Prohibitin (PHB) (NM_002634) Human Untagged Clone

CAT#: SC110973

PHB (untagged)-Human prohibitin (PHB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Prohibitin
• Synonyms: HEL-215; HEL-S-54e; PHB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002634 edited
  GAATTCGGCACGAGGGGAATTCATGTGGAGGTCAGAGTGGAAGCAGGTGTGAGAGGGTCC
  AGCAGAAGGAAACATGGCTGCCAAAGTGTTTGAGTCCATTGGCAAGTTTGGCCTGGCCTT
  AGCTGTTGCAGGAGGCGTGGTGAACTCTGCCTTATATAATGTGGATGCTGGGCACAGAGC
  TGTCATCTTTGACCGATTCCGTGGAGTGCAGGACATTGTGGTAGGGGAAGGGACTCATTT
  TCTCATCCCGTGGGTACAGAAACCAATTATCTTTGACTGCCGTTCTCGACCACGTAATGT
  GCCAGTCATCACTGGTAGCAAAGATTTACAGAATGTCAACATCACACTGCGCATCCTCTT
  CCGGCCTGTCGCCAGCCAGCTTCCTCGCATCTTCACCAGCATCGGAGAGGACTATGATGA
  GCGTGTGCTGCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTTGATGC
  TGGAGAACTAATCACCCAGAGAGAGCTGGTCTCCAGGCAGGTGAGCGACGACCTTACAGA
  GCGAGCCGCCACCTTTGGGCTCATCCTGGATGACGTGTCCTTGACACATCTGACCTTCGG
  GAAGGAGTTCACAGAAGCGGTGGAAGCCAAACAGGTGGCTCAGCAGGAAGCAGAGAGGGC
  CAGATTTGTGGTGGAAAAGGCTGAGCAACAGAAAAAGGCGGCCATCATCTCTGCTGAGGG
  CGACTCCAAGGCAGCTGAGCTGATTGCCAACTCACTGGCCACTGCAGGGGATGGCCTGAT
  CGAGCTGCGCAAGCTGGAAGCTGCAGAGGACATCGCGTACCAGCTCTCACGCTCTCGGAA
  CATCACCTACCTGCCAGCGGGGCAGTCCGTGCTCCTCCAGCTGCCCCAGTGAGGGCCCAC
  CCTGCCTGCACCTCCGCGGGCTGACTGGGCCACAGCCCCGATGATTCTTAACACAGCCTT
  CCTTCTGCTCCCACCCCAGAAATCACTGTGAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002634 unedited
  GCGTCAGGAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTC
  GTGCCGAATTCGGCACGAGGGGAATTCATGTGGAGGTCAGAGTGGAAGCAGGTGTGAGAG
  GGTCCAGCAGAAGGAAACATGGCTGCCAAAGTGTTTGAGTCCATTGGCAAGTTTGGCCTG
  GCCTTAGCTGTTGCAAGAGGCGTGGTGAACTCTGCCTTATATAATGTGGATGCTGGGCAC
  AGAGCTGTCATCTTTGACCGATTCCGTGGAGTGCAGGACATTGTGGTAGGGGAAGGGACT
  CATTTTCTCATCCCGTGGGTACAGAAACCAATTATCTTTGACTGCCGTTCTCGACCACGT
  AATGTGCCAGTCATCACTGGTAGCAAAGATTTACAGAATGTCAACATCACACTGCGCATC
  CTCTTCCGGCCTGTCGCCAGCCAGCTTCCTCGCATCTTCACCAGCATCGGAGAGGACTAT
  GATGAGCGTGTGCTGCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTT
  GATGCTGGAGAACTAATCACCCAGAGAGAGCTGGTCTCCAGGCAGGTGAGCGACGACCTT
  ACAGAGCGAGCCGCCACCTTTGGGCTCATCCTGGATGACGTGTCCTTGACACATCTGACC
  TTCGGGAAGGAGTTCACAGAAGCGGTGGAAGCCAAACAGGTGGCTCATCATGAAGCAGAG
  AGGGCCAGATTTGTGGTGGAAAATGCTGAGNCACAGAAAAGGCGGCCATCATCTCTGCTG
  NAGGGCGACTCCAGGCAGCTGAGCTGATTGCAACTCACTGGCACTGCAGGGGATGGCCTG
  ATCGAGCTGCGCAAGCTGGAAGCTGCAGAGGACATCGCGTACCAGCTCTAACGCTCTCGG
  GACATCACCTACCTGGCAGCGGGGCATGTCGTGCNTCTNCAGCTGCCCCAGTGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002634 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAGTGATTTTACCTTTATTT
  CCTTCACTTTAAGCCAATCATGAAATTTCACAGTGATTTCTGGGGTGGGAGCAGAAGGAA
  GGCTGTGTTAAGAATCATCGGGGCTGTGGCCCAGTCAGCCCGCGGAGGTGCAGGCAGGGT
  GGGCCCTCACTGGGGCAGCTGGAGGAGCACGGACTGCCCCGCTGGCAGGTAGGTGATGTT
  CCGAGAGCGTGAGAGCTGGTACGCGATGTCCTCTGCAGCTTCCAGCTTGCGCAGCTCGAT
  CAGGCCATCCCCTGCAGTGGCCAGTGAGTTGGCAATCAGCTCAGCTGCCTTGGAGTCGCC
  CTCAGCAGAGATGATGGCCGCCTTTTTCTGTTGCTCAGCCTTTTCCACCACAAATCTGGC
  CCTCTCTGCTTCCTGCTGAGCCACCTGTTTGGGCTCCACCGCTTCTTGGGACTCCCTTTC
  CGAAGGCAAAAAGGTTAAAGGCCCCCTTTTCCCCAAAAGACCCACAGGGGGGCGCTCCCC
  CTTTAAGGGGCCCCCTCCCCCCCCGGAAACCCACCCTCTTGGGGAGAAATTTCCCCACAA
  AAGAGACCCCCCCCGCCTTTGGGGCCCCCCGGGGGGGGGGGGGCCCCCCCCCCATATACC
  CCCCCCCCACCGGAAAAAAAACAAAAAAAATTTTGGCCCACCCCCCCAAAAAAAACCCCC
  CCCCCGGGGGCACACCCTTTAAAATTTTTCCCCCCCGGGAGGGGGGCCCCCCCGGGGGGA
  GCCCCCCCCAAAAAAAAATTTTTTTCTTCCCCCCCCCCCCAAGAAAAAAAAAATTTCCTC
  TCCCCCACAAGAGGGGCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002634
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002634.2, NP_002625.1
• RefSeq Size: 1826 bp
• RefSeq ORF: 819 bp
• Locus ID: 5245
• UniProt ID: P35232
• Cytogenetics: 17q21.33
• Domains: Band_7
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 4 encode the same isoform (1).