SPSB1 (NM_025106) Human Untagged Clone

SKU SC110780

SPSB1 (untagged)-Human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SPSB1
• Synonyms: SSB-1; SSB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110780 sequence for NM_025106 edited (data generated by NextGen Sequencing); ATGGGTCAGAAGGTCACTGGAGGGATCAAGACTGTGGACATGAGGGACCCCACGTACAGG; CCCCTGAAGCAGGAGCTCCAGGGTCTGGATTACTGCAAGCCCACCCGGCTGGATCTGCTA; CTGGACATGCCCCCTGTGTCCTATGATGTCCAGCTGCTGCATTCATGGAACAACAACGAC; CGATCGCTCAATGTCTTTGTAAAGGAGGACGACAAGCTCATCTTTCACCGGCATCCGGTG; GCCCAGAGCACGGACGCTATCAGGGGCAAAGTCGGGTATACCCGTGGGCTGCACGTGTGG; CAGATCACGTGGGCCATGAGACAGCGGGGCACACACGCCGTGGTGGGGGTGGCGACGGCA; GACGCCCCCCTGCACTCTGTCGGGTACACAACCCTCGTGGGGAATAACCACGAGTCCTGG; GGCTGGGACTTGGGGCGCAACCGGCTCTACCACGATGGCAAGAACCAGCCAAGCAAAACA; TACCCAGCCTTTCTGGAACCAGATGAGACATTCATTGTCCCTGACTCCTTCCTGGTAGCC; CTGGACATGGACGACGGGACTCTGAGCTTCATTGTGGATGGACAGTACATGGGAGTGGCT; TTTCGGGGACTCAAGGGCAAAAAACTGTATCCTGTAGTGAGTGCCGTCTGGGGCCACTGT; GAGATCCGAATGCGCTACTTGAACGGACTCGATCCCGAGCCGCTGCCGCTCATGGATTTG; TGCCGTCGCTCGGTGCGCCTGGCCCTGGGGAGGGAGCGCCTGGGGGAGATCCACACGCTG; CCGCTGCCGGCTTCCCTCAAGGCCTACCTCCTCTACCAGTGA; ; Clone variation with respect to NM_025106.3; 201 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025106 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTCCTGGCAGCCCTCA; TTAGGAATTCTGTCTGGCCCCGATCAGAATACTGGAGACGAGACCACGAGATTGATGAGT; TTGCCTTGGGAGTCGGTAAGAAGGTGAAGCCAGGGGCGAACATGGGTCAGAAGGTCACTG; GAGGGATCAAGACTGTGGACATGAGGGACCCCACGTACAGGCCCCTGAAGCAGGAGCTCC; AGGGTCTGGATTACTGCAAGCCCACCCGGCTGGATCTGCTACTGGACATGCCCCCTGTGT; CCTATGATGTCCAGCTGCTGCATTCATGGAACAACAACGACCGATCGCTCAATGTCTTTG; TAAAGGAGGACGACAAGCTCATCTTTCACCGGCATCCGGTGGCCCAGAGCACGGACGCTA; TCAGGGGCAAAGTCGGGTATACCCGTGGGCTGCACGTGTGGCAGATCACGTGGGCCATGA; GACAGCGGGGCACACACGCCGTGGTGGGGGTGGCGACGGCAGACGCCCCCCTGCACTCTG; TCGGGTACACAACCCTCGTGGGGAATAACCACGAGTCCTGNGGCTGGGACTTGGGGCGCA; ACCGGCTCTACCACGATGGCAAGAACCAGCCAAGCAAAACATACCCAGCCTTTCTGGAAC; CAGATGAGACATTCATTGTCCCTGACTCCTTCCTGGTAGCCCTGGACATGGACGACGGGA; CTCTGAGCTTCATTGTGGATGGACAGTACATGGGAGTGGCTTTTCGGNGACTCAAGGGCA; NAAACTGTATCCTGTANTGAGTGCCGTCTGGGCCACTGTGAGATCCGATGCGCTACTTGA; ACGACTCGATCCCGACCGCTGCCGCTCATGGATTGTGCCCGTCGCTCGTGCGCTGGNCCT; GGGNAGGGGACGCCTGGGGGAGATCACCGCTGCCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025106 unedited; NCCCTTCTCTGNNACCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGATA; TTTTAACAAACGTTCCTTTATTAGGTTACTGACTATTATCAACCATCATAAAATAGAACG; GGAGTTTCAAAACTGTACAAGCCACAAGGCTCTGGGCTGGTAGGAAAGAAGGGTGGGGTC; GAGGGTCCCAGGGTGTCGGGGGGTGGGAGATGCAGAGAGAGCTAGAGGGTCACCCGGCAT; CTGTGAGGACGGCTGGGTCAAGGCCATAAGCTGGGATCTGTACAAGGGAAACATTCATCA; GAATGTGACCCACCTGAAACAGGAGGGAGGAAAATCTTTAAAAGTCTTACAGGTAAGGTC; CCCTGCCCCGAAAAAAAAAACCGTCAAAATAATAAGGGGGTAATGTACATTTCTCACCCA; GTCTTGGCACCAATTTTGTGCTTTAAAAAATATACTCCACTGTAAGATTTACTTAAAAAA; AGGTACTCTACAGCAGCTGTTTAAAACATAATTCTTACAGACAAATATATATATATGTAT; GTATATATATTAAACATTTTAAACAAATAAAGTCATCTATTGTACCTGTGCAGAAATGAA; CCAAAGTTAGACTCTAAGAGAGTTAACTTCAAGCTCAAAGAAATGGCAAAGGCGGTGGCA; GGTGCTGGGGTACGCTACGTGGAGGGGTTGCCAGGGATAGGGCACCCCTCTCTTTNCGCA; GCCGAGCTACCCCTCTTTGAGCTCTGANAGGGGGTNGGGATGAACTGNNGCTGTTACCCC; TGGTCCANAGCCCCNGCTGGGGTGCTGAGCANAGACCGCGCTGGCTTCTGTGAGGCTCGA; CTCTGCTCTTGGTCTTCCCATCCCACCTGCCCNTGCTCTCGGGGAATGAGACCCCTCGCC; TCCTTCTAGTCTGGGGGCCTCTGCCCACCAGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_025106
• Insert Size: 2890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025106.2, NP_079382.2
• RefSeq Size: 3081 bp
• RefSeq ORF: 822 bp
• Locus ID: 80176
• UniProt ID: Q96BD6
• Cytogenetics: 1p36.22
• Domains: SPRY
• Protein Families: Druggable Genome
• Summary: Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820, PubMed:21199876). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876).[UniProtKB/Swiss-Prot Function]