the predominant isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.