DIABLO (NM_138929) Human Untagged Clone

SKU SC110581

DIABLO (untagged)-Human diablo, IAP-binding mitochondrial protein (DIABLO), nuclear gene encoding mitochondrial protein, transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: DIABLO
• Synonyms: DFNA64; DIABLO-S; SMAC; SMAC3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138929, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCTCTGAAGAGTTGGCTGTCGCGCAGCGTAACTTCATTCTTCAGGTACAGACAGTGTTTGTGTG; TTCCTGTTGTGGCTAACTTTAAGAAGCGGTGTTTCTCAGAATTGATAAGACCATGGCACAAAACTGTGAC; GATTGGCTTTGGAGTAACCCTGTGTGCGGTTCCTATTGCACAGGCTGTTTATACCTTAACTTCTCTTTAC; CGACAATATACAAGTTTACTTGGGAAAATGAATTCAGAGGAGGAAGATGAAGTGTGGCAGGTGATCATAG; GAGCCAGAGCTGAGATGACTTCAAAACACCAAGAGTACTTGAAGCTGGAAACCACTTGGATGACTGCAGT; TGGTCTTTCAGAGATGGCAGCAGAAGCTGCATATCAAACTGGCGCAGATCAGGCCTCTATAACCGCCAGG; AATCACATTCAGCTGGTGAAACTGCAGGTGGAAGAGGTGCACCAGCTCTCCCGGAAAGCAGAAACCAAGC; TGGCAGAAGCACAGATAGAAGAGCTCCGTCAGAAAACACAGGAGGAAGGGGAGGAGCGGGCTGAGTCGGA; GCAGGAGGCCTACCTGCGTGAGGATTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138929 unedited; TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGGTCTGGCG; TCCGCGCGCTGCACAATGGCGGCTCTGAAGATTGGCTGTCGCGCAGCGTAACTTCATTCT; TCAGGTACAGACAGTGTTTGTGTGTTCCTGTTGTGGCTAACTTTAAGAAGCGGTGTTTCT; CAGAATTGATAAGACCATGGCACAAAACTGTGACGATTGGCTTTGGAGTAACCCTGTGTG; CGGTTCCTATTGCACAGAAATCAGAGCCTCATTCCCTTAGTAGTGAAGCATTGATGAGGA; GAGCAGTGTCTTTGGTAACAGATAGCACCTCTACCTTTCTCTCTCAGACCACATATGCGT; TGATTGAAGCTATTACTGAATATACTAAGGCTGTTTATACCTTAACTTCTCTTTACCGAC; AATATACAAGTTTACTTGGGAAAATGAATTCAGAGGAGGAAGATGAAGTGTGGCAGGTGA; TCATAGGAGCCAGAGCTGAGATGACTTCAAAACACCAAGAGTACTTGAAGCTGGAAACCA; CTTGGATGACTGCAGTTGGTCTTTCAGAGATGGCAGCAGAAGCTGCATATCAAACTGGCG; CAGATCAGGCCTCTATAACCGCCAGGAATCACATTCAGCTGGTGAAACTGCAGGTGGAAG; AGGTGCACCAGCTCTCCCGGAAAGCAGAAACCAAGCTGGCAGAAGCACAGATAGAAGAGC; TCCGTCAGAAACACAGNANGAAAGGGGAGGAGCGGGCTGAGTCNGAGCAGGAGGCCTACC; TGCGTGAGGATTGAGGGCCTGAGCCACTGCCCTGTCTCCCACTCAGTGGGAAAGCAGGNG; CAGATGCCACCCTGCCANGGGTTGCATGACTGTCTGTGCACCGAGAGAGGCCGCAGATCC; TGNCCTGGNCATCAGCGAGAGCCTTTGNAGCTTGGAGTGCTNCTGTGGTCTAGGCTGCCT; GGACCTGGTCTTANCCTTGGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138929 unedited; TCTGAACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTAAAACCCTGTTGATGTTAAACTCCTGTTGAAAAG; CACCAGGTAAACACTCTGCACCCCTTTTTTTAAAAAAAAAAGGTTTTTCACTCCTTGGCC; TCAGCTGTCCTCACAGGACAGGGGGGGCAGATCAAAAAACACATTCAAAAATACCTACAA; AAAAATCGTACAAACTGGACAGGTTCCCCTCCCCCTGCCACAACTGGCATCCCAACAGAG; GGAACAAGTCCTAAATCTTTTTTGACGACGTAAATAAAACTGAAAACAGGTTAAACAGTT; GCTGAACTTAAGGGCATGACAAAAAGGACTCCTTTCTTTGACCCAGGTAGGCAAAATGCT; TTGGGGGGGAGGAAAAAAAAATGGGTAAAACCAGCTGTCCAAAGGGGGGGGAAATGTTAA; ACAGGGTGCAGGGCCCAAGGGCTAAAAACCAGGTCCAGCGCAAGCCTGAAACCACAGGAG; GCACTCACAGCTCACAAAGGCGTTTTGCCTGATTGGCCAGGGCAGGATCTGCCGCCTCTT; TTTGGTGCACAAACAGTCATGCCAACCCTGGGCAGGGTGGAATTTGCCCCTGTTTTCCCC; ACTGAGTGGGGAAACAGGGCAGTGTGCCTCAGCCCTAATTCCTAACGCAGGTAGGCCCTC; CTGTTCCAAATAAAACCCGTTTCTCCCCTTCCTCCTGTGTTTTTTGAGGGAGCTCTTTTA; TTTGGGCTCCGGCCACACTGGTTTTTCGTTTTTCGGGAAAAGTGGGGCACCCTTTTCCCT; CGGAGTTCCACCACCGAGAAGGAATCCCGGCCGGTTATAAACGCCCTGACTGCCCCCATT; TAAATCCACCTTTGTTGCCCTTTCTAAAAACCACCTGGACTCCCCAGGTGTTCCCACTTC; AAACCTTTTGAGGTTTGAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_138929
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138929.2, NP_620307.1
• RefSeq Size: 1366 bp
• RefSeq ORF: 588 bp
• Locus ID: 56616
• Cytogenetics: 12q24.31
• Protein Families: Transmembrane
• Summary: This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]; Transcript Variant: This variant (3) lacks an in-frame segment, compared to variant 1. The resulting isoform (3) is shorter than isoform 1 and retains only a small portion of the IAP-binding domain. The isoform was previously identified as Smac-delta.