POLR2J2 (NM_032959) Human Untagged Clone

SKU SC110811

POLR2J2 (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide J2 (POLR2J2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: POLR2J2
• Synonyms: HRPB11B; POLR2J3; RPB11b1; RPB11b2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110811 sequence for NM_032959 edited (data generated by NextGen Sequencing); ATGAACGCCCCTCCAGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCGAGAAGATCACCATT; AACAAGGACACCAAGGTACCCAATGCCTGTTTATTCACCATGAACAAAGAAGACCACACA; CTGGGAAACATCATTAAATCACAACTCCTAAAAGACCCGCAAGTGCTATTTGCTGGCTAC; AAAGTCCCCCACCCCTTGGAGCACAAGATCATCATCCGAGTGCAGACCACGCCGGACTAC; AGCCCCCAGGAAGCCTTTACCAACGCCATCACCGACCTCATCAGCGAGCTGTCCCTGCTG; GAGGAGCGCTTCCGGACGTGCCTGCTTCCCCTTCGCCTTCTGCCGTGA; ; Clone variation with respect to NM_032959.5; 84 g=>t;90 c=>t;102 c=>g;285 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032959 unedited; TTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAGGGGACACTTGGGGTCTGGA; CGCAACGGCGGCGGGAGCATGAACGCCCCTCCAGCCTTTCGAGTCGTTCTTGCTCTTCGA; GGGCGAGAAGATCACCATTAACAAGGACACCAAGGTACCCAATGCCTGTTTATTCACCAT; GAACAAAGAAGACCACACACTGGGAAACATCATTAAATCACAACTCCTAAAAGACCCGCA; AGTGCTATTTGCTGGCTACAAAGTCCCCCACCCCTTGGAGCACAAGATCATCATCCGAGT; GCAGACCACGCCGGACTACAGCCCCCAGGAAGCCTTTACCAACGCCATCACCGACCTCAT; CAGCGAGCTGTCCCTGCTGGAGGAGCGCTTCCGGACGTGCCTGCTTCCCCTTCGCCTTCT; GCCGTGATTGTCAGTTTCCTGAGGCCTCCCCAGCCACGCTTCCTGTACAGCCTGCAGAAC; TTCTGGCATGGAAAAGCCCCTGTGCAACTGGTAAAGATATCAATAAGCACCAGGAGGTAT; CTAAATCCACCAGGAGCCATAGGCATCACGNTTGACGTCCATTTACCAGTCTTCCCTGGC; AAGATTCTTCTGAATTGTGCTGCCTTGCCAAAGAAGTTTGGAAGGGGCCTGGGCCCAATG; GGCTTGGGCCTTGAATCCCAACATTTTGGGAAACAATTCAAGTGGGATGTTATAAGGGCA; AAGGGGTTCAGAACATCCTGTCCAACATGGTGACATTCCATCTCTACTAAAATACATAAG; TAACTGGNNTTGNNNGNNNGGGGGGCCCCGGTGATCCCCCCTCCTCCAAAAGGTGAGGGC; AGATAATCGCTTGACCTGGAGGGAAGAGGGGGGGGGGGGACCNAAAAATATGGCCTTTTG; CTCCCACGGGGGGAAAAAAAGGAAAAACTCCCTTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_032959
• Insert Size: 2060 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032959.3, NP_116581.3
• RefSeq Size: 1727 bp
• RefSeq ORF: 348 bp
• Locus ID: 246721
• UniProt ID: Q9GZM3
• Cytogenetics: 7q22.1
• Domains: RNA_pol_L
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Summary: This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]